In vitro identification of targeting ligands of human M cells by phage display

Fiévez, Virginie; Plapied, Laurence; Plaideau, Catheline; Legendre, Daniel; Rieux, Anne des; Pourcelle, Vincent; Freichels, Hélène; Jérôme, Christine; Marchand, Jean; Préat, Véronique; Schneider, Y.‐J.

doi:10.1016/j.ijpharm.2010.04.023

Cited by 48 publications

(35 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, gastrointestinal administration of proteins, in the absence of adjuvants, preferentially induces immunological tolerance ("oral tolerance") 1 , but adjuvant-including enteric vaccines are superior in inducing IgA responses which are important to prevent infections with 4 targeting [13][14][15] and receptor identification 16,17 . Extensive screening of phage display libraries has also been used to identify ligands that facilitate translocation across the mucosa of the gastrointestinal tract 18 or binding to and transport via M cells 19,20 or goblet cells 21,22 .…”

Section: Introductionmentioning

confidence: 99%

Identification of Targeting Peptides for Mucosal Delivery in Sheep and Mice

et al. 2015

View full text Add to dashboard Cite

In this study we identified and characterized a novel cyclic peptide that facilitates the rapid transportation of conjugated molecules across the epithelial layer of the small intestine. The peptide was initially selected from phage display libraries using a large animal experimental model which employed consecutive in vitro and in vivo panning. The procedure was designed to enrich for peptides that facilitated transcytosis across the intestinal epithelium into the intestinal afferent lymphatic system. A small set of peptides was repeatedly isolated using this selection method, 2 however the cyclic nonamer CTANSSAQC, 13C dominated. The activity of the putative targeting peptide C13 was then verified using a mouse model. These experiments showed that the 13C peptide as well as macromolecules conjugated to it were rapidly transported across the intestinal mucosa into distinct subsets of epithelial cells and CD11+ cells located in the lamina propria and Peyer's Patches. Significant amounts of intact protein could be delivered into the systemic circulation after rectal and nasal application. Thus, peptide 13C is regarded as an attractive carrier candidate for mucosal delivery of large molecules. The preferential targeting to distinct intestinal cells may be utilized to deliver active biological drugs for the effective control of diseases of the gut.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of Targeting Peptides for Mucosal Delivery in Sheep and Mice

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Table 2 gives a non exhaustive list of receptors that have been targeted to increase nanocarrier transport by intestinal cells or M cells. These receptors are either well known receptors expressed by intestinal cells or new targets that have been identified by phage display for specific and non specific translocation [68,69].…”

Section: Mechanisms Of Cellular Uptake Of Nanocarriersmentioning

confidence: 99%

“…3B). The grafting of new M cell homing peptides identified by phage display on nanoparticles is associated with increased delivery to M cells and enhanced transport [68,71]. Thiamine-coated nanoparticles show a strong capacity to be captured by Peyer's patches [23].…”

Section: Mechanisms Of Cellular Uptake Of Nanocarriersmentioning

confidence: 99%

Fate of polymeric nanocarriers for oral drug delivery

Plapied

Duhem

Rieux

et al. 2011

Current Opinion in Colloid & Interface Science

294

173

View full text Add to dashboard Cite

“…Fievez et al [110] identified M cell targeting peptide by phage display using an in vitro model of the human FAE. Among different peptide sequences, CTGKSC and LRVC sequences that occur at high frequency enhanced phage transport across M-like cells.…”

Section: Mucoadhesivementioning

confidence: 99%

Mucosal Delivery of Vaccine by M Cell Targeting Strategies

Singh¹,

Maharjan²,

Jiang³

et al. 2014

CDTH

View full text Add to dashboard Cite

Mucosal vaccination is one of the most effective methods to prevent infectious diseases because most pathogens enter the body at mucosal surfaces. Mucosal vaccination induces not only systemic immune responses but also mucosal responses compared to parenteral vaccination that induces poor mucosal immunity. Another advantages of using mucosal vaccines are high patient compliance, low cost and easy other administration. Despite these advantages, very few mucosal vaccines are commercially available today. This is because mucosal vaccines are prone to degradation in the harsh conditions of the gastrointestinal (GI) tract lowering the bioavailability of antigens to induce immune responses. Therefore, protective and effective formulations are required for successful mucosal vaccination. Accordingly, the use of nano-and micro-polymeric particles has received much attention as delivery vehicles of antigens because they can protect the antigens from degradation in the GI tract and they also enhance the antigen uptake in mucosal-associated lymphoid tissue. Particularly, mucoadhesive polymeric carriers are the most promising vehicles for mucosal vaccine delivery because these carriers retain the vaccines on the mucosal tissues for longer period thus improving the bioavailability of the antigens. Most importantly, M cells on the follicle-associated epithelium of the Peyer's patch play a key role in mucosal infection and immunity because they uptake and deliver antigens across mucosal epithelia to the lymphoid tissues via transcytosis. In this review, we dig the role and characteristics of M cells on mucosal immunization and explore the molecules of M cells for targeted delivery of antigens by polymeric particle system.

show abstract

In vitro identification of targeting ligands of human M cells by phage display

Cited by 48 publications

References 41 publications

Identification of Targeting Peptides for Mucosal Delivery in Sheep and Mice

Identification of Targeting Peptides for Mucosal Delivery in Sheep and Mice

Fate of polymeric nanocarriers for oral drug delivery

Mucosal Delivery of Vaccine by M Cell Targeting Strategies

Contact Info

Product

Resources

About