In vitro fertility evaluation of cryopreserved ram semen and its correlation with relative in vivo fertility

Choudhry, T.M.; Berger, Trish; Dally, Martin R.

doi:10.1016/0093-691x(95)00091-l

Cited by 22 publications

(8 citation statements)

References 5 publications

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“…Attempts to correlate the outcomes of sperm functional tests with the fertility of FT ram semen have given contradictory findings. Choudhry et al. (1995) reported that sperm penetration assay (SPA) using zona‐free hamster oocytes might be a useful assay for predicting the fertility of FT ram semen used in laparoscopic intrauterine insemination.…”

Section: Introductionmentioning

confidence: 99%

Effect of Ram Age on Structural and Functional Competence of Frozen-Thawed Spermatozoa in Dairy Sheep

Lymberopoulos

Tsakmakidis

Khalifa

2008

Reproduction in Domestic Animals

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The objectives of this study were to investigate the influence of ram age on structural and functional competence of frozen-thawed spermatozoa and to test the hypothesis that increasing number of sperm bound to the zona pellucida in vitro was associated with decreasing in vivo fertility of frozen semen. Rams were allocated into two groups. Each group consisted of five rams aged either 1-2 years (young) or 4-5 years (mature). Three successive ejaculates were collected from each ram using an artificial vagina. Only ejaculates of >or= 2.5 x 10(9) sperm/ml and 80% sperm progressive motility were pooled per ram, diluted with Bioxcell medium and frozen in 0.25 ml straws. The end points of post-thawing semen evaluation were computer-assisted cell motility analysis, sperm capacitation (chlortetracycline assay), simultaneous assessment of plasma membrane integrity, mitochondrial membrane potential and condensation status of nucleus, per-cell analysis of lipid peroxidation using C11-BODIPY(581/591), sperm-hemizona binding (HZB) ability and sperm fertility after laparoscopic insemination of ewes (n = 114) in the progestagen-synchronized oestrus. The results showed that mature rams had significantly lower values of sperm hyperactivated motility and peroxidized sperm, higher percentages of live non-capacitated sperm and sperm cells with intact plasma membrane, functional mitochondria and condensed chromatin, as well as, greater lambing rate and ewe prolificacy. Sperm HZB binding ability was higher (p < 0.05) for young than for mature rams. Significant correlations were found between number of spermatozoa bound to the zona pellucida and semen fertility (r = -0.63 to -0.71). In conclusion, mature rams have better semen quality and in vivo fertility than young rams. Cryocapacitation can be involved in decreasing ram semen fertility as evidenced by the high number of spermatozoa bound to the zona pellucida in vitro.

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Section: Introductionmentioning

confidence: 99%

Effect of Ram Age on Structural and Functional Competence of Frozen-Thawed Spermatozoa in Dairy Sheep

Lymberopoulos

Tsakmakidis

Khalifa

2008

Reproduction in Domestic Animals

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“…Recently, sperm function was evaluated in an endangered African antelope, the scimitar‐horned oryx ( Oryx dammath ), by means of a heterologous IVF system with zona‐intact domestic cow oocytes (Roth et al, 1998; 1999). Human sperm function is assessed by the sperm penetration assay (SPA) with zona‐free hamster oocytes (Yanagimachi, 1984), and also has been used in several domestic species (Davis et al, 1987; Berger and Parker, 1989; Hammitt et al, 1989; Choudhry et al, 1995; Berger et al, 1996) and wild species (Lambert et al, 1991). Standardized SPA protocols are highly successful (Berger and Parker, 1989), and, if deer sperm are capable of interacting with hamster oocytes, this heterologous IVF could offer a valuable method of studying sperm functionality in this species.…”

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confidence: 99%

Relationship Between the Characteristics of Epididymal Red Deer Spermatozoa and Penetrability Into Zona‐Free Hamster Ova

