In Vitro Functional Reactivities of Cutaneous Mast Cells From Patients With Mastocytosis

Tharp, Michael D.; Chaker, Basem; Glass, Marcia J.; Burton, Rebecca A.B.; Seelig, Leonard L.

doi:10.1111/1523-1747.ep12471299

Cited by 15 publications

(6 citation statements)

References 27 publications

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“…The very small proliferative compartment in cutaneous mastocytosis is in accord with the favorable prognosis 27 . It is also consistent with the observation that dividing mast cells are seen by electron microscopy in the lesional skin of cutaneous mastocytosis 28–30 …”

Section: Discussionsupporting

confidence: 86%

Alteration of mast cell proliferation/apoptosis and expression of stem cell factor in the regression of mastocytoma – report of a case and a serial immunohistochemical study

et al. 2002

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Section: Discussionsupporting

confidence: 86%

Alteration of mast cell proliferation/apoptosis and expression of stem cell factor in the regression of mastocytoma – report of a case and a serial immunohistochemical study

et al. 2002

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“…Inflammatory cell infiltrates in the lesion may also be induced by the MC degranulation (17,18). In this study, the presence or absence of symptoms, Darier's sign, and inflammatory cell infiltrates failed to cor- (19). However, IgE deposition on MC in UP lesions has not been detected (20).…”

Section: Discussioncontrasting

confidence: 50%

A Clinical and Histological Study of Urticaria Pigmentosa: Relationships between Mast Cell Proliferation and the Clinical and Histological Manifestations

Akiyama

1990

The Journal of Dermatology

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In 21 cases of urticaria pigmentosa (UP), clinical and histological observations and evaluation of mast cell (MC) volume density in the lesions using a morphometric point counting method were performed. The mutual correlations between clinical and histological findings were statistically assessed by a method of multiple regression analysis. Clinical items employed in the analyses were as follows: sex, the age of onset, the age of biopsy, the biopsy, the duration of lesions, the type of skin lesions, sites involved, the presence or absence of Darier's sign of Darier's sign and symptoms, and serum histamine level. Histological items included the localization and infiltration pattern of MC, the level of basal melanosis, the presence or absence of inflammatory cell infiltration, and the MC volume density in the lesions. Statistical significance of the partial regression coefficients was obtained for 6 pairs of the criteria (p = 0.05), including the age of onset and the age of biopsy, the age of onset and the level of basal melanosis, the duration of lesions and the level of basal melanosis, and the type of skin lesions and the level of basal melanosis. No significant correlations were observed between the MC volume density in the lesions and any of the other items. These results suggest that the basal melanosis in a UP lesion may not be a direct reaction to the transitory massive infiltration of MC, but rather be due to a relatively long-term effect of MC infiltration. Furthermore, the MC volume density in the lesion is not likely to be an important factor in determining the clinical manifestations of a UP lesion.

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“…IgE deposition was not detected on mast cells in UP lesion. Tharp et al [27] reported that mast cells from lesions of mastocytosis retained their functional reac tivities to secretory stimuli via IgE. If this is true in our case, the absence of IgE deposition on mast cells may not reflect the absence of receptors for IgE.…”

Section: Discussionmentioning

confidence: 55%

Peroxidase Activity in Mast Cell Granules in Urticaria pigmentosa

Akiyama

Watanabe²,

Nishikawa³

1989

Dermatology

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The morphology of mast cells in the skin lesion from a case of urticaria pigmentosa was observed using light and electron microscopy. Furthermore, the IgE deposition on mast cells in the lesion was studied with immunoelectron microscopy. Diaminobenzidine reaction and 4-chloro-naphthol reaction were used to detect endogenous peroxidase activity in mast cells in the lesion. Ultracytochemical techniques were applied to clarify the localization of peroxidase activity in the mast cells. Morphologically, neither blast-like cells nor intermediate cells between basophils and mast cells were found in the lesion. No mast cells in the lesion did exhibit IgE deposition on their plasma membrane. By light microscopy, granular stains of diaminobenzidine reaction and 4-chloro-naphthol reaction were observed in the cytoplasm of mast cells in the lesion. By electron microscopy, a finely stippled peroxidase staining was observed in some granules of the mast cells. Perinuclear cisterna and endoplasmic reticulum appeared devoid of enzymatic activity. These findings suggest that the infiltrating mast cells in this case were immature.

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In Vitro Functional Reactivities of Cutaneous Mast Cells From Patients With Mastocytosis

Cited by 15 publications

References 27 publications

Alteration of mast cell proliferation/apoptosis and expression of stem cell factor in the regression of mastocytoma – report of a case and a serial immunohistochemical study

Alteration of mast cell proliferation/apoptosis and expression of stem cell factor in the regression of mastocytoma – report of a case and a serial immunohistochemical study

A Clinical and Histological Study of Urticaria Pigmentosa: Relationships between Mast Cell Proliferation and the Clinical and Histological Manifestations

Peroxidase Activity in Mast Cell Granules in Urticaria pigmentosa

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