In vitro evaluation of the antimicrobial activity of endodontic sealers

Miyagak, Daniela Cristina; Carvalho, Elaine Manso Oliveira Franco de; Robazza, Carlos Roberto Colombo; Chavasco, Jorge Kleber; Levorato, Gustavo Labegalline

doi:10.1590/s1806-83242006000400004

Cited by 65 publications

(63 citation statements)

References 19 publications

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“…However, in this study all materials were ineffective against E. faecalis, as similar to those observed in the literature related to Estrela et al 4 , Ribeiro et al 13 , Zarrabi et al 14 and Miyagak et al 24 , in constrast to the results presented in Sipert et al 11 and Tanumaru Filho et al 12 investigations.…”

Section: Discussionsupporting

confidence: 92%

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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ObjectiveThe objective of this study was to evaluate the antimicrobial activity of Mineral trioxide aggregate e Portland cement against some selected cariogenic bacteria. Methods Wells were made of approximately 0.5 mm in diameter, in solid culture media and immediately filled with cement. Twelve samples of each material were obtained for the realization of the agar diffusion method. These samples were tested with Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus (UFV) and Lactobacillus casei (UNICAMP). Petri plates containing Tripticase soy Agar (TSA) were used for the growth of Streptococcus mutans and Enterococcus faecalis, and plates of Man Rogosa & Sharpe Agar (MRS) for the Lactobacillus acidophilus and Lactobacillus casei. After 48 hours of incubation, it was made the measurement of inhibition halos with the aid of manual caliper. ResultsThe diameters obtained to MTA (2.50 ± 0.00;2,92 ± 0.63; 2.58 ± 0.38), PC (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) and calcium hydroxide cement (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) ResultadosOs diâmetros obtidos do MTA (2.50 ± 0.00; 2,92 ± 0.63; 2.58 ± 0.38), CP (1.17 ± 0.29; 2.00 ± 0.43; 1.33 ± 0.14) e cimento de hidróxido de cálcio (3.83 ± 0.29; 3.00 ± 0.00 ± 2.58 ± 0.72) foram expressos em milímetros e submetidos ao teste de Tukey (p<0.05). Conclusão METHODSThe tested materials were: MTA (Angelus Soluções Odontológicas, Londrina, Brasil), Portland cement (CP II -F32, ITAPESSOCA AGRO-INDUSTRIAL S.A., Goiana, Brasil) and calcium hydroxide paste (Reagen TM).The antimicrobial activity of the materials was evaluated by the Agar diffusion method against four different strains.The strains used for analysis were Streptococcus mutans (ATCC 700610), Enterococcus faecalis (ATCC 29212), Lactobacillus acidophilus and Lactobacillus casei isolated by the Laboratory of Microbiologic Science (Federal University of Viçosa -MG (UFV) and University State of Campinas -UNICAMP, SP, Brazil), respectively.Of each bacterial culture tested, it was held a concentration suspension of approximately 5x108 of colonies per mL, corresponding to 0.5 of Mc Farland em range of sterile medium Trypticase Soy Browth (TSBMerck). The Streptococcus mutans and Enterococcus faecalis suspensions were inoculated with spread plate in the middle of Sodium Tioglicolate (Merck) culture and Lactobacillus and Lactobacillus acidophilus casei were inoculated in the culture medium Man Rogosa & Sharpe Agar (MRS-HIMEDIA). Subsequently, the plates were incubated at 35 ± 1°C for 24/48h and the halos of inhibition were expressed in millimeters, using a caliper manual.A total of 12 plates were employed, each microorganism was tested in triplicate. Three cavities, measuring 4mm in diameter were made in each agar plate using a copper puncher and then completely filled with freshly manipulated product to be tested.Positive (CaOH) and negative controls (sterile growing medium) were tested using the same methodology and under the same conditions of the experiment.All cult...

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Section: Discussionsupporting

confidence: 92%

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Júnior

Sobral

Sampaio

et al. 2015

RGO, Rev. Gaúch. Odontol.

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“…Several studies have shown that MTA does not have any significant growth inhibiting effect on a number of oral bacteria. 36,37 In contrast to these findings, Eldeniz et al,38 evaluating the antibacterial activity of leachable components from selected rootend filling materials, found that set samples of ProRoot MTA cement completely inhibited Pseudomonas aeruginosa and delayed or limited growth of Enter. faecalis.…”

Section: Discussionmentioning

confidence: 98%

Sealing ability of mineral trioxide aggregate, glass ionomer cement and composite resin when repairing large furcal perforations

Lodienė¹,

Kleivmyr

Bruzell

et al. 2011

Br Dent J

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“…The dissociation into calcium and hydroxide ions results in an increase in the pH and calcium ion release 22) . The antimicrobial effects of MTA have been widely assessed, but contradictory findings have been reported 3,18,[23][24][25][26] . An in vitro study showed that MTA exerts antibacterial effects against some facultative bacteria and but not on any species of absolute anaerobes 3) .…”

Section: Discussionmentioning

confidence: 99%

“…Some studies have shown that white MTA 25) and grey MTA 23,24) exert antifungal effects. However, others have shown that grey MTA has limited or no antifungal effects 19,26) . Torabinejad et al 27) reported that the pH of MTA is 10.5 at the time of mixing but 12.9 after 3 h. According to McHugh et al 28) , E. faecalis is unable to survive at pH values of 11.5 or greater.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial effects of several calcium silicate-based root-end filling materials

et al. 2014

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The purpose of this study was to evaluate, in vitro, the antimicrobial effect of iRoot BP, iRoot BP Plus, and mineral trioxide aggregate (MTA) against Entercoccus faecalis and Candida albicans by using direct contact test. The materials were tested immediately after application to the microtiter wells and after setting for 1-day and for 7-days. Ten microliters of microbial suspension was added to each well for direct contact with each material for 1 h at 37°C and 100% humidity. Then fresh media was added and, survival of bacteria and fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. In fresh and 1-day samples all of tested materials showed statistically significant antimicrobial effects compared to control groups (p<0.05). In 7-day samples, there were no significantly differences compared to control groups. MTA, iRoot BP and iRoot BP Plus had similar antimicrobial efficacy against E. faecalis and C. albicans.

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In vitro evaluation of the antimicrobial activity of endodontic sealers

Cited by 65 publications

References 19 publications

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Evaluation of cariogenic antibacterial activity of mineral trioxide aggregate and Portland cement

Sealing ability of mineral trioxide aggregate, glass ionomer cement and composite resin when repairing large furcal perforations

Antimicrobial effects of several calcium silicate-based root-end filling materials

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