In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

Jingjit, Suttinee; Kanchanapoom, Kamnoon

doi:10.15835/nbha3914994

Cited by 15 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Inclusion of higher concentration of auxins suppressed the regeneration pathway as it induced basal callusing, which is not desirable for direct shoot regeneration. Our results substantiate with earlier findings in Adenium obesum (Kanchanapoom et al 2010), Lippia nodiflora (Priya and Ravindhran, 2011) and Vitex negundo (Usha et al 2007;Ahmad et al 2013). Thus, it can be inferred from the above results that the interactive effect of BA and NAA could ensure better in vitro regeneration and their synergism in proper concentration was extremely favourable for multiplication.…”

Section: Multiplication and Proliferation Of Shootssupporting

confidence: 93%

See 1 more Smart Citation

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Ahmed

Anis

2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

A rapid and efficient plant propagation system through shoot tip explants was established in Vitex trifolia L., a medicinally important plant belonging to the family Verbenaceae. Multiple shoots were induced directly on Murashige and Skoog (MS) medium consisting of different cytokinins, 6-benzyladenine (BA), kinetin (Kin) and 2-isopentenyl adenine (2-iP), BA at an optimal concentration of 5.0 μM was most effective in inducing multiple shoots where 90 % explants responded with an average shoot number (4.4 ±0.1) and shoot length (2.0±0.1 cm) after 6 weeks of culture. Inclusion of NAA in the culture medium along with the optimum concentration of BA promoted a higher rate of shoot multiplication and length of the shoot, where 19.2±0.3 well-grown healthy shoots with an average shoot length of 4.4±0.1 cm were obtained on completion of 12 weeks culture period. Ex vitro rooting was achieved best directly in soilrite when basal portion of the shoots were treated with 500 μM indole-3-butyric acid for 15 min which was the most effective in inducing roots, as 95 % of the microshoots produced roots. Plantlets went through a hardening phase in a controlled plant growth chamber, prior to ex-vitro transfer. Micropropagated plants grew well, attained maturity and flowered with 92 % survival rate. The results of this study provide the first report on in vitro plant regeneration of Vitex trifolia L. using shoot tip explants.

show abstract

Section: Multiplication and Proliferation Of Shootssupporting

confidence: 93%

“…1c, d). Such stimulating effects of combinations of these two hormones are well supported by earlier studies on Adenium obesum (Kanchanapoom et al 2010) and Vitex negundo (Usha et al 2007, Ahmad et al 2013). This positive effect of BA+NAA on differential morphogenetic response may be due to apical dominance.…”

Section: Multiplication and Proliferation Of Shootssupporting

confidence: 59%

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Ahmed

Anis

2014

Physiol Mol Biol Plants

View full text Add to dashboard Cite

show abstract

“…In vitro production of flowers is especially important for ornamental plants such as orchids (Hee et al 2007), roses (Kanchanapoom et al 2010b), jasminum (Jumin and Ahmad 1999), bamboo (John and Nadgauda 1999;Lin et al 2003;Ramanayake et al 2001), among others (Bodhipadma et al 2011;Hu et al 2010;Kanchanapoom et al 2010a;Taha et al 2010). In these species, early in vitro flowering of micropropagated plantlets shortens the breeding cycle to meet market demands.…”

