In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods

Guo, Xiling; Chen, Yin; Wang, Ling; Wu, Xiaosong; jingjing, Fan; Li, Fang; xiaoyan, Zeng; Ge, Yiyue; ying, Chi; Cui, Lunbiao; Liu-bo, Zhang; Sun, Han‐Dong; Yan, Xu

doi:10.21203/rs.3.rs-43489/v1

Cited by 10 publications

(10 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While only a single device was tested in this study, a variety of UV inactivation studies have been published focusing on SARS-CoV-2 and other coronaviruses using different wavelengths and environmental conditions [ 30 , 31 , 41 , 42 , 43 , 44 , 45 ]. In contrast to the broad-spectrum UV device tested here, most UV devices that have been tested for efficacy against SARS-CoV-2 produce constant, non-pulsed UV-C (222–300 nm) light exclusively [ 28 , 30 , 31 , 32 , 33 ]. One other study has focused on a UV device that produces pulsed UV from high intensity xenon lamps; however, the wavelengths produced by the device were not reported [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…While chemical disinfectants and alcohols are effective methods of inactivating SARS-CoV-2 in most circumstances, disinfection of large spaces using these methods is a laborious process requiring close contact with potentially contaminated surfaces [ 25 , 26 , 27 , 28 ]. UV light has long been established as an effective and direct method for the inactivation of enveloped viruses [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

“…The UV spectrum is divided into three sub-spectra: UV-A (315–400 nm), UV-B (280–315 nm), and UV-C (100–280 nm). Most current studies that have focused on the efficacy of UV light in inactivating SARS-CoV-2 utilize devices that produce solely UV-C light [ 28 , 30 , 31 , 32 , 33 ]. UV-C light is the most common UV sub-spectra produced by commercially available UV disinfection devices due to its well-known germicidal properties [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Pulsed Broad-Spectrum UV Light Effectively Inactivates SARS-CoV-2 on Multiple Surfaces and N95 Material

Jureka

Williams

Basler

2021

Viruses

View full text Add to dashboard Cite

The ongoing SARS-CoV-2 pandemic has resulted in an increased need for technologies capable of efficiently disinfecting public spaces as well as personal protective equipment. UV light disinfection is a well-established method for inactivating respiratory viruses. Here, we have determined that broad-spectrum, pulsed UV light is effective at inactivating SARS-CoV-2 on multiple surfaces in vitro. For hard, non-porous surfaces, we observed that SARS-CoV-2 was inactivated to undetectable levels on plastic and glass with a UV dose of 34.9 mJ/cm2 and stainless steel with a dose of 52.5 mJ/cm2. We also observed that broad-spectrum, pulsed UV light is effective at reducing SARS-CoV-2 on N95 respirator material to undetectable levels with a dose of 103 mJ/cm2. We included UV dosimeter cards that provide a colorimetric readout of UV dose and demonstrated their utility as a means to confirm desired levels of exposure were reached. Together, the results presented here demonstrate that broad-spectrum, pulsed UV light is an effective technology for the in vitro inactivation of SARS-CoV-2 on multiple surfaces.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pulsed Broad-Spectrum UV Light Effectively Inactivates SARS-CoV-2 on Multiple Surfaces and N95 Material

Jureka

Williams

Basler

2021

Viruses

View full text Add to dashboard Cite

show abstract

“…Therefore, contaminated surfaces and solutions are a reservoir for transmission through fomites, meaning effective hygiene and environmental decontamination is crucial in helping to prevent the spread of COVID-19 [11,12]. Disinfectant solutions of 75 % ethanol and 10 % sodium hypochlorite are able to reduce SARS-CoV-2 titre by at least 2.0 log 10 TCID 50 ml −1 and 3.25 log 10 TCID 50 ml −1 , respectively, within 5 min [10,13]. However, the World Health Organization (WHO) has recommended diluting household bleach 1 : 100 to reduce irritation to the user and contact times of 10 to 60 min to disinfect surfaces and when immersing items [14].…”

