In vitro haemostatic efficacy of aqueous, methanol and ethanol plant extracts of three medicinal plant species in Palestine

Abstract

The haemostatic efficacy of different extract types of Satureja thymbra L., Thymbra spicata L. (Lamiaceae) and Verbascum fruticulosum Post. (Scrophulariaceae) was evaluated in this study via the Prothrombin time (PT) and Activated partial thromboplastin time (aPTT) analysis. Aqueous, methanol and ethanol extracts of the examined plant species leaves were prepared to a final concentration 50 mg/mL. In vitro PT and aPTT assays were conducted on normal platelet poor plasma blood samples by a digital coagulation analyzer. The obtained results revealed anticoagulation activity of all investigated plant species with observed variations among them. The aqueous and ethanol extracts of T. spicata as well as the aqueous extract of S. thymbra prolonged PT values significantly (p < 0.05). While, all V. fruticulosum extract types have had no significant effect on the PT values. The recorded aPTT data showed that all aqueous extracts have had a significant effect on the blood haemostasis as they increased aPTT values in all plant species under study. Out of which, both the ethanol and methanol extracts of T. spicata and methanol extract of S. thymbra showed similar effect. Of great concern, it was clearly noticed that the aqueous and ethanol extract of T. spicata and the aqueous extract of S. thymbra possess the strongest anticoagulation effect as they increased both PT and aPTT values significantly relative to the control (p < 0.05). The variable anticoagulation bioactivity among the studied plant species could be referred to the various solvents degrees of solubility of different phyto-constituents. Thus, the efficacy of the plant species extracts evaluation as anticoagulants or coagulants were related to the plant species and to the solvent of extraction.

Antioxidant and Anti–Aging Activity of Freeze–Dried Alcohol–Water Extracts from Common Nettle (Urtica dioica L.) and Peppermint (Mentha piperita L.) in Elastomer Vulcanizates

“…Many works associated with selected parts of the herb or plant aimed to determine the content of specific compounds responsible for required biological activity [ 18 , 19 , 20 ]. Studies on natural extracts from many plants have proven their antioxidant, antimicrobial, antifungal, antibacterial, anti-inflammatory, antipyretic and analgesic activity and, as a consequence, wide potential application [ 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ]. Among many plants with various biological effects, Mentha piperita L. and Urtica dioica L. are species with intensive antioxidant and anti-aging properties associated with the presence of various phytochemicals, such as terpenoids, carotenoids, tannins, vitamins, acids and many derivatives [ 29 , 30 , 31 ].…”

A comparative study to evaluate the effects of antibiotics, plant extracts and fluoride-based toothpaste on the oral pathogens isolated from patients with gum diseases in Pakistan

“…The rhizomes of C. longa were dried and then crushed to make powder, and 25 grams of dry powder was put into the Soxhlet apparatus for extraction of soluble bioactive molecules from the rhizome by using various solvents (like chloroform, benzene, petroleum ether, methanol and water). It was then concentrated in the rotary evaporator under low pressure as described by Omar et al (2020) and Ali et al (2020). The condensed extract was discharged to sterilized collecting tube.…”

In vitro Evaluation of Hemostatic Properties of Areal Parts Aqueous Extract of Ageratum conyzoïdes L. (Asteraceae)

“…In vitro Clotting time measurement was carried out using amodified method of Lee and White as reported these authors [17,18]. The method described by some authors was adopted in this study with slight modifications.…”

“…Coagulation was considered complete when the tube could be reversed without significant blood movement in the tube. The time required for blood coagulation was recorded in each tube and the procedure was repeated for the other tubes [18]. All data were presented as mean ± SEM.…”