Soler¹,

Garde

2003

Journal of Andrology

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A heterologous (zona-free hamster oocytes) in vitro fertilization (IVF) system was used to evaluate the relationship between sperm factors and penetration capacity of epididymal red deer spermatozoa. The sperm parameters evaluated in 36 sperm samples obtained postmortem from stags selectively shot during the rutting season were sperm motility, functional integrity of plasma membrane by means of the hypo-osmotic swelling test (HOST), and, simultaneously, viability and acrosomal status via a triple-stain technique. Zona-free hamster oocytes were used to evaluate the capacity of the different sperm assays to predict in vitro penetration. In order to increase the variability in sperm quality, we recovered samples from stags at different intervals between the death of the male and the collection of the genitalia. All measures of sperm quality declined progressively (P <.001) with increasing intervals between death and sample collection. In addition, many sperm parameters were related to penetration ability in vitro. Subsequently, sperm samples were rearranged in 2 categories according to the interval that had elapsed between death and the collection of the genitalia (group 1, short interval = 0-12 h; group 2, large interval = 18-40 hours). When samples were grouped, less correlation achieved significance, especially for group 1, than when samples were not divided. Also, correlation between the number of sperm per oocyte and sperm parameters for group 1, which had the highest values of sperm quality, failed to reach significance. It is concluded that the classical parameters accepted in assessing the viability of deer spermatozoa can be good predictors of the penetrating ability of the spermatozoa when satisfactory in vitro conditions are used for the development of the IVF system. Also, this study demonstrates that compatible heterologous gamete interaction allows thorough assessment of sperm function in a wild deer.

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“…For a preliminary approach, heterologous fertilization was chosen to test the fertilizing ability of ram semen. Several authors reported heterologous fertilization as a useful tool for predicting the fertility of frozen‐thawed ram semen either in vivo or in vitro because a high relationship was showed between heterologous IVF and in vivo male fertility as well as embryo production rates (Choudhry et al. 1995; Garcia‐Alvarez et al.…”

Section: Discussionmentioning

confidence: 99%

The Prion‐like Protein Doppel Enhances Ovine Spermatozoa Fertilizing Ability

Pimenta¹,

Dias²,

Marques³

et al. 2011

Reprod Domestic Animals

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The function of prion-like protein Doppel was suggested to be related to male fertility. In this study, the importance of ovine Doppel polypeptide on spermatozoa capacitation and fertilization was evaluated. After refolding, recombinant Doppel (rDpl) was supplemented with different concentrations (40, 80 or 190 ng/ml) to ovine spermatozoa during the capacitation process. In experiment 1, post-thawed ovine spermatozoa were incubated with different concentrations of rDpl during 1 h for swim-up, and changes in sperm motility, concentration, vigour, viability and capacitation were monitored (10 replicates). In experiment 2, the fertilization ability of post-swim-up spermatozoa incubated as above was tested through heterologous fertilization of bovine in vitro matured oocytes (n = 423, three replicates). Regardless of dosage, rDpl improved (p ≤ 0.03) spermatozoa viability. Sperm individual motility and vigour were the highest (p ≤ 0.04) for the group receiving 190 ng/ml rDpl. Sperm supplemented with the highest doses of rDpl achieved higher (p = 0.02) fertilization rates (56.0 ± 3.0%) than control (39.1 ± 2.2%) and 40 ng/ml rDpl (39.8 ± 2.7%). Preliminary data suggest that Doppel protein may enhance in vitro spermatozoa fertilizing ability.

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In vitro fertility evaluation of cryopreserved ram semen and its correlation with relative in vivo fertility

Cited by 22 publications

References 5 publications

Effect of Ram Age on Structural and Functional Competence of Frozen-Thawed Spermatozoa in Dairy Sheep

Effect of Ram Age on Structural and Functional Competence of Frozen-Thawed Spermatozoa in Dairy Sheep

Relationship Between the Characteristics of Epididymal Red Deer Spermatozoa and Penetrability Into Zona‐Free Hamster Ova

The Prion‐like Protein Doppel Enhances Ovine Spermatozoa Fertilizing Ability

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