In vitro evaluation of the probiotic potential of Lactobacillus isolated from native swine manure

Abstract

Background and Aim: Using antimicrobials as a feed additive in swine production is prohibited because it is a major cause of the emergence of antimicrobial-resistant bacteria. Probiotics such as Lactobacillus spp. are an attractive alternative to reduce antimicrobial resistance and promote swine growth. This study aimed to evaluate the in vitro probiotic properties of Lactobacillus isolated from indigenous swine manure. Materials and Methods: A total of 30 fecal samples from healthy individual indigenous pigs were collected and isolated on de Man, Rogosa, and Sharpe agar. The preliminary screen identified candidates with antibacterial activity against six pathogens and >50% survival and tolerance to acid (pH 3.0) and 1% bile salt. Isolates that passed the initial screen will be tested for other probiotic properties. Results: Of the 314 isolates from 30 pig manure samples, 17 isolates satisfied all initial conditions for probiotic properties. Each isolate has unique, distinctive properties. Isolates B4, B5, B8, B17, B87, and B144 formed thick biofilms, whereas isolates B5, B8, and 27 adhered well to the intestinal wall and exhibited strong autoaggregation properties. Isolate B4 aggregated with Enterohemorrhagic Escherichia coli and Enteropathogenic E. coli. Tests in pH-adjusted cell-free medium indicated that the antibacterial activity resulted from bacterial acidification rather than bacteriocin formation. Sequence analysis (16S rRNA) revealed 16 of the isolates were Lactobacillus plantarum, and only one isolate was Lactobacillus salivarius. Conclusion: We isolated 17 Lactobacillus from swine manure and demonstrated that their probiotic properties might be useful as a probiotic cocktail for swine feed.

Genomic characterization and probiotic potential of lactic acid bacteria isolated from feces of guinea pig (Cavia porcellus)

“…Second, the observed halos in this study signify a manifestation of alpha hemolysis on blood agar ( Halder et al , 2017 ). Consistent with these findings, prior investigations have reported analogous hemolytic activity in lactic acid bacteria isolated from the gastrointestinal tracts of poultry and swine ( Tuyarum et al , 2021 ; Makzum et al , 2023 ). The degradation of blood agar is posited to be instigated by hemolysin, an enzymatic agent capable of lysing host cells to facilitate the assimilation of ferrous compounds, particularly hemoglobin ( Chen et al , 2018 ).…”

Whole-Genome Sequencing, Phylogenetic and Genomic Analysis of Lactiplantibacillus pentosus L33, a Potential Probiotic Strain Isolated From Fermented Sausages

“…Moreover, in several LAB species, the D-alanine residue located at the end of the pentapeptide is substituted by D-lactate or D-serine and thus blocking vancomycin binding ( Delcour et al, 1999 ). KEGG analysis showed that L. pentosus L33 encodes a Ddl ligase, responsible for the D-alanine to D-lactate substitution in Lactobacilli ( Tuyarum et al, 2021 ). Furthermore, there are reports that indicate that many LAB genera exhibit intrinsic resistance to other antibiotics, such as bacitracin, kanamycin, teicoplanin, and quinolones ( Imperial and Ibana, 2016 ).…”

Multifunctional Probiotic and Safety Attributes Heyndrickxia coagulans Isolated from Stingless Bee Honey

“…The two H. coagulans strains exhibited a remarkable ability to withstand acidic conditions, with survival rates surging to 300% at pH 3.0 over 3 hours, compared to the control strain, L. plantarum (B-17) isolated from a previous study (Tuyarum et al, 2021). This indicates their aptitude to thrive in low-pH environments.…”

“…The displayed acid and bile salt tolerance has potential industrial applications, given the need for probiotics to survive the stomach's highly acidic environment and colonize within the intestine. The capacity to withstand acidic and saline environments is vital for speci c probiotic bacteria, allowing them to endure extreme conditions and establish themselves within the gastrointestinal tract (Tuyarum et al, 2021). These processes facilitate bacterial respiration by transporting protons, indicating that F0F1-ATPase may elevate the cell's pH in acidic conditions through gene expression regulation.…”

“…The Lactobacillus plantarum (B17) from previous studies that were found to be able to withstand acids and bile salts at 100% is positive control (Tuyarum et al, 2021). The bacterial survival rates at these two time points were determined through the single plate-serial dilution spotting technique (SP-SDS) (Thomas et al, 2015).…”