In vitro inhibition of influenza A virus infection by marine microalga-derived sulfated polysaccharide p-KG03

The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity

“…In this study, three sampleadding modes were designed to determine the antiviral activity of sulfated polysaccharides in vitro. The antiviral mechanism of sulfated polysaccharides may be that in pre-adding polysaccharides mode, similar to clinical preventive administration [32], the polyanions of sulfated polysaccharide can combine with positive charge on the surface of recipient cells thus disturb the adsorption of virus [33]; in post-adding polysaccharide mode, equal to clinical remedial administration [34], the polyanions can block up the synthesis of viral protein thus affect transmembrane and intracellular signal transduction when virus express [35], and in simultaneously adding polysaccharide and virus after mixed mode, equal to clinical disinfection [36], the strong negative charges of polyanions can directly kill virus or combine with virus molecule thereby obstruct the virus adsorption or inhibit the reverse transcriptase of virus [37].…”

In Vitro Anti-Influenza Virus Activities of a New Lignan Glycoside from the Latex of Calotropis gigantea

“…The cells were washed with phosphate buffer saline (PBS) then incubated with viruses [including A/PR/8/34 (H1N1), A/FM/1/47 (H1N1) and A/Aichi/2/68 (H3N2)] diluted in serum-free MEM containing 100 U/mL of penicillin and 0.1 mg/mL of streptomycin for 2 h at 34°C at the multiplicities of infection (MOI = 0.01). After viral adsorption, cell monolayer was covered with overlay medium of compound 1 and further cultured at 34°C under 5% CO 2 for 48 h. Then, the overlay medium was removed, and the cell monolayer was fixed with 10% formalin, stained with 1% crystal violet, and plaques were counted [23].…”

“…Then, compound 1 (134.3 µM) or oseltamivir (6.4 µM) were added to cells at 0 h, 2 h, 4 h, 6 h, 8 h and 10 h after infection. The supernatants were harvested at 12 h post-infection and virus titers were determined by CPE and real time PCR assay [23].…”

Bioactive Compounds Derived from Microalgae Showing Antimicrobial Activities

“…Considering the present situation the discovery of antiviral compounds which were derived from algal bioactive compounds provide us a great relief. These compounds, which are tabulated in Table 2 [25][26][27][28] has a great prospective in the future.…”