In vitro fertilizing capacity of human spermatozoa with the use of zona-free hamster ova: interassay variation and prognostic value

Cohen, Jacques; Weber, R. F. A.; Vijver, J. C. M. Van Der; Zeilmaker, G. H.

doi:10.1016/s0015-0282(16)46167-4

Cited by 58 publications

(21 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the donor group the mean penetration rate of 53% coincided with the results of similar groups in different studies where the mean penetration rates in donor and fertile men were 56.3% [14], 54% [6], 55.9% [16], and 59.7% [15]. The mean penetration obtained in this study is low compared with some studies: 81% [19], 69% [ l o ] , and 66% [7].…”

Section: Discussionsupporting

confidence: 86%

“…It is considered superior t o the routine spermiogram in discriminating the fertile from the infertile men [6,7,13,141 and offers a biological assessment of the human sperm fertilizing ability. The test is performed by inseminating a number of the zona-free hamster eggs with precapacitated human sperm for a few hours; according to the percentage of the ova penetrated, the semen sample is rated as fertile or nonfertile.…”

Section: Yanagimachi Et Al [ 181mentioning

confidence: 99%

See 1 more Smart Citation

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Hamdi

Ibrahim

Girgis

et al. 1985

Archives of Andrology

View full text Add to dashboard Cite

The zona-free hamster egg penetration test was performed on semen samples from 21 fertile donors, 57 infertile patients with normal semen parameters, and 63 infertile patients with subnormal spermiogram, as well as on 19 frozen semen samples.In the donor group 100% of the samples gave a positive range of penetration (PRP) of 20-96% with a mean penetration rate (MPR) of 53%, while 84% of the frozen samples showed a PRP of 18-44% and an MPR of 31%.Although 74% of the samples from infertile patients with normal spermiograms gave a PRP of 11-9270 and an MPR of 34%, only 46% of the semen samples from the infertile patients with subnormal spermiogram showed a PRP of 21-100% but an MRP of 43%.

show abstract

Section: Discussionsupporting

confidence: 86%

Section: Yanagimachi Et Al [ 181mentioning

confidence: 99%

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Hamdi

Ibrahim

Girgis

et al. 1985

Archives of Andrology

View full text Add to dashboard Cite

show abstract

“…Although an interspecific in-vitro fertilization procedure was used in this analysis, the details of sperm-egg interaction in this system appear to reflect the events in homologous fertilization, particularly with respect to the fusion process (Koehler, De Curtis, Stenchever & Smith, 1982). Furthermore, the outcome of this procedure has been found to correlate with human fertility in retrospective (Cohen, Weber, van der Vijver & Zeilmaker, 1982;Aitken et al, 1982) and prognostic (Cohen et al, 1982;Aitken, 1983) (Amann, 1981) while the entire transit time from spermiation to ejaculation has been estimated at 12 days (Rowley, Teshima & Heller, 1970). Allowing 5-5 days for epididymal passage and storage (Amann, 1981), the average amount of time spent by human spermatozoa in extra-epididymal sites should be about 7-5 days ; a period that corresponds to that in which spermatozoa with fertilizing ability were obtained in the present study.…”

Section: Resultsmentioning

confidence: 99%

The functional competence of human spermatozoa recovered after vasectomy

Richardson¹,

Aitken²,

Loudon³

1984

Reproduction

View full text Add to dashboard Cite

Summary. Progresssively motile spermatozoa were present in 42/47 (89%) of post \ x=r eq-\ vasectomy specimens, and in 16 of 24 samples (66%) demonstrable fertilizing potential could be detected in the zona-free hamster egg assay. The mean \m=+-\ s.e.m. post \ x=req-\ vasectomy penetration rate of 20\m=.\6 \m=+-\6\m=.\0%was, however, significantly lower (P < 0\m=.\001) than the pre-vasectomy value of 49\m=.\7 \m=+-\6\m=.\4%. After vasectomy the concentration of motile spermatozoa in the ejaculates declined in a manner that was significantly correlated with the post-operative time interval. The spermatozoa also lost their potential for fertilization, although functionally competent spermatozoa were still being recovered from ejaculates produced 8 days after vasectomy.These results emphasize the ability of human spermatozoa to retain some residual fertilizing potential when stored at core body temperature in sites distal to the epididymis, and suggest a role for functional tests in the management of the vasectomized male.

show abstract

“…Test reliability has been studied by several authors (Binor et al, 1980 ;Cohen et al, 1982 ;Hall, 1981 ;Junca et al, 1982 ;Karp et al, 1981 ;Tyler et al, 1981 ;Zausner-Guelman et al, 1981 …”

Section: Discussionmentioning

confidence: 99%

Zona-free hamster ovum penetration by human spermatozoa : influence of various sperm factors

Courtot¹,

Jouannet²

1985

Reprod. Nutr. Dévelop.

View full text Add to dashboard Cite

Summary. The present paper evaluates the influence of various technical factors when preparing human spermatozoa to be tested for their ability to penetrate zona-free hamster ova. Higher in vitro fertilization rates were obtained after sperm selection by « swim-up » migration, 4-h incubation, sperm dilution to a concentration of 2.5 x 10 6 spermatozoa/ml, and observation of the oocytes 8 h after insemination. The use of control samples was necessary because of wide intraindividual and interindividual variations in the results.Unless the result has been confirmed several times, it is impossible to conclude, from a negative test, that a human spermatozoon cannot penetrate a hamster oocyte.Introduction.

show abstract

In vitro fertilizing capacity of human spermatozoa with the use of zona-free hamster ova: interassay variation and prognostic value

Cited by 58 publications

References 15 publications

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

Zona-Free Hamster Egg-Sperm Penetration Assay: I. Testing of Human Semen Fertilizability

The functional competence of human spermatozoa recovered after vasectomy

Zona-free hamster ovum penetration by human spermatozoa : influence of various sperm factors

Contact Info

Product

Resources

About