In vitro function of random donor platelets stored for 7 days in composol platelet additive solution

Abstract

Background and Aim:Platelets are routinely isolated from whole blood and stored in plasma for 5 days. The present study was done to assess the in vitro function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C.Materials and Methods:The study sample included 30 blood donors of both sex in State Blood Bank, CSM Medical University, Lucknow. Random donor platelets were prepared by platelet rich plasma method. Whole blood (350 ml) was collected in anticoagulant Citrate Phosphate Dextrose Adenine triple blood bags. Random donor platelets were stored for 7 days at 22°C in platelet incubators and agitators, with and without additive solution.Results:Platelet swirling was present in all the units at 22°C on day 7, with no evidence of bacterial contamination. Comparison of the mean values of platelet count, platelet factor 3, lactate dehydrogenase, pH, glucose and platelet aggregation showed no significant difference in additive solution, whereas platelet factor 3, glucose and platelet aggregation showed significant difference (P < 0.001) on day 7 without additive solution at 22°C.Conclusion:Our study infers that platelet viability and aggregation were best maintained within normal levels on day 7of storage in platelet additive solution at 22°C. Thus, we may conclude that in vitro storage of random donor platelets with an extended shelf life of 7 days using platelet additive solution may be advocated to improve the inventory of platelets.

Assessment of quality of platelets preserved in plasma and platelet additive solution: A Malaysian experience

“…[ 1 ] and Gupta et al . [ 7 ] observed significantly lower values in pH in platelets preserved in PAS as compared to that preserved in plasma, though the magnitude of difference was well within the acceptable limit. In the present study, we found a gradual decline in pH for both the platelet suspending medium, i.e., plasma and PAS.…”

“…Van der Meer[ 12 ] obtained a higher yield of platelets suspended in plasma as compared to that in PAS and it was attributed to the lower viscosity of the PAS under given centrifugation conditions. Gupta et al .,[ 7 ] on the other hand, found no significant difference in total platelet count irrespective of the PC suspended in plasma or in Composol. In the present study, the median value of total platelet count from both suspending medium is within acceptable limit of >60 × 10 9 /unit, though the higher values of total platelet count in PAS was not significantly different from that in plasma ( P > 0.005).…”

“…Result from sterility testing showed no growth for both group of PC suspended in plasma and PAS and this observation was line with other studies. [ 7 ] Bacterial contamination has been the main concern in extending PC shelf life. Based on this study, it has emerged that the sterility of the PCs can be maintained through the extended shelf life up to 8 days.…”

“…The weight of the PC suspension was kept to 60-70 g. While 19 PCs were kept in native plasma, the other 19 PCs were suspended in PAS (Composol PS, Fresenius Kabi) fortified with native plasma in an approximate ratio between 70:30 and 65:35. [ 7 ] For this, an amount of plasma was retained in the bag with the platelet and the amount of PAS was then manually added so as to achieve the desired ratio. The ratio was maneuvered approximately between 70:30 and 65:35 but, as it was done manually, it was not possible to control the exact amount of additive to plasma.…”