In vitro evaluation of trypanocidal activity in plants used in Argentine traditional medicine

Sülsen, Valeria P.; Güida, C.; Coussio, J.; Paveto, Cristina; Muschietti, Liliana; Martino, Virginia

doi:10.1007/s00436-005-0060-4

Cited by 42 publications

(26 citation statements)

References 21 publications

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“…Our findings corroborate previously reported data on trypanocidal activity of Lippia species polar extracts (Abe et al 2005;Sülsen et al 2006). Sülsen et al (2006) reported in vitro activity of an organic and an aqueous extracts obtained from leaves of Lippia integrifolia against epimastigote forms of T. cruzi, with 34.5 and 71.2% inhibition, respectively, at 10 µg mL -1 .…”

Section: Discussionsupporting

confidence: 93%

“…Sülsen et al (2006) reported in vitro activity of an organic and an aqueous extracts obtained from leaves of Lippia integrifolia against epimastigote forms of T. cruzi, with 34.5 and 71.2% inhibition, respectively, at 10 µg mL -1 . On the other hand, Abe et al (2005) reported in vitro activity of a methanol extract of Lippia dulcis leaves against trypomastigote forms of T. cruzi, with MIC (minimum inhibitory concentration) between 125 and 250 µg mL -1 .…”

Section: Discussionmentioning

confidence: 99%

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Oleanonic acid from Lippia lupulina (Verbenaceae) shows strong in vitro antileishmanial and antitrypanosomal activity

et al. 2016

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Leishmaniasis and Chagas disease affect millions of people in tropical and subtropical regions. Drugs used currently to treat such diseases often present undesirable side effects and low efficiency. The aim of this work was to identify extracts and isolated compounds from the genus Lippia with leishmanicidal and trypanocidal activity. , respectively). The triterpenoid oleanonic acid showed the strongest activity against these protozoans (IC 50 of 18.5 and 29.9 µM, respectively). Our results indicate that Lippia plants and their derivatives deserve further investigation in the search for new antiprotozoal drugs, particularly for the treatment of leishmaniasis and Chagas disease. KEYWORDS: Leishmanicidal, trypanocidal, triterpenoids, flavonoids, T. cruzi Ácido oleanônico isolado de Lippia lupulina (Verbenaceae) apresenta potentes atividades leishmanicida e tripanocida Leishmaniose e doença de Chagas afetam milhões de pessoas em regiões tropicais e subtropicais. As drogas atualmente usadas para tratar estas doenças frequentemente apresentam efeitos colaterais indesejáveis e baixa eficiência. Este trabalho teve como objetivo encontrar extratos, frações e compostos isolados de espécies do gênero Lippia com atividades leishmanicida e tripanocida. Quinze extratos de diferentes partes de plantas do gênero Lippia, com composições químicas parcialmente conhecidas, quatro frações de partição, seis substâncias e uma mistura de quatro flavanonas interconversíveis isolados de Lippia salviaefolia e Lippia lupulina foram testados, in vitro, frente a formas epimastigotas de Trypanosoma cruzi e promastigotas de Leishmania amazonensis. O extrato etanólico das raízes de L. lupulina apresentou atividade potente contra T. cruzi e L. amazonensis (IC 50 de 20,0 e 54,5 µg mL -1 , respectivamente), enquanto que o ácido oleanônico mostrou as atividades mais fortes contra estes protozoários, com IC 50 de 18,5 e 29,9 µM, respectivamente. Estes resultados indicam que espécies do gênero Lippia e seus derivados merecem investigações adicionais na busca por novas terapias antiprotozoárias, especialmente para o tratamento de leishmaniose e doença de Chagas.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Oleanonic acid from Lippia lupulina (Verbenaceae) shows strong in vitro antileishmanial and antitrypanosomal activity

et al. 2016

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“…The leishmanicidal and trypanocidal activity of essential oils from Lippia spp have been already demonstrated in some studies (Sulsen et al, 2006;Escobar et al, 2010 …”

