In vitro induction of polyploidy by colchicine treatment in herbaceous peony (<i>Paeonia lactiflora</i> Pall.)

Wei, Dongfeng; Tang, Zhengjiao; Silva, J. A. T. da; Yu, Xiaonan

doi:10.17660/actahortic.2017.1171.35

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…All these limitations restrict the development of the above breeding techniques. Therefore, their effectiveness has been very limited 11,12,105,106 .…”

Section: Traditional Breedingmentioning

confidence: 99%

Germplasm resources and genetic breeding of Paeonia: a systematic review

Yang

Sun

et al. 2020

Hortic Res

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Members of the genus Paeonia, which consists of globally renowned ornamentals and traditional medicinal plants with a rich history spanning over 1500 years, are widely distributed throughout the Northern Hemisphere. Since 1900, over 2200 new horticultural Paeonia cultivars have been created by the discovery and breeding of wild species. However, information pertaining to Paeonia breeding is considerably fragmented, with fundamental gaps in knowledge, creating a bottleneck in effective breeding strategies. This review systematically introduces Paeonia germplasm resources, including wild species and cultivars, summarizes the breeding strategy and results of each Paeonia cultivar group, and focuses on recent progress in the isolation and functional characterization of structural and regulatory genes related to important horticultural traits. Perspectives pertaining to the resource protection and utilization, breeding and industrialization of Paeonia in the future are also briefly discussed.

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“…All these limitations restrict the development of the above breeding techniques. Therefore, their effectiveness has been very limited 11,12,105,106 .…”

Section: Traditional Breedingmentioning

confidence: 99%

Germplasm resources and genetic breeding of Paeonia: a systematic review

Yang

Sun

et al. 2020

Hortic Res

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“…During past few decades, arti cial polyploidization has played a key role in plant breeding and crop improvement. The improvement in agronomic traits was possible in polyploids due to their superiority in physiological and morphological tness compared to their natural diploids (Sattler et al 2016;Wei et al 2017). Polyploids often exhibit improved agronomic traits such as increased vigor, improved productivity, enhanced tolerance to environmental stresses, pest and pathogens, higher metabolite production as well as restoration of fertility in wide hybrids (Javadian et al 2017).…”

Section: Introductionmentioning

confidence: 99%

In vitro induction and assessment of tetraploid plants from shoot cultures of diploid Niger (Guizotia abyssinica (L.f.) Cass.): a multipurpose oilseed crop

Chambhare

Nikam

2021

Plant Cell Tiss Organ Cult

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Agronomic traits improvement in crop plant can be accomplished by induction of polyploidy. Niger (Guizotia abyssinica (L.f.) Cass.) is one of the important edible oil yielding diploid crop (2n = 30). In the present study, the tetraploidization in Niger plants was achieved by treating apical portion of in vitro raised shoots with colchicine and their con rmation by chromosome counting and ow cytometry. The in vitro shoots were raised from leaf explants on MS medium supplemented with 1.0 mg/l BAP (6benzylaminopurine). The survival and nature of growth of treated shoots was variable with colchicine concentration (0.0, 0.005, 0.01, 0.02, 0.03, and 0.04%) and exposure time (4, 8, 12, and 16 h). The maximum tetraploid induction ratio was recorded with 0.02% colchicine treatment for 8 h which yielded 38.4% tetraploids. The chromosome number in root cells of tetraploid plantlets was 2n=4x=60 and the DNA content in leaf cells was 10.34 pg which was double to the diploid plant (4.70 pg) cells. In addition, there was signi cant difference exist in leaf characteristics of diploid and tetraploid plantlets. Compared to diploid plantlets, the tetraploid plantlets showed larger leaves, larger stomatal size, low stomatal index, larger capitula, larger seeds, and a greater number of seeds per capitula. The oil content in seeds was higher and associated with altered fatty acid pro le. The results demonstrated that the tetraploid plantlets obtained in this study exhibited some superior agronomical traits (as mentioned above) compared to diploid. The developed protocol and produced tetraploid plantlets will open the new door in improvement of edible oil yielding crop Guizotia abyssinica (L.f.) Cass.

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“…As a result of this historical cultural affinity, the ornamental value of Paeonia is extremely high (Liu et al 1987), resulting in a considerable body of literature and research originating from China (Yang et al 2017). Despite this, the cultivation of peony and Paeonia cut flowers is also popular internationally (Wei et al 2017a). Charles Darwin, in his 'Origin of the Species' which was published in 1859, cited an example of Chinese peony breeding with the subsequent creation of many new cultivars under artificial cultivation to exemplify the theory of the creation of natural versus artificial selection (Darwin 2009).…”

Section: Introductionmentioning

confidence: 99%

Advances in molecular biology of Paeonia L.

Fan

Wang

Dong

et al. 2019

Planta

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Molecular biology can serve as a tool to solve the limitations of traditional breeding and cultivation techniques related to flower patterns, the improvement of flower color, and the regulation of flowering and stress resistance. These characteristics of molecular biology ensured its significant role in improving the efficiency of breeding and germplasm amelioration of Paeonia. This review describes the advances in molecular biology of Paeonia, including: (1) the application of molecular markers; (2) genomics, transcriptomics, proteomics, metabolomics, and microRNA studies; (3) studies of functional genes; and (4) molecular biology techniques. This review also points out select limitations in current molecular biology, analyzes the direction of Paeonia molecular biology research, and provides advice for future research objectives.

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In vitro induction of polyploidy by colchicine treatment in herbaceous peony (Paeonia lactiflora Pall.)

Cited by 5 publications

References 6 publications

Germplasm resources and genetic breeding of Paeonia: a systematic review

Germplasm resources and genetic breeding of Paeonia: a systematic review

In vitro induction and assessment of tetraploid plants from shoot cultures of diploid Niger (Guizotia abyssinica (L.f.) Cass.): a multipurpose oilseed crop

Advances in molecular biology of Paeonia L.

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