In vitro fertilization of ova from cows experimentally infected with a non-cytopathic strain of bovine viral diarrhea virus

Bielański, A.; Dubuc, C.

doi:10.1016/0378-4320(95)98107-f

Cited by 46 publications

(13 citation statements)

References 12 publications

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“…Can an EIAV-infected embryo develop into an exportable blastocyst? As indicated by studies in other species [46], the proportion of embryos that developed to the blastocyst stage was significantly higher for the control group (29%, 18/62) than for the infected group (13.8%, 17/123). Stringfellow et al .…”

Section: Methodsmentioning

confidence: 57%

“…Organisms often contaminate follicular fluid, granulosa, and cumulus cells and bind to the ZP (hence are external to the oocyte) before penetrating into the oocyte during fertilization [44]. It seems, therefore, that techniques for removing cells surrounding the oocyte as well as in vitro maturation at 39 °C and washing of the oocyte could account for virus inactivation or removal [46]. Using this principle, Terriou et al [40] confirmed that oocyte washing procedure was sufficient to remove HIV from the oocytes, which in turn minimized the risk of embryo infection during in vitro manipulation.…”

Section: Discussionmentioning

confidence: 99%

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The risk of introduction of equine infectious anemia virus into USA via cloned horse embryos imported from Canada

et al. 2012

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Deriving horse oocytes in the USA is hampered by the lack of abattoirs processing horse carcasses which could provide abundant quantities of ovaries from slaughtered mares. Therefore, several cloning industries in the USA are attempting to import cloned horse embryos from Canada. Like any agricultural commodity, cloned embryos pose a risk of introduction of exotic animal diseases into the importing country. Under such circumstances, risk assessment could provide an objective, transparent, and internationally accepted means for evaluating the risk. This quantitative risk assessment (QRA) was initiated to determine the risk of introduction of Equine infectious anemia virus (EIAV) into USA via cloned horse embryos imported from Canada. In assessing the risk, a structured knowledge base regarding cloning in relation to EIA was first developed. Based on the knowledge base, a scenario tree was developed to determine conditions (with mathematical probabilities) that could lead to the introduction and maintenance of EIAV along the cloning pathway. Parameters for the occurrence of the event at each node were estimated using published literature. Using @RISK software and setting Monte Carlo simulation at 50 000 iterations, the probability of importing an EIAV-infected cloned horse embryo was 1.8×10−9 (R = 1.5×10−12 to 2.9×10−8). Taking into account the current protocol for equine cloning and assuming the yield of 5 to 30 clones per year, the possible number of EIAV-infected cloned horse embryos ranged from 2.0×10−10 to 9.1×10−5 (Mean = 1.4×10−6) per year. Consequently, it would take up to 1.5×107 (R = 1.6×104 to 5.1×1010) years for EIAV to be introduced into the USA. Based on the knowledge base and our critical pathway analysis, the biological plausibility of introducing EIAV into USA via cloned horse embryos imported from Canada is extremely low.

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Section: Methodsmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

The risk of introduction of equine infectious anemia virus into USA via cloned horse embryos imported from Canada

et al. 2012

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“…The ZP acts as a large molecular weight sieve and prevents infection by many types of viruses (33). The BVDV indicated that embryos produced using in vitro maturation and IVF from infected cattle (whose FF and cumulus-oocyte complexes were positive for virus) remained negative for the virus after at least 7 days (34). There are many more studies where bovine embryos were exposed to several types of viruses (acabane virus, bovine leukemia virus, bluetongue virus, BVDV, and foot-and-mouth disease virus) and the pathogen was not detected on the embryo (22,23,(35)(36)(37)(38).…”

Section: Embryosmentioning

confidence: 99%

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Pomeroy

Harris

Conaghan

et al. 2010

Fertility and Sterility

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“…Moreover, conflicting results were obtained with oocytes originating from infected donors. Some studies confirmed the insufficient effects of washing on IVF embryos infected with BHV-1 [9], BVDV [66], whereas no evidence of pathogen after washing was observed with BVDV [8]. The discrepancies between these results raise doubts on the transposition of in vitro contamination hardjo in the pores, matrix and channels of ZP and in the embryonic cells, indicating the ability of these pathogenic agents to attach the ZP or penetrate into the embryos.…”

Section: Risks Of Disease Transmission Via Contaminated Ivf Embryosmentioning

confidence: 99%

Risks of transmissible diseases in relation to embryo transfer

et al. 2001

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In vitro fertilization of ova from cows experimentally infected with a non-cytopathic strain of bovine viral diarrhea virus

Cited by 46 publications

References 12 publications

The risk of introduction of equine infectious anemia virus into USA via cloned horse embryos imported from Canada

The risk of introduction of equine infectious anemia virus into USA via cloned horse embryos imported from Canada

Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk

Risks of transmissible diseases in relation to embryo transfer

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