In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey

Bektaş, Ersan; Cüce, Mustafa; Sökmen, Atalay

doi:10.3906/bot-1205-28

Cited by 22 publications

(9 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The highest protocorm formation (83.14%) was observed when OM+ basal medium was supplemented with 2.0 mg/L ZEA. This finding is similar to our previous report (Bektaş et al, 2013). OM+ free of PGRs was used as control.…”

Section: Resultssupporting

confidence: 93%

“…Plant tissue culture media play an important role in ensuring these environmental conditions. Bektaş et al (2013) and Bektaş and Sökmen (2016) reported that the Orchimax including activated charcoal culture medium (OM+) was the most effective basal culture media on the germination of Orchis coriophora and Serapais vomeracea mature seeds, similar to our results. Murthy, 1977;Malmgren, 1992;Vesjadova, 2006).…”

Section: Resultssupporting

confidence: 90%

“…The plantlets were transferred to the sterile peat medium in the first stage and then successfully adhered to the soil conditions (Figure 4). This gradual adaptation process, which was previously carried out with Serapias vomeracea, may give fruitful results and could be applied to other orchid species (Bektaş et al, 2013).…”

Section: Resultsmentioning

confidence: 94%

“…As a result, cytokinins were proven to be more effective in the germination of O. sancta seeds. Cytokinins have been reported as PGRs with the most favorable effect on the germination of terrestrial orchid seeds (Bektaş et al, 2013). Cytokinins have also been reported to promote seed germination by reducing or removing the inhibitory effect of abscisic acid on germination (Crafts & Miller, 1974).…”

Section: Resultsmentioning

confidence: 99%

“…The most ideal, fast and controlled reproduction procedure that can be applied for the production of these species is "propagating plants or products using tissue culture techniques". Nevertheless, such approaches are comparably new and therefore only handful information is available in the literature (Sgarbi et al, 2009;Bektaş et al, 2013;Bektaş & Sökmen, 2016). Also, it should be born in mind that in vitro production methods applied may vary from one species to another.…”

mentioning

confidence: 99%

See 4 more Smart Citations

In vitro asymbiotic germination, seedling formation and tuberization of Orchis sancta

Bektaş

Sökmen

2022

Turkish Journal of Biodiversity

Self Cite

View full text Add to dashboard Cite

Bitki doku kültürü teknikleri, son zamanlarda, nadir ve nesli tükenmekte olan türlerin çoğaltılması ve ekonomik açıdan önemli doğal ürünlerinin üretilmesi için ilgi çekici araçlar haline gelmiştir. Bu çalışmada, Türkiye'de doğal olarak yetişen yerli bir sahleb orkidesi türü olan Orchis sancta L.’nın fide ve yumrularının in vitro koşullarda etkin, hızlı ve güvenilir bir üretim protokolü oluşturulmuştur. Buna istinaden, olgun tohumların asimbiyotik çimlenmesi, fide oluşumu, yumru oluşumu ve yumru köklerin glukomannan içerikleri üzerine bazal ortam ve bitki büyüme düzenleyicilerinin etkinlikleri değerlendirilmiştir. Değerlendirilen tüm bazal ortamlar arasında aktif kömür içeren Orchimax en iyi çimlenme oranını (%62.46) verdi. Bu ortamın 1.0 mg/L zeatin ile desteklenmesi en yüksek çimlenme oranı (%81.18) ile sonuçlanmıştır. En düşük thidiazuron konsantrasyonunun (0.25 mg/L) sürgün uzaması üzerinde, kinetin’in (0.5 ve 1.0 mg/L) ise yaprak oluşumu üzerinde daha etkili olduğu belirlenmiştir. Yine aynı bazal besi ortamının 1.0 mg/L ve 2.0 mg/L IBA ile desteklenmesiyle sırasıyla kök uzamasını (31.16 mm) ve kök oluşumunu (fide başına 4.5 adet kök) daha fazla teşvik etmiştir. Sitokininlerin, özelliklede 2. 0 mg/L zeatin’in, yumru oluşumunu ve yumrulardaki glukomannan birikimininde daha etkili olduğu tespit edilmiştir. İn vitro koşullarda üretilen fideler kademeli bir şekilde toprak koşullarına adapte edilmiştir.

