In vitro Fertilization with Isolated, Single Gametes Results in Zygotic Embryogenesis and Fertile Maize Plants

Kranz, Erhard; Lörz, Horst

doi:10.2307/3869611

Cited by 46 publications

(29 citation statements)

References 29 publications

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“…Egg cells and sperm cells were isolated from ears and pollen of this line as described by Kranz and LoÈ rz (1993). Electrofusion (in vitro fertilisation) of single gametes and cultivation of in vitro zygotes was performed according to methods described in the same paper.…”

Section: Plant Material Gamete Isolation and In Vitro Fertilisationmentioning

confidence: 99%

“…The cells were collected and stored at A80°C until usage. After gamete fusion, in vitro zygotes were cultivated for 18 h without feeder cells in the medium described by Kranz and LoÈ rz (1993) to avoid RNA contamination from lysed feeder cells.…”

Section: Plant Material Gamete Isolation and In Vitro Fertilisationmentioning

confidence: 99%

“…In maize, it is possible to isolate gametes, fuse them in vitro and regenerate normal and fertile plants from the zygotes thus formed (Kranz and LoÈ rz 1993). The time course of early events in zygote development has been determined (reviewed by Kranz and Dresselhaus 1996), and with the advent of novel and sensitive molecular methods (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Novel ribosomal genes from maize are differentially expressed in the zygotic and somatic cell cycles

Dresselhaus¹,

Cordts²,

Heuer³

et al. 1999

Mol Gen Genet

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We have isolated cDNAs representing more than 50 different genes from libraries of unfertilised egg cells and zygotes of maize, expression of which is up- or downregulated after in vitro fertilisation (IVF). Among the cDNAs isolated are seven which encode proteins that are probably involved in translation and two encoding proteins probably involved in DNA replication. The latter genes are strongly induced on fertilisation. This indicates that zygotic gene activation (ZGA) - the switch from maternal to embryonic control of development - occurs in the zygote shortly after fertilisation in higher plants - earlier than in animal systems so far investigated. Four novel transcripts for ribosomal proteins (S21A, S21B, L39, P0) involved in ribosome biosynthesis and translation were analysed in more detail. The expression of all four genes correlates with cell division activity and is strongly induced during the G1 phase of the somatic cell cycle. A different mode of regulation operates in the first embryonic cell cycle: relatively large amounts of transcript are stored in the unfertilised egg cells, and by 18 h after IVF, two ribosomal genes are induced while a third is downregulated. These results indicate that using the combination of single-cell culture techniques with novel molecular methods, it is possible to isolate and study numerous genes expressed in female gametes and zygotes of higher plants. The detailed analysis of the four ribosomal protein genes demonstrates that the zygotic and somatic cell cycles are differentially regulated.

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Section: Plant Material Gamete Isolation and In Vitro Fertilisationmentioning

confidence: 99%

Section: Plant Material Gamete Isolation and In Vitro Fertilisationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Novel ribosomal genes from maize are differentially expressed in the zygotic and somatic cell cycles

Dresselhaus¹,

Cordts²,

Heuer³

et al. 1999

Mol Gen Genet

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“…Novel breeding strategies that will allow improvement of genetic stocks need to be developed. In vitro fertilization (IVF) leads to the production of hybrids between plants that do not normally cross (Kranz and Lörz 1993;Faure et al 1994). Pollen transformation followed by IVF may enhance the efficiency of genetic transformation by taking advantage of the sexual reproduction route, resulting in development of stable, novel genotypes of economically superior trees.…”

Section: Introductionmentioning

confidence: 99%

In vitro pollen tube growth and penetration of female gametophyte in Douglas fir ( Pseudotsuga menziesii )

Fernando

Owens

Aderkas

et al. 1997

Sexual Plant Reproduction

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“…Since the first successful in vitro fertilization in angiosperms (Kranz et al 1991;Kranz and Lörz 1993) great advances have been made in this field (see Theunis et al 1991;Kranz and Dresselhaus 1996;Yang and Zhou 1998;Faure 1999). Most of the work has, however, been done on monocots, e.g.…”

Section: Introductionmentioning

confidence: 99%

In vitro double fertilization in Nicotiana tabacum (L.): fusion behavior and gamete interaction traced by video-enhanced microscopy

Sun¹,

Moscatelli

Hong-yuan

et al. 2000

Sexual Plant Reproduction

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In vitro double fertilization in tobacco was carried out with attention to fusion behavior and gamete interaction. Structural and cytological events indicating possible reaction to the fusion of sperm-egg and especially sperm-central cell were recorded by video-enhanced microscopy. Generative cells were fused with the egg cell or central cell as a control system to better understand gamete interaction. As early as adherence of the male cell, the female cell showed response by means of cytoplasm strand formation. After gamete fusion, cytoplasm activation in the egg cell was observed as long distance movement of organelles. In fertilized central cells, however, fusion did not result in notable cytological change within 30 min. Male nuclear movement recorded in the female cell illustrated two different patterns of movement which showed similarity to organelle movement. The dynamics of male and female nuclear fusion after in vitro fertilization was also recorded in the central cell. It revealed that the fusion process requires only a few seconds and is similar to that of gamete fusion in vitro. This may offer a new clue for understanding how female and male nuclei attract, adhere and finally fuse each other. Key words Nicotiana tabacum · In vitro fertilization · Gamete interaction · Cytology Material and methodsPlants of Nicotiana tabacum were grown in a greenhouse under controlled conditions in the

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In vitro Fertilization with Isolated, Single Gametes Results in Zygotic Embryogenesis and Fertile Maize Plants

Cited by 46 publications

References 29 publications

Novel ribosomal genes from maize are differentially expressed in the zygotic and somatic cell cycles

Novel ribosomal genes from maize are differentially expressed in the zygotic and somatic cell cycles

In vitro pollen tube growth and penetration of female gametophyte in Douglas fir ( Pseudotsuga menziesii )

In vitro double fertilization in Nicotiana tabacum (L.): fusion behavior and gamete interaction traced by video-enhanced microscopy

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