Section: Discussionmentioning

confidence: 99%

Inactivation of SARS-CoV-2 on surfaces and in solution with Virusend (TX-10), a novel disinfectant

Anderson

Hughes

Patterson

2021

Access Microbiology

View full text Add to dashboard Cite

Until an effective vaccine against SARS-CoV-2 is available on a widespread scale, the control of the COVID-19 pandemic is reliant upon effective pandemic control measures. The ability of SARS-CoV-2 to remain viable on surfaces and in aerosols, means indirect contact transmission can occur and there is an opportunity to reduce transmission using effective disinfectants in public and communal spaces. Virusend (TX-10), a novel disinfectant, has been developed as a highly effective disinfectant against a range of microbial agents. Here we investigate the ability of Virusend to inactivate SARS-CoV-2. Using surface and solution inactivation assays, we show that Virusend is able to reduce SARS-CoV-2 viral titre by 4 log10 p.f.u. ml−1 within 1 min of contact. Ensuring disinfectants are highly effective against SARS-CoV-2 is important in eliminating environmental sources of the virus to control the COVID-19 pandemic.

show abstract

“…van Doremalen et al (2020) found the SARS-COV-2 viruses on the outer surface of a face mask for up to 7 days [10]. According to WHO's interim guidance to clean and disinfect environment in the context of SARS-COV-2 virus, disinfectant products containing alcohol 70 -90%, chlorine-based at 0.1% v/v and 0.5% v/v and hydrogen peroxide at > 0.5% v/v can be used to combat SARS-COV-2 virus transmission [11,12]. Since no report was found about the influence of the disinfection step on recycled plastic pellets' physical and chemical properties, we report the physicochemical characteristic of the filtering layer from disposable mask before and after the recycling process.…”

Section: Introductionmentioning

confidence: 99%

The Physicochemical Characteristics of Recycled-Plastic Pellets Obtained From Disposable Face Mask Wastes

Saraswaty

Nissa

Firdiana

et al. 2021

jsmi

View full text Add to dashboard Cite

THE PHYSICOCHEMICAL CHARACTERISTICS OF RECYCLED-PLASTIC PELLETS OBTAINED FROM DISPOSABLE FACE MASK WASTES. The government policy to wear a face mask during the COVID-19 pandemic has increased disposable face mask wastes. Thus, to reduce such wastes, it is necessary to evaluate the physicochemical characteristics of disposable face masks wastes before the recycling process and the recycled products. In this study, physicochemical characterization of the 3-ply disposable face masks and the recycled plastic pellets after disinfection using 0.5% v/v sodium hypochlorite were evaluated. A set of parameters including the characterization of surface morphology by a scanning electron microscope (SEM), functional groups properties by a fourier transform infra-red spectroscopy (FT-IR), thermal behavior by a differential scanning calorimetry (DSC), tensile strength and elongation at break were evaluated. The surface morphological of each layer 3-ply disposable face mask showed that the layers were composed of non-woven fibers. The FT-IR evaluation revealed that 3-ply disposable face mask was made from a polypropylene. At the same time, the DSC analysis found that the polypropylene was in the form of homopolymer. The SEM analysis showed that the recycled plastic pellets showed a rough and uneven surface. The FT-IR, tensile strength and elongation at break of the recycled plastic pellets showed similarity with a virgin PP type CP442XP and a recycled PP from secondary recycling PP (COPLAST COMPANY). In summary, recycling 3-ply disposable face mask wastes to become plastic pellets is recommended for handling disposable face mask wastes problem.

show abstract

In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods

Cited by 10 publications

References 12 publications

Pulsed Broad-Spectrum UV Light Effectively Inactivates SARS-CoV-2 on Multiple Surfaces and N95 Material

Pulsed Broad-Spectrum UV Light Effectively Inactivates SARS-CoV-2 on Multiple Surfaces and N95 Material

Inactivation of SARS-CoV-2 on surfaces and in solution with Virusend (TX-10), a novel disinfectant

The Physicochemical Characteristics of Recycled-Plastic Pellets Obtained From Disposable Face Mask Wastes

Contact Info

Product

Resources

About