Section: Discussionmentioning

confidence: 92%

Leishmanicidal activity of carvacrol-rich essential oil from Lippia sidoides Cham

et al. 2012

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Leishamaniasis is a disease that aff ects more than 2 million people worldwide, whose causative agent is Leishmania spp. The current therapy for leishmaniasis is far from satisfactory. All available drugs, including pentavalent antimony, require parenteral administration and are potentially toxic. Moreover, an increase in clinical resistance to these drugs has been reported. In this scenario, plant essential oils used traditionally in folk medicine are emerging as alternative sources for chemotherapeutic compounds. In this study, in vitro leishmanicidal eff ects of a thymol-and a carvacrol-rich essential oil from leaves of Lippia sidoides Cham. were investigated. The essential oils were extracted and their constituents were characterized by gas chromatography coupled to mass spectrometry (GC/MS). Both essential oils showed signifi cant activity against promastigote forms of Leishmania chagasi. However, we found that carvacrol-rich essential oil was more eff ective, with IC 50 /72 h of 54.8 μg/mL compared to 74.1 μg/mL for thymol-rich oil. Carvacrol also showed lower IC 50 than thymol. Our data suggest that L. sidoides essential oils are indeed promising sources of leishmanicidal compounds.

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“…Antioxidant, trypanocidal and antibacterial activities for this species have also been reported. [7][8][9] …”

Section: Introductionmentioning

confidence: 99%

Volatile constituents of five Baccharis Species from Northeastern Argentina

Retta¹,

Gattuso²,

Gattuso³

et al. 2009

J. Braz. Chem. Soc.

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Os óleos essenciais obtidos por hidrodestilação das partes aéreas de Baccharis gaudichaudiana, B. microcephala, B. penningtonii, B. phyteumoides e B. spicata coletadas em diferentes regiões da Argentina foram analisados qualitativa e quantitativamente utilizando um sistema CG e CG/EM com uma configuração especial. Os rendimentos dos óleos variaram de 0,1 a 0,2% (m/v de planta seca). Diferentes compostos foram observados na composição dos óleos essenciais das espécies estudadas: B. gaudichaudiana apresenta espatulenol e β-pineno como constituintes principais e, B. microcephala e B. spicata, espatulenol e óxido de cariofileno em concentrações mais elevadas. Na última espécie citada, o β-pineno também foi encontrado em quantidades significativas. Germacreno D foi o principal componente presente na B. penningtonii. Finalmente, β-selineno e acetato de fitol E foram os principais componentes encontrados na B. phyteumoides.Essential oils obtained by hydrodistillation from the aerial parts of Baccharis gaudichaudiana, B. microcephala, B. penningtonii, B. phyteumoides and B. spicata collected from different locations in Argentina were analyzed. Qualitative and quantitative analysis were performed using a GC/FID/MS system with a special configuration. Essential oil yields ranged from 0.1 to 0.2% (m/v of dry material). Different chemical patterns were observed in the essential oils composition of the species studied: spathulenol and β-pinene were the main constituents in B. gaudichaudiana, whereas caryophyllene oxide and spathulenol were the major constituents in B. microcephala and B. spicata. In this latter species, β-pinene has also been found in high amounts. Germacrene D was the major compound in B. penningtonii. Finally, β-selinene and phytol acetate E were the main components in B. phyteumoides. Keywords: Baccharis gaudichaudiana, B. microcephala, B. penningtonii, B. phyteumoides, B. spicata, carqueja, essential oils IntroductionThe genus Baccharis belongs to the Asteraceae family. This genus comprises more than 400 species in America, mainly South America. 1 Essential oils from 38 species of this genus have previously been analyzed, accounting in many cases for the value of these plants in traditional medicine as diuretics and stomachics and in fragrance industry. Because of their bitter properties these oils are also frequently used to prepare alcoholic and non-alcoholic beverages. Ninety six species of Baccharis were found in Argentina, and they were classified into 15 sections. Section Caulopterae or Alatae comprises 10 species, all having stems with 2 or 3 longitudinal lateral wings. 2,3 The most common Baccharis species used in traditional medicine and phytotherapy of southern Brazil, Paraguay, Uruguay and Argentina belong to this section: e.g. B. trimera, B. articulata and B. crispa. Among the remaining species of this section, B. phyteumoides, B. penningtonii. B. microcephala and B. gaudichaudiana, were selected to be studied. B. spicata was also analyzed. All these aromatic species are commonly named "carqu...

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In vitro evaluation of trypanocidal activity in plants used in Argentine traditional medicine

Cited by 42 publications

References 21 publications

Oleanonic acid from Lippia lupulina (Verbenaceae) shows strong in vitro antileishmanial and antitrypanosomal activity

Oleanonic acid from Lippia lupulina (Verbenaceae) shows strong in vitro antileishmanial and antitrypanosomal activity

Leishmanicidal activity of carvacrol-rich essential oil from Lippia sidoides Cham

Volatile constituents of five Baccharis Species from Northeastern Argentina

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