In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application

Kumar, Brajesh; Smita, Kumari; Seqqat, Rachid; Benalcazar, Karen; Grijalva, Marcelo; Cumbal, Luis

doi:10.1016/j.jphotobiol.2016.03.011

Cited by 103 publications

(51 citation statements)

References 30 publications

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“…Hydrogen peroxide assay of the EAENH and its biosynthesized AgNPs was performed (Kumar et al, 2016). In this assay, different concentrations (20 -100 μg/mL) of the samples were used, and ascorbic acid was utilized as standard.…”

Section: Hydrogen Peroxide Radical Scavenging Assaymentioning

confidence: 99%

Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities

Gnanakani

Santhanam

Premkumar

et al. 2019

Asian Pac J Cancer Prev

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Objective: To investigate the biogenic synthesis of silver nanoparticles (AgNPs) using partially purified ethyl acetate extract of Nannochloropsis sp. hexane (EAENH) fraction of microalga. Methods: The green synthesis of AgNPs was confirmed with UV-Vis spectrum which shows the surface plasmon resonance (SPR) at 421 nm. Fourier Transform Infrared Spectra (FTIR) presented the involvement of functional groups like carboxyl groups of fatty acids, tetraterpenoids of xanthophylls, hydroxyl groups of polyphenols, carbonyl and amide linkage of proteins in the AgNP synthesis. Gas Chromatography-Mass Spectrometry analysis (GCMS) revealed that phytochemicals like octadecanoic acid and hexadecanoic acid imply in capping, bioreduction, and stabilization of AgNps. Result: High-resolution Transmission electron microscope (HRTEM), Dynamic light scattering (DLS), X-ray diffraction (XRD) and EDX analysis showed the crystalline form of the AgNPs with Z-average size 57.25 nm. The zeta potential value of-25.7 mV demonstrated the negative surface charge and colloidal stability of AgNPs. The antimicrobial activity of AgNPs displayed effective inhibition zone against selected bacterial and fungal pathogens. In vitro, antioxidant effects were assessed by 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydrogen peroxide and reducing power assays which revealed excellent scavenging potential for AgNPs than the extracts. The anti-proliferative potential of biofabricated AgNPs and extracts on Human Non-small lung cancer cell line (A549) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyl-tetrazolium bromide (MTT) assay with IC 50 values of 15 µgmL-1 and 175 µgmL-1 respectively. Conclusion: The study reveals that the microalgae-mediated AgNPs possesses potent antimicrobial and antioxidant activity along with the ability to stimulate apoptosis in A-549 cell line.

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Section: Hydrogen Peroxide Radical Scavenging Assaymentioning

confidence: 99%

Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities

Gnanakani

Santhanam

Premkumar

et al. 2019

Asian Pac J Cancer Prev

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“…Four well-defined characteristic diffraction peaks at 39.0°, 44.0°, 65.0°, and 78.0°, corresponding to the (111), (200), (220), and (311) planes of the fcc crystal structure of metallic silver (Fig. 7) were observed for white, green, red, blue LED light and sunlight, respectively [36,37]. The standard XRD patterns of Ag are similar (Joint Committee on Powder Diffraction Standards file no: a = 4.086 Å; JCPDS PDF 04-0783).…”

Section: X-ray Diffractionmentioning

confidence: 99%

Photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity

Lee¹,

Lim

Velmurugan

et al. 2016

Journal of Photochemistry and Photobiology B: Biology

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“…Among the noble metals, silver and gold have been a focus of interest for pharmacological bio-efficacies (Elia et al 2014; Fatimah 2016). Silver, in particular, has potent antimicrobial activity including antifungal, anti-oxidant, anti-inflammatory, and wound healing (Kumar et al 2016). Further, bimetallization can often surpass the enhancement of the catalytic properties of the original single metal, which may not be achieved by monometallic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Biofabrication and characterization of flavonoid-loaded Ag, Au, Au–Ag bimetallic nanoparticles using seed extract of the plant Madhuca longifolia for the enhancement in wound healing bio-efficacy

et al. 2019

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The present communication warrants the presence of significant wound healing bio-efficacy of aq. alc. extract of the seed (49.78%) of the plant Madhuca longifolia. A family of seven flavonoid fractions have been ascertained in the seed aq. alc. extract of the target plant using LCMS-8030 analysis. In vivo wound healing parameters (wound area, wound closure, epithelization period, skin breaking strength and hydroxyproline content) have been examined in Swiss albino mice models. Statistically significant (p < 0.001) enhancement in the wound healing bio-efficacy has been effectively induced using flavonoid-loaded gold: (Mlf@AuNps), silver: (Mlf@AgNps), and Au–Ag bimetallic: (Mlf@Au–AgNps) nanoparticles. Among the biofabricated nano-biomaterials, Mlf@AgNps exhibited an exceptional enhancement in the wound healing bio-efficacy (80.33%) attaining almost to the level of reference drug Placentrex (84.02%). All the fabricated nano-biomaterials were thoroughly characterized using UV–Vis, XRD, FE-SEM, TEM, EDX, and DLS. The promising enhancement in the wound healing potential of the nano-biomaterial (Mlf@AgNps) has been explained based on the cumulative effects of biological and nanotech parameters. The bio-fabricated (Mlf@AgNps) nano-biomaterials using the plant M. longifolia have lustrous prospects for the development of complimentary herbal nanomedicine for scaling-up the wound healing bio-efficacy.Graphical abstract

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In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application

Cited by 103 publications

References 30 publications

Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities

Nannochloropsis Extract–Mediated Synthesis of Biogenic Silver Nanoparticles, Characterization and In Vitro Assessment of Antimicrobial, Antioxidant and Cytotoxic Activities

Photobiologic-mediated fabrication of silver nanoparticles with antibacterial activity

Biofabrication and characterization of flavonoid-loaded Ag, Au, Au–Ag bimetallic nanoparticles using seed extract of the plant Madhuca longifolia for the enhancement in wound healing bio-efficacy

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