In vitro Infection of Human Fetal Membranes with Candida Species

Gürgan, Timur; Diker, Kadir Serdar; Haziroğlu, Rıfkı; Urman, Bülent; Akan, Mehmet

doi:10.1159/000292549

Cited by 21 publications

(11 citation statements)

References 7 publications

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“…In a study addressed to know whether Candida species can penetrate intact fetal membranes under ex vivo conditions, it was possible to demonstrate that C. albicans was the only one that, once inoculated into the maternal side, penetrated and passed to the fetal side, and caused degeneration of the structure of the membrane epithelium [46]. …”

Section: Discussionmentioning

confidence: 99%

Tissue-specific human beta-defensins (HBD)-1, HBD-2 and HBD-3 secretion profile from human amniochorionic membranes stimulated with Candida albicans in a two-compartment tissue culture system

Zaga‐Clavellina

Ruiz

Flores-Espinosa

et al. 2012

Reprod Biol Endocrinol

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BackgroundDuring intrauterine infection, amniochorionic membranes represent a mechanical and immunological barrier against dissemination of infection. Human beta defensins (HBD)-1, HBD-2, and HBD-3 are key elements of innate immunity that represent the first line of defense against different pathogen microorganisms associated with preterm labor. The aim of this work was to characterize the individual contribution of the amnion (AMN) and choriodecidua (CHD) regions to the secretion of HBD-1, HBD-2 and HBD-3, after stimulation with Candida albicans.MethodsFull-thickness human amniochorionic membranes were obtained after delivery by elective cesarean section from women at 37-40 wk of gestation with no evidence of active labor. The membranes were cultured in a two-compartment experimental model in which the upper compartment is delimited by the amnion and the lower chamber by the choriodecidual membrane. One million of Candida albicans were added to either the AMN or the CHD face or to both and compartmentalized secretion profiles of HBD-1, HBD-2, and HBD-3 were quantified by ELISA. Tissue immunolocalization was performed to detect the presence of HBD-1, -2, -3 in tissue sections stimulated with Candida albicans.ResultsHBD-1 secretion level by the CHD compartment increased 2.6 times (27.30 [20.9-38.25] pg/micrograms protein) when the stimulus with Candida albicans was applied only on this side of the membrane and 2.4 times (26.55 [19.4-42.5] pg/micrograms protein) when applied to both compartments simultaneously. HBD-1 in the amniotic compartment remained without significant changes. HBD-2 secretion level increased significantly in the CHD when the stimulus was applied only to this region (2.49 [1.49-2.95] pg/micrograms protein) and simultaneously to both compartments (2.14 [1.67- 2.91] pg/micrograms protein). When the stimulus was done in the amniotic compartment HBD-2 remained without significant changes in both compartments. HBD-3 remained without significant changes in both compartments regardless of the stimulation modality. Localization of immune-reactive forms of HBD-1, HBD-2, and HBD-3 was carried out by immunohistochemistry confirming the cellular origin of these peptides.ConclusionSelective stimulation of amniochorionic membranes with Candida albicans resulted in tissue-specific secretion of HBD-1 and HBD-2, mainly in the CHD, which is the first region to become infected during an ascending infection.

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Section: Discussionmentioning

confidence: 99%

Tissue-specific human beta-defensins (HBD)-1, HBD-2 and HBD-3 secretion profile from human amniochorionic membranes stimulated with Candida albicans in a two-compartment tissue culture system

Zaga‐Clavellina

Ruiz

Flores-Espinosa

et al. 2012

Reprod Biol Endocrinol

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“…C. glabrata and C. tropicalis have been demonstrated in ex vivo models to be unable to traverse intact membranes, but have been noted to cause intraamniotic infection with intact membranes [26]. b.…”

