In vitro impact of laser irradiation on platelet aggregation

Shakir, E.; Naji, Nabaa A. Rasheed

doi:10.1007/s10103-018-2527-4

Cited by 5 publications

(4 citation statements)

References 22 publications

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“…To strengthen our results, it has been found that ATP release inhibits platelet aggregation [48]. Similar observations were made when laser-irradiated platelets were incubated with agonists [13,14,50]. To further support our findings, another study reported that gamma radiation could be lethal to platelet function due to the production of free radicals during long-term platelet storage that causes exposure to CD62P and affects platelet function [51].…”

Section: Discussionsupporting

confidence: 88%

X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets

Khan

Liu

Meng

et al. 2023

CIMB

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X-rays can induce morphological as well as functional changes in cells. Platelets are anuclear cellular fragments originating from megakaryocytes and are the major regulators in hemostasis and thrombosis. Platelet products are irradiated to avoid medical complications associated with platelet transfusion. So far, gamma, UV, and laser radiation have been used for this purpose. However, scientists are divided about the effects of radiation on platelet quality. The present study was designed to explore the possible effects of X-rays in washed human platelets and understand the molecular mechanism behind them. In the present study, we exposed washed human platelets to 10 or 30 Gy X-rays at 0.25 Gy/min. Flow cytometry, aggregometry, and western blot were performed to investigate the effect of X-rays on platelet degranulation, integrin activation, platelet aggregation, and apoptosis. It was found that X-rays immediately induced granular secretions with no effect on GP IIb/IIIa activation. Not surprisingly, due to granule secretions in irradiated platelets, platelet aggregation was significantly reduced. In contrast to granular secretions and platelet aggregation, X-rays induced mitochondrial transmembrane potential depolarization in a time-dependent manner to induce apoptosis and activated protein kinase C (PKC) signaling. This study revealed and explained the molecular mechanism activated by X-rays in washed human platelets. Here we also introduced Gö 6983, a PKC inhibitor, as an agent that counteracts X-ray-induced changes and maintains the integrity of platelets.

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Section: Discussionsupporting

confidence: 88%

X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets

Khan

Liu

Meng

et al. 2023

CIMB

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“…For a better understanding of concepts and effects, Table 1 presents PBM experimental studies [ 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 ].…”

Section: New Introspections and Perspectives On Photobiomodulationmentioning

confidence: 99%

“…Lasers Med Sci. 2018, 33, 1717-1721. doi:10.1007/s10103-018-2527-4 [169] In vitro blood platelets from 30 healthy volunteers 532 nm; power 100 mW; CW; 4-mm-diameter irradiation beam spot. Irradiation times: 1.8, 3.7, and 6.2 s giving doses of irradiation 1.5, 3, and 5 J/cm 2 , respectively.…”

mentioning

confidence: 99%

Molecular and Cellular Mechanisms of Arthritis in Children and Adults: New Perspectives on Applied Photobiomodulation

Ailioaie

Litscher

2020

IJMS

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Juvenile idiopathic arthritis and adult rheumatoid arthritis are two major groups with chronic joint pain and inflammation, extra-articular manifestations, and high risk of comorbidities, which can cause physical and ocular disability, as well as create great socio-economic pressure worldwide. The pathogenesis of arthritis manifested in childhood and adulthood is multifactorial, unclear, and overly complex, in which immunity plays an important role. Although there are more and more biological agents with different mechanisms of action for the treatment of arthritis, the results are not as expected, because there are partial responses or non-responsive patients to these compounds, high therapeutic costs, side effects, and so on; therefore, we must turn our attention to other therapeutic modalities. Updating knowledge on molecular and cellular mechanisms in the comparative pathogenesis of chronic arthritis in both children and adults is necessary in the early and correct approach to treatment. Photobiomodulation (PBM) represents a good option, offering cost-effective advantages over drug therapy, with a quicker, more positive response to treatment and no side effects. The successful management of PBM in arthritis is based on the clinician’s ability to evaluate correctly the inflammatory status of the patient, to seek the optimal solution, to choose the best technology with the best physical parameters, and to select the mode of action to target very precisely the immune system and the molecular signaling pathways at the molecular level with the exact amount of quantum light energy in order to obtain the desired immune modulation and the remission of the disease. Light is a very powerful tool in medicine because it can simultaneously target many cascades of immune system activation in comparison with drugs, so PBM can perform very delicate tasks inside our cells to modulate cellular dysfunctions, helping to initiate self-organization phenomena and finally, healing the disease. Interdisciplinary teams should work diligently to meet these needs by also using single-cell imaging devices for multispectral laser photobiomodulation on immune cells.

