In vitro evaluation of the effect of the buckwheat protein hydrolysate on bacterial adhesion, physiology and cytokine secretion of Caco-2 cells

Świątecka, D; Markiewicz, Lidia Hanna; Wróblewska, Barbara

doi:10.5114/ceji.2013.37753

Cited by 13 publications

(5 citation statements)

References 37 publications

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“…The construction of the standard curve for each real-time PCR analysis was based on bacterial strains from our own culture collection and from the German Collection of Microorganisms and Cell Cultures. Each bacterial culture was separately cultivated in appropriate conditions (details available upon request), and the cell count was determined using 4′,6-diamidino-phenylindole (Sigma-Aldrich) with a procedure described elsewhere [21]. Two millilitres of each culture was centrifuged (5 min, 10,000× g ), washed with sterile PBS (pH 7.4) and then centrifuged again.…”

Section: Methodsmentioning

confidence: 99%

Metabolism of strawberry mono- and dimeric ellagitannins in rats fed a diet containing fructo-oligosaccharides

et al. 2015

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PurposeWe investigated the effects of dietary supplementation with strawberry extracts rich in ETs and fructo-oligosaccharides (FOS) on the intestinal microbiota and the formation of bacterial metabolites in the distal intestine, as well as the absorption of ET metabolites and antioxidant status in rats.MethodsRats were allocated into six groups of eight animals each and fed for 4 weeks with a control diet (group C), a control diet supplemented with FOS (group C + FOS) or modifications of these diets, in which a monomeric or dimeric ET-rich extract was added (groups ME and ME + FOS or DE and DE + FOS, respectively).ResultsThe extract addition, the FOS addition and their interaction significantly affected the total and selected bacterial counts in the caecal digesta (all P < 0.005). The total bacterial count was the highest in group C + FOS, lower in group DE and the lowest in group ME + FOS (10.6, 10.3 and 8.52 log cells/g, respectively; P ≤ 0.05). The total caecal content of ET metabolites was higher in the ME and ME + FOS group than in the DE and DE + FOS group, respectively (67.8 and 89.5 vs. 13.0 and 18.0 µg/g, respectively; P < 0.001). The total plasma concentration of ET metabolites was higher in the ME + FOS and DE + FOS group than in the ME group (248 and 281 vs. 8.13 ng/mL, respectively; P < 0.001).ConclusionsETs of the monomeric ET-rich extract are more prone to intestinal breakdown than those of the dimeric ET-rich extract, and absorption of their metabolites can be increased by dietary FOS; however, together, they evoke strong antibacterial activity.

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Section: Methodsmentioning

confidence: 99%

Metabolism of strawberry mono- and dimeric ellagitannins in rats fed a diet containing fructo-oligosaccharides

et al. 2015

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“…While rutin was the most abundant flavonoid measured, it did not show significant cytotoxicity. The studies of Ishii et al [ 29 ], and Swiatecka et al [ 36 ], used colon cancer lines to test buckwheat-derived products but showed conflicting results. Ishii et al [ 29 ] compared the LPS-induced response from human carcinoma cell lines (CoLoTC) and extracts from buckwheat.…”

Section: Resultsmentioning

confidence: 99%

“…They used IL8 as a biomarker for inflammation and found that the buckwheat extracts significantly lowered IL8 expression in colon cancer cells in a dose-dependent manner but did not influence cell viability. On the other hand, Swiatecka et al [ 36 ] tested buckwheat protein hydrolysate on the CaCo-2 cell line, resulting in significantly lower cell proliferation and significantly higher levels of IL8. In another study, Kim et al [ 37 ] prepared extracts from buckwheat hull with multiple fractions of different solvents (ethanol, ethyl acetate, hexane, butanol, chloroform, and water) and tested them to multiple cancer lines (human gastric carcinoma, human hepatocellular, carcinoma, human lung carcinoma, human breast adenocarcinoma, and human cervical adenocarcinoma).…”

