In vitro extracellular matrix deposition by vascular smooth muscle cells grown in fibroin scaffolds, and the regulation of TGF-β1

Song, Guangzhou; Zheng, Chang-dong; Liu, Yunfei; Ding, Mengyao; Liu, Ping; Xu, Jianzhong; Wang, Jiannan

doi:10.1016/j.matdes.2020.109428

Cited by 12 publications

(4 citation statements)

References 45 publications

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“…Also, the model wanted to achieve the intimal thickening only by adding VSMCs in the collagen. Atherosclerosis intima thickening requires VSMCs proliferation and matrix protein deposition in the intima, [ 40 ] which was not possible by 24 h incubation time [ 41 ] and was overlooked by this model.…”

Section: Introductionmentioning

confidence: 99%

Modeling Foam Cell Formation in A Hydrogel‐Based 3D‐Intimal Model: A Study of The Role of Multi‐Diseases During Early Atherosclerosis

Akther,

Sajin,

Moonshi

et al. 2024

Advanced Biology

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Monocyte recruitment and transmigration are crucial in atherosclerotic plaque development. The multi‐disease complexities aggravate the situation and continue to be a constant concern for understanding atherosclerosis plaque development. Herein, a 3D hydrogel‐based model that integrates disease‐induced microenvironments is sought to be designed, allowing us to explore the early stages of atherosclerosis, specifically examining monocyte fate in multi‐disease complexities. As a proof‐of‐concept study, murine cells are employed to develop the model. The model is constructed with collagen embedded with murine aortic smooth muscle cells and a murine endothelial monolayer lining. The model achieves in vitro disease complexities using external stimuli such as glucose and lipopolysaccharide (LPS). Hyperglycemia exhibits a significant increase in monocyte adhesion but no enhancement in monocyte transmigration and foam cell conversion compared to euglycemia. Chronic infection achieved by LPS stimulation results in a remarkable augment in initial monocyte attachment and a significant increment in monocyte transmigration and foam cells in all concentrations. Moreover, the model exhibits synergistic sensitivity under multi‐disease conditions such as hyperglycemia and infection, enhancing initial monocyte attachment, cell transmigration, and foam cell formation. Additionally, western blot data prove the enhanced levels of inflammatory biomarkers, indicating the model's capability to mimic disease‐induced complexities during early atherosclerosis progression.

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Section: Introductionmentioning

confidence: 99%

Modeling Foam Cell Formation in A Hydrogel‐Based 3D‐Intimal Model: A Study of The Role of Multi‐Diseases During Early Atherosclerosis

Akther,

Sajin,

Moonshi

et al. 2024

Advanced Biology

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“…Recently, the concept of tissue engineering has been incorporated into periodontal regenerative therapy [1,2], whereby cells [3][4][5], signaling molecules (growth factors) [6,7], and scaffold materials [8,9] are jointly used for tissue regeneration. Scaffolds play an important role in promoting cell adhesion, proliferation, differentiation, and subsequent extracellular matrix deposition and blood vessel network establishment in tissue defects [10,11]. In addition, due to their mechanical properties, scaffolds maintain the regenerative space and store growth and nutritional factors [12].…”

Section: Introductionmentioning

confidence: 99%

Periodontal Tissue Regeneration by Recombinant Human Collagen Peptide Granules Applied With β-Tricalcium Phosphate Fine Particles

Yoshino¹,

Miyaji²,

Nishida³

et al. 2022

Preprint

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Recombinant human collagen peptide (RCP) is a recombinantly created xeno-free biomaterial enriched in RGD (arginine-glycine-aspartic acid) sequences, with good processability that is being investigated for regenerative medicine applications. Recently, the biocompatibility and osteogenic ability of β-TCP/RCP (RCP granules combined with β-tricalcium phosphate (TCP) submicron particles) were demonstrated. In the present study, β-TCP/RCP was implanted into experimental periodontal tissue defects (three-walled bone defect) created in beagle dogs to investigate tissue responses and subsequent regenerative effects. Micro computed tomography image analysis at 8 weeks postoperatively showed that the amount of new bone after β-TCP/RCP graft was significantly greater (2.2 fold, P&lt;0.05) than that of the control (no graft) group. Histological findings showed that the transplanted β-TCP/RCP induced active bone-like tissue formation including TRAP-positive and OCN-positive cells as well as bioabsorbability. Ankylosis did not occur, and periostin-positive periodontal ligament-like tissue formation was observed. Histological measurements revealed that β-TCP/RCP implantation formed 1.7-fold more bone-like tissue and 2.1-fold more periodontal ligament-like tissue than the control, and significantly suppressed gingival recession and epithelial downgrowth (P&lt;0.05). These results suggest that β-TCP/RCP is effective as a periodontal tissue regenerative material.

