In vitro fermentation of Bangia fusco-purpurea polysaccharide by human gut microbiota and the protective effects of the resultant products on Caco-2 cells from lipopolysaccharide-induced injury

In vitro-simulated intestinal flora fermentation of Porphyra haitanensis polysaccharides obtained by different assisted extractions and their fermented products against HT-29 human colon cancer cells

“…25 We found that Bangia fuscopurpurea polysaccharides were degraded at transverse colon fermentation for 18 h (ref. 6) and carrageenan was degraded at transverse colon fermentation for 36 h, 24 while PHPs were completed at ascending colon fermentation for 18 h. Thus, the results suggest that different types of red alga polysaccharides can be utilized and degraded differently by the intestinal flora.…”

“…The in vitro ascending-transverse-descending colon fermentation was simulated by human intestinal flora. 6 PHPs obtained by different assisted extractions were added to basal nutrient medium (BNM) at a material-liquid ratio (m/V) of 1 : 100 and pasteurized at 65 °C for 1 h. Meanwhile, BNM without PHPs was used as the control (CON). The activated human intestinal flora (strain : BHI medium = 1 : 100) was mixed with BNM containing PHPs in a ratio of 1 : 1 (v/v), and adjusted pH to 5.7 to simulate ascending colon fermentation for 18 h (i.e., V1-18 stage).…”

“…SCFAs, such as butyric acid, acetic acid and propionic acid, can induce the production of ROS and modulate the metabolism and transcriptomic levels, exhibiting anticancer activity in colon cancer cells and animal models. 6,7 In addition, studies have demonstrated that polysaccharide-rich extracts can provide in vivo and in vitro anti-colon-cancer effects by inducing the inhibition of PI3K/AKT 8 and tumor cell apoptosis and regulating the intestinal flora. 9 Porphyra haitanensis is an important economic red alga in China, which is mainly distributed in the Zhejiang, Fujian and Guangdong coast.…”

In vitro fermentation of seaweed polysaccharides and tea polyphenol blends by human intestinal flora and their effects on intestinal inflammation

“…Two peaks at 13.80 min (with M w of 1389.06 kDa) and 14.51 min (with M w of 757.87 kDa) were found in ι-carrageenan, and the degradation of ι-carrageenan was also at transverse colon fermentation for 36 h. Our results were well consistent with previous studies, and the red seaweed polysaccharides can be degraded and utilized by intestinal flora in the colon. 31–33…”

“…Two peaks at 13.80 min (with M w of 1389.06 kDa) and 14.51 min (with M w of 757.87 kDa) were found in ι-carrageenan, and the degradation of ι-carrageenan was also at transverse colon fermentation for 36 h. Our results were well consistent with previous studies, and the red seaweed polysaccharides can be degraded and utilized by intestinal flora in the colon. [31][32][33] 3.3. Effect of seaweed polysaccharides and tea polyphenol blend fermentation on SCFAs content SCFAs result from complex interactions between carbohydrates and the gut microbiota in the intestine, which represent the major flow of carbon in diet through the microbiome to the host.…”