In Vitro Genotoxicity of Settat Town Landfill Leachate, Morocco

Amahdar, Loubna; Anouar, Abdellah; Ababou, Bouchra; Verschaeve, Luc; Hilali, Abderraouf

doi:10.2478/10004-1254-60-2009-1925

Cited by 14 publications

(2 citation statements)

References 24 publications

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“…The genetic effects of solid waste leachate have been examined in bacterial, plant, animal, and human cells (Amahdar et al, 2009;Bakare et al, 2005;Deguchi et al, 2007;Sang and Li, 2005;Singh et al, 2007). It has been suggested that DNA damage induced by leachates might be due to the presence and interactions of heavy metals with DNA.…”

Section: Discussionmentioning

confidence: 99%

DNA and oxidative damage induced in somatic organs and tissues of mouse by municipal sludge leachate

et al. 2011

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Pollution by waste landfill leachate has prompted a number of studies on the toxic and potential health effects. This study assessed the genotoxicity of a municipal sludge leachate (MSL) in the somatic tissues (blood and bone marrow) and organs (liver, kidney, and spleen) of mice using the alkaline Comet assay. The possible cause of DNA damage via the study of antioxidant system (lipid peroxidation [LPO]; catalase [CAT]; reduced glutathione [GSH]; and superoxide dismutase [SOD]) responses in mouse liver was also investigated. Different concentrations (2.5%, 5%, 10%, and 15%) of the leachate were administered intraperitoneally for 5 consecutive days to male Swiss albino mice (4 mice/group). A significant (p < 0.05) increase in DNA damage in organs and tissues of treated mice compared to the negative control was observed as evident from the Comet assay parameters: olive tail moment (OTM, arbitrary units) and tail DNA (%). Bone marrow showed maximum DNA damage followed by liver > spleen > kidney > blood as evident by the OTM. A significant increase (p < 0.05) in the level of antioxidant enzymes (CAT and SOD) and LPO with a concurrent decrease in GSH in the liver of treated mice was also observed. Our finding demonstrates that the MSL induces DNA damage in the somatic tissues and organs of mouse as well as induces oxidative stress in the liver. These tissues and organs may be the potential targets in animal and human populations exposed to MSL. This is of relevance to public health; as such exposure could lead to adverse health effects via systemic genotoxicity.

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Section: Discussionmentioning

confidence: 99%

DNA and oxidative damage induced in somatic organs and tissues of mouse by municipal sludge leachate

et al. 2011

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“…Some of the heavy metals found in concentrations above limits, are the likely culprits such as lead, chrome, aluminium, and cadmium, as they may in certain forms be mutagenic. In general, metal genotoxicity is caused by indirect mechanisms, of which three are predominant (25): a) interference with cellular redox regulation and induction of oxidative stress, which may cause oxidative DNA damage [also see (26)]; b) inhibition of major DNA repair systems, resulting in genomic instability and accumulation of critical mutations [also see (26)]; and that adding volumes of up to 800 µL did not infl uence cell cultures and did not induce a toxic response, e.g., as a result of changed osmolarity (19).…”

Section: Figure 2 Micronucleus Frequency In Human Peripheral Blood Lymentioning

confidence: 99%

In Vitro Genotoxicity of Wastewaters from the Town of Settat, Morocco

Asslouj

Amahdar

Glouib

et al. 2009

Archives of Industrial Hygiene and Toxicology

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In recent years, the town of Settat has seen a considerable industrial growth, which has resulted in increased environmental pollution. This includes pollution by household and industrial wastewaters, which are released into the Boumoussa River without any preliminary treatment. The river valley crosses the community of Mzamza 8 km to the north of the town. Years of drought forced members of the community to use this polluted ground water for irrigation and put themselves and the environment at risk. The aim of this study was to determine the physicochemical and metal profi le of Settat wastewaters and to assess their impact on the water table. The second objective was to investigate the genotoxic potential of wastewater on human peripheral blood lymphocytes in vitro, using the micronucleus test and cellular proliferation index. This study demonstrated signifi cant pollution of Boumoussa valley groundwater and of the local wells. Sampled water induced a clear increase in the frequency of micronucleated cells and a lower cell proliferation in human peripheral blood lymphocytes in vitro.

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