In Vitro Inhibition of
            <i>Streptococcus mutans</i>
            Biofilm Formation on Hydroxyapatite by Subinhibitory Concentrations of Anthraquinones

Coenye, Tom; Honraet, Kris; Rigole, Petra; Nadal‐Jimenez, Pol; Nelis, Hans

doi:10.1128/aac.00999-06

Cited by 51 publications

(35 citation statements)

References 24 publications

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“…Dental plaque is caused by biofilm formation of microorganisms on the tooth surface which exhibits several properties including reduced susceptibility to antibacterial agents (Loesohe, 1986). S. mutans was classified as the primary bacteria causing dental plaque so that prevention of biofilm formation by S. mutans could reduce the incidence of tooth decay and periodontal diseases (Coenye et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Synergistic interaction and mode of action ofCitrus hystrixessential oil against bacteria causing periodontal diseases

Wongsariya¹,

Phanthong²,

Bunyapraphatsara³

et al. 2013

Pharmaceutical Biology

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Context: Citrus hystrix de Candolle (Rutaceae), an edible plant regularly used as a food ingredient, possesses antibacterial activity, but there is no current data on the activity against bacteria causing periodontal diseases. Objective: C. hystrix essential oil from leaves and peel were investigated for antibiofilm formation and mode of action against bacteria causing periodontal diseases. Materials and methods: In vitro antibacterial and antibiofilm formation activities were determined by broth microdilution and time kill assay. Mode of action of essential oil was observed by SEM and the active component was identified by bioautography and GC/MS. Results and discussion: C. hystrix leaves oil exhibited antibacterial activity at the MICs of 1.06 mg/mL for P. gingivalis and S. mutans and 2.12 mg/mL for S. sanguinis. Leaf oil at 4.25 mg/ mL showed antibiofilm formation activity with 99% inhibition. The lethal effects on P. gingivalis were observed within 2 and 4 h after treated with 4 Â MIC and 2 Â MIC, respectively. S. sanguinis and S. mutans were completely killed within 4 and 8 h after exposed to 4 Â MIC and 2 Â MIC of oil. MICs of tested strains showed 4 times reduction suggesting synergistic interaction of oil and chlorhexidine. Bacterial outer membrane was disrupted after treatment with leaves oil. Additionally, citronellal was identified as the major active compound of C. hystrix oil. Conclusions: C. hystrix leaf oil could be used as a natural active compound or in combination with chlorhexidine in mouthwash preparations to prevent the growth of bacteria associated with periodontal diseases and biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Synergistic interaction and mode of action ofCitrus hystrixessential oil against bacteria causing periodontal diseases

Wongsariya¹,

Phanthong²,

Bunyapraphatsara³

et al. 2013

Pharmaceutical Biology

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“…Natural products and derivatives, such as green tea catechins (Hirasawa et al 2006), cranberry constituents , Galla chinensis (Xie et al 2008), citrus lemon oil (Liu et al 2013), mushroom extracts (Yano et al 2010), and several bioactive macromolecules (Shang et al 2014;Lee and Park 2015), have been reported to be effective against the growth and adherence of S. mutans. In addition, small molecules, such as tt-farnesol (Koo et al 2003), walkmycin C (Eguchi et al 2011), and anthraquinones (Coenye et al 2007), have been shown to inhibit the growth of cariogenic bacteria, and even the development of caries, but few of these compounds target S. mutans biofilms. Therefore, there is an urgent need to identify new approaches capable of targeting cariogenic bacteria in the biofilm state.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative

Chen

Ren

Zhou

et al. 2015

Appl Microbiol Biotechnol

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Dental caries, a biofilm-related oral disease, is a result of disruption of the microbial ecological balance in the oral environment. Streptococcus mutans, which is one of the primary cariogenic bacteria, produces glucosyltransferases (Gtfs) that synthesize extracellular polysaccharides (EPSs). The EPSs, especially water-insoluble glucans, contribute to the formation of dental plaque, biofilm stability, and structural integrity, by allowing bacteria to adhere to tooth surfaces and supplying the bacteria with protection against noxious stimuli and other environmental attacks. The identification of novel alternatives that selectively inhibit cariogenic organisms without suppressing oral microbial residents is required. The goal of the current study is to investigate the influence of an oxazole derivative on S. mutans biofilm formation and the development of dental caries in rats, given that oxazole and its derivatives often exhibit extensive and pharmacologically important biological activities. Our data shows that one particular oxazole derivative, named 5H6, inhibited the formation of S. mutans biofilms and prevented synthesis of extracellular polysaccharides by antagonizing Gtfs in vitro, without affecting the growth of the bacteria. In addition, topical applications with the inhibitor resulted in diminished incidence and severity of both smooth and sulcal surface caries in vivo with a lower percentage of S. mutans in the animals' dental plaque compared to the control group (P<0.05). Our results showed that this oxazole derivative has the capacity to inhibit biofilm formation and cariogenicity of S. mutans.

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“…The TC content in the films was optimum for antimicrobial activity. It is known that the MIC of the TC against Streptococcus mutans is 16 μg/ml (19). The normal saliva volume is 0.7 ml (20).…”

Section: Determination Of Tc Contentmentioning

confidence: 99%

Formulation and Evaluation of Fast Dissolving Films for Delivery of Triclosan to the Oral Cavity

2008

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Abstract. The present investigation was undertaken with the objective of formulating TC containing fast dissolving films for local delivery to oral cavity. Various film forming agents, film modifiers and polyhydric alcohols were evaluated for optimizing the composition of fast dissolving films. The potential of poloxamer 407 and hydroxypropyl-β-cyclodextrin (HPBCD) to improve solubility of TC was investigated. Fast dissolving films containing hydroxypropyl methylcellulose (HPMC), xanthan gum, and xylitol were formulated. Use of poloxamer 407 and HPBCD resulted in significant improvement in the solubility of TC. Fast dissolving films containing TC-HPBCD complex and TC-Poloxamer 407 were formulated and were evaluated for the in vitro dissolution profile and in vitro microbiological assay. Films containing TC-Poloxamer 407 exhibited better in vitro dissolution profile and in vitro antimicrobial activity as compared to the films containing TC-HPBCD complex. Effect of incorporation of eugenol on the in vivo performance of TC-Poloxamer 407 containing films was evaluated in human volunteers. Eugenol containing films improved the acceptability of TC-Poloxamer 407 films with respect to taste masking and mouth freshening without compromising the in vivo dissolution time.

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In Vitro Inhibition of Streptococcus mutans Biofilm Formation on Hydroxyapatite by Subinhibitory Concentrations of Anthraquinones

Cited by 51 publications

References 24 publications

Synergistic interaction and mode of action ofCitrus hystrixessential oil against bacteria causing periodontal diseases

Synergistic interaction and mode of action ofCitrus hystrixessential oil against bacteria causing periodontal diseases

Inhibition of Streptococcus mutans biofilm formation, extracellular polysaccharide production, and virulence by an oxazole derivative

Formulation and Evaluation of Fast Dissolving Films for Delivery of Triclosan to the Oral Cavity

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