In Vitro Gene Delivery by pDNA/Chitosan Complexes Coated with Anionic PEG Derivatives that Have a Sugar Side Chain

Abstract

We developed pDNA/chitosan complexes coated with sugar-modified PEG-A/Cs, which are poly(ethylene glycol) derivatives with a side chain of carboxylic acid and sugar. The cationic pDNA/chitosan complexes were coated with anionic sugar-modified PEG-Cs, and anionic ternary complexes were formed. Coating the pDNA/chitosan complexes with maltose- or lactose-modified PEG-A/C (Mal–PEG-A/C or Lac–PEG-A/C, respectively) greatly promoted their stability in water and transfection efficiency in vitro.

In vitro and in vivo gene delivery using chitosan/hyaluronic acid nanoparticles: Influences of molecular mass of hyaluronic acid and lyophilization on transfection efficiency

“…However, they also elicited serum‐induced aggregation and an absence of significant gene expression in tumor‐implanted mice . To overcome these issues, pDNA/chitosan complexes were coated with CS and anionic PEG derivatives in our previous study . Notably, the pDNA/chitosan/CS complexes reduced serum‐induced aggregation and induced high transgene expression in vitro and in vivo.…”

“…10 To improve the stability and transgene expression of pDNA/chitosan complexes, Hashimoto et al 11,12 performed chemical modifications of chitosan with sugars. Furthermore, cationic pDNA complexes were coated with polyanions such as polyethylene glycol (PEG) derivatives, 13 hyaluronic acid (HA), [14][15][16][17] chondroitin sulfate (CS) 9,10,[18][19][20] and poly(γ-glutamic acid). 21 In our previous study, physicochemical properties and the transfection abilities of pDNA/chitosan complexes coated with CS were investigated and it was observed that CS demonstrates excellent gene carrier properties.…”

Physicochemical Properties of pDNA/Chitosan Complexes as Gene Delivery Systems

“…To address these limitations, ternary complexes prepared by coating the cationic DNA/polycation complex with anionic polymer have been developed [49,50].…”

“…Hashimoto et al developed the pDNA/chitosan complexes coated with lactose-modified PEG-C derivatives, namely pDNA/chitosan/Lac-PEG-C ternary complexes (Fig. 7) [50]. Zeta potential of the pDNA/chitosan/Lac-PEG-C ternary complexes became negative by coating the cationic pDNA/chitosan complexes with anionic Lac-PEG-C.…”

Preparation and characterization of low-molecular-weight heparin/protamine nanoparticles (LMW-H/P NPs) as FGF-2 carrier

“…[1][2][3][4] An interest in such electrostatic interactions lies in their similarity to biological systems; 7 such interactions between proteins and nucleic acids very likely play a role in the transcription process. 5 DNA/chitosan PECs, 8 chiosan/chondroitin sulfate PECs and chitosan/hyaluronate PECs 9 have been reported as gene and 10 Heparinoids are known to specifically interact with variety of functional proteins, including growth factors, cytokines, extracellular matrix components and adhesion molecules. [11][12][13] Thus, heparin may be useful as a therapeutic agent in various pathological conditions involving functional proteins.…”