In vitro genotoxicity tests for polyhydroxybutyrate – A synthetic biomaterial

Ali, Abdulaziz Qaid; Kannan, Thirumulu Ponnuraj; Ahmad, Azlina; Samsudin, Abdul Rani

doi:10.1016/j.tiv.2007.08.001

Cited by 23 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PHB was discovered by Lemoigne in the bacterium Bacillus megaterium in 1926 . It was the first polymer of the PHAs class to be discovered and it is also the most widely studied and the best characterized PHA.…”

Section: Introductionmentioning

confidence: 99%

Influence of electrospinning parameters on poly(hydroxybutyrate) electrospun membranes fiber size and distribution

Correia

Ribeiro

Ferreira

et al. 2013

Polymer Engineering & Sci

View full text Add to dashboard Cite

Poly(hydroxybutyrate) (PHB) obtained from sugar cane was dissolved in a blend of chloroform and dimethylformamide (DMF) and electrospun at 40 ºC. By adding DMF to the solution, the electrospinning process for the PHB polymer becomes more stable, allowing complete polymer crystallization during the jet travelling between the tip and the grounded collector.The influence of processing parameters on fiber size and distribution was systematically studied. It was observed that an increase of tip inner diameter promotes a decrease of the fiber average size and a broader distribution. On the other hand, an increase of the Polymer Engineering & Science DOI: 10.1002/pen.23704 2 electric field and flow rate produces an increase of fiber diameter until a maximum of ~2.0 m, but for electric fields higher than 1.5 kV.cm -1 , a decrease of the fiber diameter was observed. Polymer crystalline phase seems to be independent of the processing conditions and a crystallinity degree of 53 % was found. Moreover, thermal degradation of the as-spun membrane occurs in single step degradation with activation energy of 91 kJ/mol. Furthermore, MC-3T3-E1 cell adhesion was not inhibited by the fiber mats preparation, indicating their potential use for biomedical applications. Keywords

show abstract

Section: Introductionmentioning

confidence: 99%

Influence of electrospinning parameters on poly(hydroxybutyrate) electrospun membranes fiber size and distribution

Correia

Ribeiro

Ferreira

et al. 2013

Polymer Engineering & Sci

View full text Add to dashboard Cite

show abstract

“…It is also characterized by high brittleness, poor processability and poor thermal stability [8]. Due to its natural origin, PHB has potential for biomedical applications, including drug delivery systems [9]. It has been evaluated for controlled drug release systems, surgical structures, wound dressings, orthopedic devices, tissue engineering and skin substitute materials [10].…”

mentioning

confidence: 99%

PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications

et al. 2014

View full text Add to dashboard Cite

Fiber meshes of poly(hydroxybutyrate) (PHB) and poly(hydroxybutyrate)/ poly(ethylene oxide) (PHB/PEO) with different concentrations of chlorhexidine (CHX) were prepared by electrospinning, for assessment as a polymer based drug delivery system. The electrospun fibers were characterized at morphological, molecular and mechanical levels. The bactericidal potential of PHB and PHB/PEO electrospun fibers with and without CHX was investigated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by disk diffusion susceptibility tests. Electrospun fibers containing CHX exhibited bactericidal activity. PHB/PEO-1%CHX displayed higher CHX release levels and equivalent antibacterial activity when compared to PHB/PEO with 5 and 10 wt% CHX. Bactericidal performance of samples with 1 wt% http://www.sciencedirect.com/science/article/pii/S0142941813002493 2 CHX was assessed by Colony Forming Units (CFU), where a reduction of 100 % and 99.69 % against E. coli and S. aureus were achieved, respectively.

show abstract

“…Their results confirmed no tumor induction when proliferating rat osteoblasts were grown on PHB films. Another study on fibroblasts has proven that PHB is not genotoxic and did not alter the expression of the proto-oncogenes and anti-apoptotic genes analyzed in the study (51). PHB and biphasic calcium phosphate/chitosan and showed increased proliferation of rat primary osteoblasts on membranes.…”

Section: In Vitro Biocompatibility Of Phb On Bone Cellsmentioning

confidence: 74%

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Dariš

Knez

2019

Acta Pharmaceutica

View full text Add to dashboard Cite

Poly(3-hydroxybutyrate) is a natural polymer, produced by different bacteria, with good biocompatibility and biode gradability. Cardiovascular patches, scaffolds in tissue engineering and drug carriers are some of the possible biomedical applications of poly(3-hydroxybutyrate). In the past decade, many researchers examined the different physicochemical modifications of poly(3-hydroxybutyrate) in order to improve its properties for use in the field of bone tissue engineering. Poly(3-hydroxybutyrate) composites with hydroxy apatite and bioglass are intensively tested with animal and human osteoblasts in vitro to provide information about their bio com patibility, biodegradability and osteoinductivity. Good bone regeneration was proven when poly(3-hydroxybutyrate) patches were implanted in vivo in bone tissue of cats, mini pigs and rats. This review summarizes the recent reports of in vitro and in vivo studies of pure poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate) composites with the emphasis on their bioactivity and biocompatibility with bone cells.

show abstract

In vitro genotoxicity tests for polyhydroxybutyrate – A synthetic biomaterial

Cited by 23 publications

References 49 publications

Influence of electrospinning parameters on poly(hydroxybutyrate) electrospun membranes fiber size and distribution

Influence of electrospinning parameters on poly(hydroxybutyrate) electrospun membranes fiber size and distribution

PHB-PEO electrospun fiber membranes containing chlorhexidine for drug delivery applications

Poly(3-hydroxybutyrate): Promising biomaterial for bone tissue engineering

Contact Info

Product

Resources

About