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“…However, non-crosslinking (meth) acrylated HA has poor rheological properties that are difficult to be extruded filamentarily, thus resulting in poor printability . This limitation can be overcome by crosslinking (meth) acrylated HA with other natural polymers, for example, gelatin and chitosan, or synthetic polymers, for example, poly(glycidol), to improve its rheological properties suitable for bioprinting. ,, However, for avoidance of the potential risk for immune response from these additive exogenous polymers, pure HA hydrogel constructs have been already developed by mixtures of (meth) acrylated HA with other HA derivatives based on physical crosslinking mechanisms including guest–host supramolecular assembly and thermoresponsive crosslinking and secondary photoinduced covalent crosslinking.…”

Section: Introductionmentioning

confidence: 99%

“…18 This limitation can be overcome by crosslinking (meth) acrylated HA with other natural polymers, for example, gelatin and chitosan, or synthetic polymers, for example, poly(glycidol), to improve its rheological properties suitable for bioprinting. 11,17,19 However, for avoidance of the potential risk for immune response from these additive exogenous polymers, 20 molecular assembly 21 and thermoresponsive crosslinking 22 and secondary photoinduced covalent crosslinking. In this work, stepwise multiple crosslinking strategy is proposed for pure HA hydrogel precursor for the fabrication of 3D constructs via extrusion-based 3D printing.…”

Section: Introductionmentioning

confidence: 99%

Multiple Crosslinking Hyaluronic Acid Hydrogels with Improved Strength and 3D Printability

Wan

Fan

Zhang

et al. 2021

ACS Appl. Bio Mater.

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Hyaluronic acid (HA) hydrogel is preferred for biomedicine applications, as it possesses biodegradability, biocompatibility, and cell-regulated capacity as well as high hydration nature similar to the native extracellular matrix. However, HA hydrogel fabricated via a 3D printing technique often faces poor printing properties. In this study, maleiated sodium hyaluronate (MHA) with a high substituted degree of the acrylate group (i.e., 2.27) and thiolated sodium hyaluronate (SHHA) were synthesized. By blending these modified HAs, the MHA/SHHA hydrogels were prepared via pre-crosslinking through thiol–acrylate Michael addition and subsequently covalent crosslinking using thiol–acrylate and acrylate–acrylate photopolymerization mechanisms. Rheological properties, swelling behaviors, and mechanical properties can be modulated by altering the molar ratio of the thiol group and acrylate group. The results showed that the MHA/SHHA hydrogel precursors have rapidly gelling capacity and improved compressive strength. Based on these results, high-resolution hydrogel scaffolds with good structural stability were prepared by extrusion-based 3D printing. This HA hydrogel is cytocompatible and capable of supporting adherence of L929 cells, indicating its great potential for tissue engineering scaffolds.

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In vitro extracellular matrix deposition by vascular smooth muscle cells grown in fibroin scaffolds, and the regulation of TGF-β1

Cited by 12 publications

References 45 publications

Modeling Foam Cell Formation in A Hydrogel‐Based 3D‐Intimal Model: A Study of The Role of Multi‐Diseases During Early Atherosclerosis

Modeling Foam Cell Formation in A Hydrogel‐Based 3D‐Intimal Model: A Study of The Role of Multi‐Diseases During Early Atherosclerosis

Periodontal Tissue Regeneration by Recombinant Human Collagen Peptide Granules Applied With β-Tricalcium Phosphate Fine Particles

Multiple Crosslinking Hyaluronic Acid Hydrogels with Improved Strength and 3D Printability

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