show abstract

Section: Resultssupporting

confidence: 93%

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

In vitro asymbiotic germination, seedling formation and tuberization of Orchis sancta

Bektaş

Sökmen

2022

Turkish Journal of Biodiversity

Self Cite

View full text Add to dashboard Cite

show abstract

Phytochemical Analysis of Anacamptis coriophora Plant Cultivated Using Ex Vitro Symbiotic Propagation

Aytar,

Harzli,

Özdener Kömpe

2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

This study aims to investigate the functional groups and phytochemical profile of Anacamptis coriophora seeds, tubers, and flowers. Symbiotic seedlings produced using the ex vitro method were transferred to their natural habitat and grown to analyze the functional groups and phytochemical profiles of tubers and flowers. The life cycles of the transferred seedlings were monitored, and tubers and flowers were harvested for analysis. ATR‐FTIR spectroscopy revealed the presence of functional groups such as polysaccharides, lignin, and proteins in both tubers and flowers. Differences in spectral frequencies between first‐year and second‐year tubers were observed. Fatty acid analysis identified 30 different compounds in seeds, flowers, and tubers, with linoleic acid being the most abundant (27% in seeds, 33% in tubers), and palmitic acid present in flowers (24%). GC‐MS analysis of ethanol extracts from these components highlighted the presence of 32 compounds, including hydroxyacetic acid, hydrazide, cytidine (Z)‐7‐hexadecenal, 2,2‐dimethoxyethane, 2,5,6‐trimethyldecane, and butanamide, 4‐amino‐N‐hydroxy. A. coriophora's tubers, flowers, and seeds may contain active metabolites with therapeutic potential. These results are valuable for the commercial cultivation of the plant.

show abstract

Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’ X Dendrobium ‘Blackspider X Sutiknoi’)

Wijaya

Artadana

Putra

et al. 2023

AIP Conference Proceedings

View full text Add to dashboard Cite

Dendrobium is a genus of orchids that has been widely planted, both domestically and internationally. Orchid conventional breeding is generally done to produce new variants, but seeds from crosses are generally difficult to germinate. To solve this problem, tissue culture has long been used to germinate orchid seeds. This study aims to obtain a suitable medium composition for growing seeds from crosses of Dendrobium 'Bertachong' X Dendrobium 'Blackspider x Sutiknoi'. The seeds were grown on two different media types, MS (Murashige-Skoog) and VW (Vacin-Went), with the addition of 2% sucrose and with or without the addition of organic extracts (either mungbean sprouts extract or coconut water). Plantlets were sub-cultured every 4 weeks for 2 times on the same medium but with the addition of activated charcoal, and followed by a third subculture with the addition of 1 mg.L -1 NAA (Naphthalene Acetic Acid) in each medium. The best growth shown by fast regeneration and good seedling quality (multiple buds and roots formed) was obtained on MS medium with the addition of 2% sucrose and 15% mungbean sprouts extract.

show abstract

In vitro germination, protocorm formation, and plantlet development of Orchis coriophora (Orchidaceae), a naturally growing orchid species in Turkey

Cited by 22 publications

References 24 publications

In vitro asymbiotic germination, seedling formation and tuberization of Orchis sancta

In vitro asymbiotic germination, seedling formation and tuberization of Orchis sancta

Phytochemical Analysis of Anacamptis coriophora Plant Cultivated Using Ex Vitro Symbiotic Propagation

Effect of organic additives on regeneration of orchid hybrid (Dendrobium ‘Bertachong’ X Dendrobium ‘Blackspider X Sutiknoi’)

Contact Info

Product

Resources

About