Section: Resultsmentioning

confidence: 99%

Systematic Review of Human and Animal Evidence on the Role of Buckwheat Consumption on Gastrointestinal Health

et al. 2022

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Background: Buckwheat is a commonly cultivated crop with growing evidence that it is beneficial to gastrointestinal (GI) health. This systematic review summarizes the role of buckwheat in modifying GI health outcomes and microbiomes. Methods: Four medical databases and Google Scholar were systematically searched. Clinical trials, observational studies, animal in vivo, and in vitro studies with human and animal GI-derived samples were included. Results: There were 32 studies (one randomized controlled trial [RCT], one non-randomized trial, 3 observational, 9 in vitro, and 18 animal in vivo studies) included. In preclinical studies, buckwheat extracts were observed to have cytotoxic potential against human-derived GI cancer cell lines. Animals fed with buckwheat had lower GI mucosal inflammation, higher alpha diversity in the GI microbiome, and higher levels of fecal short-chain fatty acids. Human evidence studies and clinical trials were limited and predominantly of moderate risk of bias. The majority of in vitro studies with GI-derived samples and in vivo studies were reliable without restrictions in study design. Conclusion: In vivo and in vitro studies show that buckwheat may have potential GI benefits due to its anti-oxidant and anti-inflammatory potential; however, human evidence remains limited, and its impact on health in humans remains to be elucidated in future trials.

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“…The IL-8 production by intestinal cells in response to bacterial stimuli depends on the specific epitopes present on bacterial surface, such as pathogen-associated molecular patterns (PAMPs) being ligands for TLRs. Activation of different TLRs by complex microbial consortia activates the innate immune response, and the final direction of immune response (pro- or anti-inflammatory) depends on the specific composition of the microbial consortium [ 36 , 37 , 38 , 39 ]. In our study, there was no significant difference in IL-8 secretion when compared healthy and cancer colonocytes treated only with V or O bacteria ( Figure 3 ).…”

Section: Discussionmentioning

confidence: 99%

Phytate Hydrolysate Differently Modulates the Immune Response of Human Healthy and Cancer Colonocytes to Intestinal Bacteria

et al. 2022

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(1) Phytic acid (PA) is a component of cereal seeds and legumes, therefore its consumption is much higher in a vegan and vegetarian diet compared to a conventional diet. The diet is the main driver of metabolic activity of gut microbiota, therefore, the ability to degrade phytates by the microbiota of vegans significantly exceeds that of the gut microbiota of omnivores. The aim of the study was to investigate the early phase of the immune response of colonocytes treated with an enzymatic hydrolysate of phytic acid (hPA120) and gut bacteria. (2) Cell lines derived from healthy (NCM460D) and cancer (HCT116) colonic tissue and fecal bacteria from vegan (V) and omnivorous (O) donors were investigated. Fecal bacteria were grown in mucin and phytic acid supplemented medium. Cultured bacteria (BM) were loaded onto colonocytes alone (V BM and O BM) or in combination with the phytate hydrolysate (V BM + hPA120 and O BM + hPA120). After a treatment of 2 h, bacterial adhesion, secretion of cytokines, and the expression of genes and proteins important for immune response were determined. (3) All bacteria-treated colonocytes increased the expression of IL8 compared to controls. The significant increase of the secreted IL-8 (p < 0.01) in both cell lines was observed for O BM and O BM + hPA120. The increase of TNF, IL-1β, and IL-10 secretion in healthy colonocytes (V BM alone and with hPA120 treatments; p < 0.05) and for TNF and IL-10 in cancer cells (treatments except O BM + hPA120 and V BM, respectively; p > 0.05) were stated. A comparison of solely the effect of hPA120 on bacteria-treated colonocytes (BM vs. BM + hPA120) showed that hPA120 decreased expression of NFkB1 and TNFR (p < 0.001) in healthy colonocytes. In cancer colonocytes, the expression of TLR4 and IL1R increased after BM + hPA120 treatment, whereas the secretion of IL-8 and MYD88 and TNFR expression decreased (p < 0.01). (4) The investigated hPA120 showed a differentiated modulatory activity on the immune response of healthy and cancer human colonocytes. Especially when analyzed independently on the gut bacteria origin, it reduced the proinflammatory response of HCT116 cells to gut bacteria, while being neutral for the bacteria-treated healthy colonocytes.

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In vitro evaluation of the effect of the buckwheat protein hydrolysate on bacterial adhesion, physiology and cytokine secretion of Caco-2 cells

Abstract: Abstract

Cited by 13 publications

References 37 publications

Metabolism of strawberry mono- and dimeric ellagitannins in rats fed a diet containing fructo-oligosaccharides

Metabolism of strawberry mono- and dimeric ellagitannins in rats fed a diet containing fructo-oligosaccharides

Systematic Review of Human and Animal Evidence on the Role of Buckwheat Consumption on Gastrointestinal Health

Phytate Hydrolysate Differently Modulates the Immune Response of Human Healthy and Cancer Colonocytes to Intestinal Bacteria

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