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“…Lasers operating in the visible spectral region particularly 532 nm has been widely used in medical technology such as skin rejuvenation, platelet aggregation, laryngeal lesions, acne treatment, tattoo and pigmentation control, prostate surgery, cardiovascular surgery, vascular lesions, glaucoma treatment, tissue remodeling, reduction in facial wrinkles etc. [6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Nevertheless, biological effects of laser signify it as a competent tool as well as a potentially hazardous device presenting with unforeseen occupational risks to operators and ancillary personnel [21].…”

Section: Introductionmentioning

confidence: 99%

Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors

et al. 2020

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High energy laser, particularly 532 nm, is widely used in defense and medical applications and there is need to address its occupational safety. Thermal and non-thermal effects of 532 nm high energy laser on skin are cause of concern. This study indicates impact of 532 nm laser on rat skin and first of its kind of attempt to understand transcriptional activation of genes as an early response following laser exposure. Skin of experimental rats were exposed to 532 nm radiance at 0.1, 0.25 and 0.50 W/cm 2 for 10 sec. Thermographic changes of skin exposed to 532 nm laser exhibited increased Tmax temperature in radiance dependent manner. After thermal imaging, skin of experimental rats was collected 1 h post laser exposure for studying differential gene expression. The skin exposed to lower power density (0.1 W/cm 2 ) did not show significant changes in expression of gene pathways studied. At moderate radiance (0.25 W/cm 2 ), predominantly canonical wnt/B-catenin pathway genes notch1, axin2, ccdn1, wnt5a and redox homeostasis genes; txn1, nqo1 and txnrd1 were expressed. At higher radiance (0.5 W/cm 2 ), significant repression of genes related to wound healing process particularly notch/wnt pathway viz. hes5, wnt1, wn3b with higher expression of dab2 was recorded. The data obtained from these studies would help in drawing safety limits for skin exposure to 532 nm laser. Further, genes expressed at moderate and high level of radiance exposure to skin were distinct and differential and provide new avenue to configure pathway to counteract laser induced delay in tissue injury and hair follicular damage. OPEN ACCESS Citation: Tulsawani R, Sharma P, Sethy NK, Kumari P, Ganju L, Prakash S, et al. (2020) Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors. PLoS ONE 15(3): e0230175. https://doi.org/10.Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors PLOS ONE | https://doi.org/10.Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors PLOS ONE | https://doi.org/10.1371/journal.pone.0230175 March 19, 2020 3 / 21 Fig 4. Volcano plot of genes expressed following 532 nm laser exposure at moderate irradiance of 0.25 W/cm 2 . https://doi.org/10.1371/journal.pone.0230175.g004 PLOS ONEAcute exposure of 532 nm laser differentially regulates skin tissue transcription factors PLOS ONE | https://doi.org/10.

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In vitro impact of laser irradiation on platelet aggregation

Cited by 5 publications

References 22 publications

X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets

X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets

Molecular and Cellular Mechanisms of Arthritis in Children and Adults: New Perspectives on Applied Photobiomodulation

Acute exposure of 532 nm laser differentially regulates skin tissue transcription factors

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