In vitro evaluation of the antibacterial effects of intracanal micro plasma system treatment

Bufflier, Pascal; Suchett-Kaye, George; Morrier, Jean-Jacques; Benay, G.; Decoret, Dominique; Bonin, P; Renard, F.; Barsotti, Odile

doi:10.1016/s0099-2399(97)80202-6

Cited by 6 publications

(4 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The teeth were cleaned immediately after extraction to remove all attached hard and soft tissue and immersed in 5.25% sodium hypochlorite for 24 h to remove organic materials from the root surfaces (7,24–27). The teeth were stored in 0.9% sterile normal saline at room temperature until use (15,28). The crowns of all teeth were removed at the level of the CEJ, perpendicular to the long axis of the tooth with a rotary diamond disc and copious water spray, so that roots of similar length were obtained in all specimens (6,29–32).…”

Section: Methodsmentioning

confidence: 99%

Bacterial leakage of GuttaFlow‐filled root canals compared with Resilon/Epiphany and Gutta‐percha/AH26‐filled root canals

et al. 2010

View full text Add to dashboard Cite

The purpose of this study was to assess bacterial apical leakage in root canals obturated with GuttaFlow and compare this with the leakage of root canals obturated with Resilon/Epiphany or Gutta-percha/AH26. A total of 55 single-rooted human teeth were divided randomly into three experimental (n = 15) and two control groups (n = 5). Following a standardised preparation, the teeth were obturated with either GuttaFlow, Resilon/Epiphany or Gutta-percha/AH26. A two-chamber bacterial model using Enterococcus faecalis was employed to assess bacterial apical leakage for a period of 60 days. A Kruskal-Wallis test showed no significant differences between the seal of root canals obturated with GuttaFlow, Resilon/Ephiphany or Gutta-percha/AH26.

show abstract

Section: Methodsmentioning

confidence: 99%

Bacterial leakage of GuttaFlow‐filled root canals compared with Resilon/Epiphany and Gutta‐percha/AH26‐filled root canals

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The features supposed to be present in quintessential endodontic irrigants are superior antimicrobial effi cacy, non-cytotoxic, prudent towards the tissues of the periapical region, endotoxin inactivation, lubricating nature and breakdown of tissue. [7][8][9] Of all the irrigants used in endodontics, Sodium Hypochlorite(NaOCl) is considered as the gold standard. 7 The major disadvantage of NaOCl is that when near vital tissues, it irritates leading to severe infl ammatory reactions.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Antimicrobial Efficacy of the Different Concentrations of Chitosan against Staphylococcus epidermidis in Disinfection of the Root Canal System

Manali Nath,

Shashirekha Govind,

Amit Jena

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

View full text Add to dashboard Cite

During endodontic procedures, along with mechanical instrumentation, irrigants are imperative for pathogen eradication. As the strains of pathogens are becoming antibiotic resistant at a constant ascending pace and also the potentially harmful effect is associated with synthetic drugs, so studies on natural alternatives for irrigation are being done. Chitosan, a natural alternative, has been evaluated as an antimicrobial medicine. Recently, in cases presenting with persistent infections after endodontic treatment, it was noted that of all the microbial species identified, the prevalence of s.epidermidis was the most. In this current study, an analysis of the antimicrobial effectiveness of chitosan as a potent root canal irrigant, for disinfecting the root canal was conducted. Methodology: By the serial dilution method, the minimum inhibitory concentration was determined. Dilution of the test solution was done using 10% DMSO solution. After which, incubation in a rotary incubator, at 180 rotations per minute, the temperature of 37℃, for 24 hours was done. For obtaining the zones of inhibition of different concentration (0.2%, 0.5%, 0.75, 1%) of chitosan irrigant, the agar diffusion method was applied and the resultant clear zone was measured with a ruler. Result: Chitosan (liquid) had promising antibacterial activity against Staphylococcus epidermidis, with a 16 mm zone of inhibition at 100 µg/ml. Conclusion: Within the limitations of the present study, it was observed that, even at lower concentrations, chitosan (liquid form) effectively hindered the further growth of S.epidermidis.

show abstract

“…Irrigants have varying degrees of antimicrobial activity. The characteristics of an ideal endodontic irrigant includes good antimicrobial activity, non-cytotoxicity towards the surrounding periapical tissue, and the ability to inactivate endotoxins, to act as a lubricant, and to dissolve organic tissue remnants [3456]. The present study focused on the antimicrobial aspect of irrigants, as there are over 700 bacterial species in the oral cavity, with any individual harboring 100–200 species [7].…”

Section: Introductionmentioning

confidence: 99%

“…Sodium hypochlorite (NaOCl) has long been the gold-standard endodontic irrigant due to its good antimicrobial activity and tissue-dissolving capability [35]. However, it has been found to cause severe inflammatory reactions when placed in contact with vital tissues [8].…”

Section: Introductionmentioning

confidence: 99%

In vitroevaluation of octenidine as an antimicrobial agent againstStaphylococcus epidermidisin disinfecting the root canal system

et al. 2019

View full text Add to dashboard Cite

Objectives Irrigants are imperative in endodontic therapy for the elimination of pathogens from the infected root canal. The present study compared the antimicrobial efficacy of octenidine dihydrochloride (OCT) with chlorhexidine (CHX) and sodium hypochlorite (NaOCl) against Staphylococcus epidermidis ( S. epidermidis ) for root canal disinfection. Materials and Methods The minimum inhibitory concentration (MIC) was obtained using serial dilution method. The agar diffusion method was then used to determine the zones of inhibition for each irrigant. Lastly, forty 6-mm dentin blocks were prepared from human mandibular premolars and inoculated with S. epidermidis . Samples were randomly divided into 4 groups of 10 blocks and irrigated for 3 minutes with saline (control), 2% CHX, 3% NaOCl, or 0.1% OCT. Dentin samples were then collected immediately for microbial analysis, including an analysis of colony-forming units (CFUs). Results The MICs of each tested irrigant were 0.05% for CHX, 0.25% for NaOCl, and 0.0125% for OCT. All tested irrigants showed concentration-dependent increase in zones of inhibition, and 3% NaOCl showed the largest zone of inhibition amongst all tested irrigants ( p < 0.05). There were no significant differences among the CFU measurements of 2% CHX, 3% NaOCl, and 0.1% OCT showing complete elimination of S. epidermidis in all samples. Conclusions This study showed that OCT was comparable to or even more effective than CHX and NaOCl, demonstrating antimicrobial activity at low concentrations against S. epidermidis .

show abstract

In vitro evaluation of the antibacterial effects of intracanal micro plasma system treatment

Cited by 6 publications

References 10 publications

Bacterial leakage of GuttaFlow‐filled root canals compared with Resilon/Epiphany and Gutta‐percha/AH26‐filled root canals

Bacterial leakage of GuttaFlow‐filled root canals compared with Resilon/Epiphany and Gutta‐percha/AH26‐filled root canals

In Vitro Evaluation of Antimicrobial Efficacy of the Different Concentrations of Chitosan against Staphylococcus epidermidis in Disinfection of the Root Canal System

In vitroevaluation of octenidine as an antimicrobial agent againstStaphylococcus epidermidisin disinfecting the root canal system

Contact Info

Product

Resources

About