Section: Introductionmentioning

confidence: 99%

In vitro flowering and production of viable pollen of cucumber

Kiełkowska

Havey

2011

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Flowers were produced on sterile cucumber (Cucumis sativus L.) plants grown in vitro from seed and micropropagated shoots from stem fragments. The highest numbers of flowers on plants from both sources were produced on Murashige and Skoog (MS) medium without plant growth regulators (PGR), as well as with 6 lM of kinetin (Kin). Plants cultured on MS medium supplemented with 8.9 lM benzyladenine (BA) and 1.1 lM 1-naphthaleneacetic acid (NAA) did not flower. In vitro grown plants produced fewer, smaller flowers compared with greenhouse-grown plants. Male and female flowers developed on plants grown in vitro from seed and were morphologically similar to flowers on greenhouse grown plants. Micropropagated shoots produced male flowers with altered morphology. The highest viability (72.9 ± 4.2%) and germination (69.5 ± 4.1%) of pollen were observed for plants grown from seed on MS medium supplemented with 6 lM Kin. Cytological observations of meiosis in anthers of male flowers from in vitro grown plants revealed abnormalities, such as monads, dyads, triads, polyads, microcytes and degeneration of tetrads, causing reduced viability and germination of pollen. The fewest meiotic irregularities in pollen mother cells were observed in plants grown on MS medium that was PGRfree (12.1 ± 0.9%) or with 6 lM Kin (20.9 ± 1.7%).

show abstract

“…The highest number of stems per plant node (n=3) belonged to the medium containing 0.5 milligrams per liter of BA + 0.5 mg/l naphthalene acetic acid, plus0.2 mg/l BA + control NAA acid acetic, but the difference with other treatments was notstatistically significant at 5%. Kanchanapoom et al (2011) stated that BA had increased (the number of) stems in Gypsophila paniculata. 0.05 ppm NAA +0.05 ppm BA and 0.2 ppm NAA +0.5 ppm BA were the only treatments that possessed embryogenic callus.…”

Section: Discussionmentioning

confidence: 99%

Investigating In vitro micro propagation of (Saponaria officiale L) by nodal culture with sucrose supplement

2016

Sci. Agric

View full text Add to dashboard Cite

Medicinal soap, scientifically known as (saponaria officinalis L), is a perennial plant belonging to the family of carnation and a valuable medicinal and industrial plant. The origin of this plant is the Mediterranean region. The medicinal properties of plant soap include blood filtering, elimination of rheumatism, gout, urinary tract and skin diseases, cure cancer, eczema and tuberculosis. The purpose of this study was to investigate medicinal soap plant micro-propagation using explantinternodes in a medium containing 50 grams of sucrosein the presence of hormone levels of BA (0 -0.05 -0.2-0.5) and naphthalene acetic acid (0 -0.05 -0.2-0.5). Different factors appraisedincluded fresh weight, root and stem length, leaf area, length and width of callus, number of stems and roots, embryogenetic plants, aerial root, root hair, green leaves, red leaves, lost leaves, number of nodesand stem produced from each node and color of callus. The mean comparison showed that the freehormone medium conducive to the increment of root length, the medium containing 0.5 mg/l BA and 0.5 mg/lnaphthalene acetic acid (NAA) increased the number of red leaves, length of plant, as well as the number of stems growing from each node,the medium without BA, containing 0.5 mg/l NAA increased the number of green leaves, the number of nodes, length and widthof leaves, medium containing 0.5 mg/l BA and NAA 0.05 mg per liter gave rise to dead leaves, medium containing 0.5 milligrams per liter BA and without naphthalene acetic acid affected the number of roots, plant fresh weight and length of callus, the medium0.05mg/l BA and 0.05mg/l naphthalene acetic acid increased the length of leaves and the number of embryo genetic plants. The medium containing Mg / liter BA with 0.2 mg/l naphthalene acetic acid had an increasing effect on hair roots and the medium containing 0.05 mg/l BA and 0.5 mg per liter NAA were effective in increasing the width of the callus.

show abstract

In vitro Flowering of Shoots Regenerated from Cultured Nodal Explants

Cited by 15 publications

References 12 publications

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

Rapid in vitro propagation system through shoot tip cultures of Vitex trifolia L.–an important multipurpose plant of the Pacific traditional Medicine

In vitro flowering and production of viable pollen of cucumber

Investigating In vitro micro propagation of (Saponaria officiale L) by nodal culture with sucrose supplement

Contact Info

Product

Resources

About