Section: Hematogenous Spreadmentioning

confidence: 99%

Intra amniotic candidiasis. Case report and meta-analysis of 54 cases

Roqué

Abdelhak²,

Young³

1999

Journal of Perinatal Medicine

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We present a case of mid pregnancy loss with retained intrauterine contraceptive device associated with fetal Candida infection. Review of English literature identified 53 additional cases of fetal candidal infection, with 17 associated with an IUCD in situ. The presence of an IUCD was associated with delivery at a statistically significant earlier gestational age when compared to cases not associated with an IUCD (23.3 +/- 4.9 vs 31.6 +/- 7.0, p < 0.001). Seventy-seven percent of fetal candidal infections associated with an IUCD were systemic (heart, brain, liver, gastrointestinal, lung) compared to 33% of cases not associated with an IUCD. In contrast to bacterial intraamniotic infections there was a low incidence of maternal febrile morbidity. An hypothesis as to the pathogenesis of Candidal infections in the presence and absence of an IUCD is offered as well as a paradigm for the management of the gravid patient with an IUCD in situ.

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“…Laboratory experiments have demonstrated that C. albicans can readily infect and invade the fetal membranes in both blastospore and hyphal forms, although other Candida species studied, including C. glabrata, were not able to do so in vitro (8). Our report clearly demonstrates the ability of C. glabrata to cause a severe chorioamnionitis resulting in poor outcome.…”

Section: Discussionmentioning

confidence: 60%

Intrauterine fetal loss associated with candida glabrata chorioamnionitis: report of two cases

Özer¹,

Unlu²,

Erşen³

et al. 2013

TJPATH

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Chorioamnionitis is most commonly the result of an ascending infection caused by bacteria found within the lower genital tract. Yeast infections causing chorioamnionitis are very uncommon. Candida glabrata is a yeast that is considered to be a commensal of the vagina but vaginitis and rarely upper genital tract infection have been described. We report two cases of fungal chorioamnionitis occurring in pregnancies with a history of in vitro fertilization and cervical cerclage, both resulting in fetal loss. The histological features in conjunction with the positive cultures enabled C. glabrata to be identified as the causative organism producing severe chorioamnionitis. C. glabrata was probably introduced into the cervix at the time of embryo transfer, and during stitching the cervix. To prevent unfavorable outcomes in pregnant women, we recommend that vaginal and in particular cervical swabs should be taken prior to cervical procedures and appropriate treatment should be provided. Key Words: Candida glabrata, Chorioamnionitis, Cervical cerclage, Fertilization in vitro ÖZKoryoamniyonitislerin büyük kısmı alt genital sistemde bulunan bakterilerin asendan enfeksiyonu sonucu gelişir. Mantar nedenli koryoamniyonitis enderdir. Kandida glabrata, vajende kommensal olarak bulunmakla birlikte nadiren vajinit ve üst genital sistem enfeksiyonuna yol açabilmektedir. Burada, in vitro fertilizasyon ile elde edilmiş, servikal serklaj uygulanmış ve fetal kayıp ile sonlanmış iki gebelikte saptanan fungal koryoamniyonitis olguları sunulmuştur. Histolojik özellikler ve pozitif kültür sonuçları ile koryoamniyonitis etkeni Kandida glabrata olarak saptanmıştır. Etkenin, embriyo transferi ve/veya servikal serklaj işlemi sırasında servikse ekilmiş olması muhtemeldir. Gebelere uygulanacak servikal işlemlerden önce vaginal ve özellikle servikal temizliğin sağlandığından emin olmak ve tedaviyi uygun planlamak bu tip istenmeyen sonuçları engelleyebilir.

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In vitro Infection of Human Fetal Membranes with Candida Species

Cited by 21 publications

References 7 publications

Tissue-specific human beta-defensins (HBD)-1, HBD-2 and HBD-3 secretion profile from human amniochorionic membranes stimulated with Candida albicans in a two-compartment tissue culture system

Tissue-specific human beta-defensins (HBD)-1, HBD-2 and HBD-3 secretion profile from human amniochorionic membranes stimulated with Candida albicans in a two-compartment tissue culture system

Intra amniotic candidiasis. Case report and meta-analysis of 54 cases

Intrauterine fetal loss associated with candida glabrata chorioamnionitis: report of two cases

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