In vitro pollen tube growth and penetration of female gametophyte in Douglas fir ( Pseudotsuga menziesii )

Fernando, Danilo D.; Owens, John N.; Aderkas, Patrick von; Takaso, Tokushiro

doi:10.1007/s004970050089

Cited by 26 publications

(14 citation statements)

References 36 publications

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“…Therefore, the presence of corrosion cavities is not necessarily correlated with the occurrence of fertilization or the presence of developing proembryos, contrary to the reports of [17] and [29][30][31]. The presence of corrosion cavities in the absence of fertilization has also been documented in several studies involving in vitro culture of isolated female gametophytes [13][14][15].…”

Section: Sexual Reproduction In P Lambertianacontrasting

confidence: 52%

Sexual reproduction and crossing barriers in white pines: the case between Pinus lambertiana (sugar pine) and P. monticola (western white pine)

Fernando

Long

Sniezko

2005

Tree Genetics & Genomes

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The sexual reproductive process in Pinus lambertiana has not been completely described, and previous attempts to generate hybrids with Pinus monticola and other North American pines have not been successful. The nature of incompatibility barriers between P. lambertiana and P. monticola is unknown. This needs to be understood if strategies are to be developed to overcome the said barriers. In this paper, development on interspecific crosses is compared with that on intraspecific crosses on the same parent trees. Pollen grains of both species germinated on the nucellus of both species within a week after pollination. Seed cone receptivity in P. lambertiana came approximately 2 weeks after receptivity in P. monticola, and this delay was perpetuated throughout ovule development in the first year of the reproductive process. Development of the second-year seed cones proceeded more gradually in P. lambertiana. However, seed cones reached maturity only for P. monticola x P. lambertiana. In both crosses, the barriers to hybridization occurred during the second year of the reproductive process. With the P. lambertiana as the seed parent, it was manifested through the failure of the megaspores at the free-nuclear stage to resume development. When P. monticola was used as the seed parent, the male and female gametes failed to fuse. Our results clearly show that the barriers to hybridization in these species occur before or at fertilization. However, the exact mechanisms behind these are still unknown. Based on the results of this study, we present several strategies to bypass the developmental barriers and possibly produce hybrid progenies.

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Section: Sexual Reproduction In P Lambertianacontrasting

confidence: 52%

Sexual reproduction and crossing barriers in white pines: the case between Pinus lambertiana (sugar pine) and P. monticola (western white pine)

Fernando

Long

Sniezko

2005

Tree Genetics & Genomes

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“…In culture, pollen germination occurs after 1 or 2 days in Thuja plicata (Colangeli and Owens 1990a), Tsuga heterophylla (Colangeli and Owens 1990b), Picea glauca (Dawkins and Owens 1993), Larix occidentalis , Pseudotsuga menziesii (Webber and Painter 1996;Fernando et al 1997), Taxus brevifolia (Anderson and Owens 2000), Chamaecyparis nootkatensis (Anderson et al 2002), Abies amabilis (Chandler and Owens 2004), and Pinus contorta (Owens et al 2005). Although pollen germination in conifers is slow, they are generally easy to germinate in vitro and a very high germination percentage has been reported in various groups especially in Pinus.…”

Section: Pollen Tube Growth and Development In Vitromentioning

confidence: 97%

“…Ovule secretions have also been collected and used to induce pollen tubes in Pseudotsuga (Takaso et al 1996). Relatively higher rates of pollen tube formation, albeit at the 10-30% level, were obtained using optimized pollen germination media supplemented with flavonols (DumontBeBoux and von Aderkas 1996) or mineral salts (Fernando et al 1997).…”

Section: Pollen Tube Growth and Development In Vitromentioning

confidence: 99%

Growth and development of conifer pollen tubes

2005

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Conifer pollen tubes are an important but underused experimental system in plant biology. They represent a major evolutionary step in male gametophyte development as an intermediate form between the haustorial pollen tubes of cycads and Ginkgo and the structurally reduced and faster growing pollen tubes of flowering plants. Conifer pollen grains are available in large quantities, most can be stored for several years, and they grow very well in culture. The study of pollen tube growth and development furthers our understanding of conifer reproduction and contributes towards our ability to improve on their productivity. This review covers taxonomy and morphology to cell, developmental, and molecular biology. It explores recent advances in research on conifer pollen and pollen tubes in vivo, focusing on pollen wall structure, male gametophyte development within the pollen wall, pollination mechanisms, pollen tube growth and development, and programmed cell death. It also explores recent research in vitro, including the cellular mechanisms underlying pollen tube elongation, in vitro fertilization, genetic transformation and gene expression, and pine pollen tube proteomics. With the ongoing sequencing of the Pinus taeda genome in several labs, we expect the use of conifer pollen tubes as an experimental system to increase in the next decade.

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“…Thirty to 60 unshed staminate strobili were collected per tree, surface sterilized and allowed to shed under sterile conditions following the procedure described by Fernando et al (1997). Pollen was plated on 8 % sucrose Brewbaker and Kwack (1963) medium, incubated at 248C in continuous darkness.…”

Section: Methodsmentioning

confidence: 99%

Microsporogenesis, Pollination, Pollen Germination and Male Gametophyte Development in Taxus brevifolia

Anderson

Owens

2000

Annals of Botany

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In vitro pollen tube growth and penetration of female gametophyte in Douglas fir ( Pseudotsuga menziesii )

Cited by 26 publications

References 36 publications

Sexual reproduction and crossing barriers in white pines: the case between Pinus lambertiana (sugar pine) and P. monticola (western white pine)

Sexual reproduction and crossing barriers in white pines: the case between Pinus lambertiana (sugar pine) and P. monticola (western white pine)

Growth and development of conifer pollen tubes

Microsporogenesis, Pollination, Pollen Germination and Male Gametophyte Development in Taxus brevifolia

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