In Vitro Fermentation of Porcine Milk Oligosaccharides and Galacto-oligosaccharides Using Piglet Fecal Inoculum

Difilippo, Elisabetta; Pan, Feipeng; Logtenberg, Madelon J; Willems, Rianne H A M; Braber, Saskia; Fink‐Gremmels, Johanna; Schols, Henk A.; Gruppen, Harry

doi:10.1021/acs.jafc.5b05384

Cited by 25 publications

(19 citation statements)

References 37 publications

(107 reference statements)

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“…Another promising alternative to antibiotics in animal feed is using probiotics to improve the absorption of nutrients [ 47 ]. During the initial development of mammalian neonates, breast milk is a nutritious food and a natural reservoir of probiotics [ 48 , 49 ] that satisfies neonatal needs [ 50 ]. In the present work, we focused on the sow milk microbiota using culturomics.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets

et al. 2022

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Background In modern animal husbandry, breeders pay increasing attention to improving sow nutrition during pregnancy and lactation to favor the health of neonates. Sow milk is a main food source for piglets during their first three weeks of life, which is not only a rich repository of essential nutrients and a broad range of bioactive compounds, but also an indispensable source of commensal bacteria. Maternal milk microorganisms are important sources of commensal bacteria for the neonatal gut. Bacteria from maternal milk may confer a health benefit on the host. Methods Sow milk bacteria were isolated using culturomics followed by identification using 16S rRNA gene sequencing. To screen isolates for potential probiotic activity, the functional evaluation was conducted to assess their antagonistic activity against pathogens in vitro and evaluate their resistance against oxidative stress in damaged Drosophila induced by paraquat. In a piglet feeding trial, a total of 54 newborn suckling piglets were chosen from nine sows and randomly assigned to three treatments with different concentrations of a candidate strain. Multiple approaches were carried out to verify its antioxidant function including western blotting, enzyme activity analysis, metabolomics and 16S rRNA gene amplicon sequencing. Results The 1240 isolates were screened out from the sow milk microbiota and grouped into 271 bacterial taxa based on a nonredundant set of 16S rRNA gene sequencing. Among 80 Pediococcus isolates, a new Pediococcus pentosaceus strain (SMM914) showed the best performance in inhibition ability against swine pathogens and in a Drosophila model challenged by paraquat. Pretreatment of piglets with SMM914 induced the Nrf2-Keap1 antioxidant signaling pathway and greatly affected the pathways of amino acid metabolism and lipid metabolism in plasma. In the colon, the relative abundance of Lactobacillus was significantly increased in the high dose SMM914 group compared with the control group. Conclusion P. pentosaceus SMM914 is a promising probiotic conferring antioxidant capacity by activating the Nrf2-Keap1 antioxidant signaling pathway in piglets. Our study provided useful resources for better understanding the relationships between the maternal microbiota and offspring.

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Section: Discussionmentioning

confidence: 99%

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets

et al. 2022

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“…Sow’s milk contains not only lactose but at least 119 PMOs ( 34 ), which are composed of the monosaccharides fucose, galactose, glucose, N -acetylglucosamine, N -acetylgalactosamine, and sialic acid bound to a lactose or N -acetyllactosamine core ( 35 ). These PMOs are generally resistant to host digestive enzymes in the small intestine and are instead fermented by the colonic microbiome into SCFAs ( 36 , 37 ).…”

Section: Discussionmentioning

confidence: 99%

Weaning Age and Its Effect on the Development of the Swine Gut Microbiome and Resistome

et al. 2021

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“…Porcine milk stimulates fermentation and thus increases SCFA and certain MCFA in the gastrointestinal tract of piglets due to their large amounts of disaccharides and oligosaccharides [53]. The lactose in the milk promotes lactate fermentation, commencing in piglet's stomach, whereas milk oligosaccharides are important for early SCFA production [54].…”

Section: Lipid Composition Of Porcine Colostrum and Milkmentioning

confidence: 99%

The Role of Dietary and Microbial Fatty Acids in the Control of Inflammation in Neonatal Piglets

Metzler‐Zebeli

2021

Animals

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Excessive inflammation and a reduced gut mucosal barrier are major causes for gut dysfunction in piglets. The fatty acid (FA) composition of the membrane lipids is crucial for mediating inflammatory signalling and is largely determined by their dietary intake. Porcine colostrum and milk are the major sources of fat in neonatal piglets. Both are rich in fat, demonstrating the dependence of the young metabolism from fat and providing the young organism with the optimum profile of lipids for growth and development. The manipulation of sow’s dietary polyunsaturated FA (PUFA) intake has been shown to be an efficient strategy to increase the transfer of specific FAs to the piglet for incorporation in enteric tissues and cell membranes. n-3 PUFAs, especially seems to be beneficial for the immune response and gut epithelial barrier function, supporting the piglet’s enteric defences in situations of increased stress such as weaning. Little is known about microbial lipid mediators and their role in gut barrier function and inhibition of inflammation in neonatal piglets. The present review summarizes the current knowledge of lipid nutrition in new-born piglets, comparing the FA ingestion from milk and plant-based lipid sources and touching the areas of host lipid signalling, inflammatory signalling and microbially derived FAs.

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In Vitro Fermentation of Porcine Milk Oligosaccharides and Galacto-oligosaccharides Using Piglet Fecal Inoculum

Cited by 25 publications

References 37 publications

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets

Weaning Age and Its Effect on the Development of the Swine Gut Microbiome and Resistome

The Role of Dietary and Microbial Fatty Acids in the Control of Inflammation in Neonatal Piglets

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