In vitro inhibition of glucose gastro‐intestinal enzymes and antioxidant activity of hydrolyzed collagen peptides from different species

Abstract

The growing value of industrial collagen by‐products has given rise to interest in extracting them from different species of animals. Intrinsic protein structure variation of collagen sources and its hydrolysis can bring about different bioactivities. This study aimed to characterize and evaluate the differences in vitro biological potential of commercial bovine (BH), fish (FH), and porcine hydrolysates (PH) regarding their antioxidant and hypoglycemic activities. All samples showed percentages above 90% of protein content, with high levels of amino acids (glycine, proline, and hydroxyproline), responsible for the specific structure of collagen. The BH sample showed a higher degree of hydrolysis (DH) (8.7%) and a higher percentage of smaller than 2 kDa peptides (74.1%). All collagens analyzed in vitro showed inhibition of pancreatic enzymes (α‐amylase and α‐glucosidase), with the potential to prevent diabetes mellitus. The PH sample showed higher antioxidant activities measured by ORAC (67.08 ± 4.23 μmol Trolox Eq./g) and ABTS radical scavenging (65.69 ± 3.53 μmol Trolox Eq./g) methods. For the first time, DNA protection was analyzed to hydrolyzed collagen peptides, and the FH sample showed a protective antioxidant action to supercoiled DNA both in the presence (39.51%) and in the absence (96.36%) of AAPH (reagent 2,2′‐azobis(2‐amidinopropane)). The results confirmed that the source of native collagen reflects on the bioactivity of hydrolyzed collagen peptides, probably due to its amino acid composition. Practical applications Our data provide new application for collagen hydrolysates with hypoglycemiant and antioxidant activity. These data open discussion for future studies on the additional benefits arising from collagen peptide consumption for the prevention of aging complications or hyperglycemic conditions as observed in chronic diseases such as diabetes mellitus type II (DM 2). The confirmation of these results can open new market areas for the use of collagen with pharmacological applications or to produce new supplements. Furthermore, provides a solution for waste collagen from meat industries and adds value to the product.

The Potential of Bioactive Fish Collagen Oligopeptides against Hydrogen Peroxide-Induced NIH/3T3 and HUVEC Damage: The Involvement of the Mitochondria

“…Consistent with our results, Baskaran et al demonstrated that collagen peptides from Taiwan tilapia skin could increase the cell proportion in the G1 and S phases in different lung cells [37]. Furthermore, the DNA protective effects of collagen peptides derived from different species including fish have been investigated by several research teams [30,38].…”

Analgesic Efficacy of Collagen Peptide in Knee Osteoarthritis: A Meta-Analysis of Randomized Controlled Trials

“…Multiple studies demonstrated possible cellular mechanisms of the bene cial effects of collagen peptides on alleviating pain and improving joint condition in knee osteoarthritis. These mechanisms include the anti-in ammatory and antioxidant capacities of collagen peptides, and its ability to stimulate collagen synthesis and promote bone formation [22][23][24]. In vitro and in vivo studies showed a reduction of pro-in ammatory cytokines, including IL-1β, IL-6, and TNF-α after collagen peptide administration [22][25] [22].…”

“…In vitro and in vivo studies showed a reduction of pro-in ammatory cytokines, including IL-1β, IL-6, and TNF-α after collagen peptide administration [22][25] [22]. Collagen peptide also displays antioxidant activities measured by oxygen radical absorbance capacity and radical scavenging assay [24]. Furthermore, evidence has shown that orally administered collagen hydrolysate stimulates a signi cant increase in type II collagen synthesis by chondrocytes [26].…”

Analgesic efficacy of collagen peptide in knee osteoarthritis: a meta-analysis of randomized controlled trials

“…In vitro and in vivo studies showed a reduction of pro-inflammatory cytokines, including IL-1β, IL-6, and TNF-α after collagen peptide administration [ 22 , 25 ]. Collagen peptide also displays antioxidant activities measured by oxygen radical absorbance capacity and radical scavenging assay [ 24 ]. Furthermore, evidence has shown that orally administered collagen hydrolysate stimulates a significant increase in type II collagen synthesis by chondrocytes [ 26 ].…”