The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity

Zhang, Jing; Hu, Yuanliang; Wang, Deyun; Qin, Tao; Liu, Cui; Liu, Xu; Sheng, Xin; Chang, Shanshan; Fan, Yunpeng; Li-wei, Guo

doi:10.1016/j.ijbiomac.2012.06.023

Cited by 31 publications

(11 citation statements)

References 29 publications

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“…The precipitations were lyophilized to get crude JPS t and JPS 50 . The extractions of OPS t and OPS 80 were similar to the above-mentioned method [15]. The carbohydrate contents of OPS 80 , OPS t , JPS 50 and JPS t were 80%, 79%, 52%, 51% in turn measured by the phenol-sulfuric acid method [16].…”

Section: Preparation Of Sops and Sjpsmentioning

confidence: 77%

Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide

Zhang¹,

Chen²,

Wang³

et al. 2013

International Journal of Biological Macromolecules

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Section: Preparation Of Sops and Sjpsmentioning

confidence: 77%

Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide

Zhang¹,

Chen²,

Wang³

et al. 2013

International Journal of Biological Macromolecules

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“…Total polysaccharide and protein content were measured by the phenolsulfuric acid (at 490 nm) and Bradfrod (at 595 nm) method. [5] Sulfated modification of expolysaccharide Experimental design 13 conditions were designed according to the table 1 with three factors, including CSA/Pyr ratio (v/v), temperature ( o C) and time (h) [15]. Preparation of sulfation reagent CSA was added dropwise into Pyr filled in three-necked flask, with stirring and cooling in an ice water bath [2,14].…”

Section: Preparation Of Exopolysaccharidementioning

confidence: 99%

“…The sulfur content of S-EPS was determined by the method of Terho and Hartiala [16], with sodium sulfate as a standard. The DS was calculated according to the equation [15,17] Where, S% is the sulfur content of sulfated derivatives. For Fourier Transform Infrared Spectroscopy (FT-IR) analysis, samples were recorded on a Perkin-Elmer Spectrum One Spectrometer in the region between 400 and 4000 cm -1 .…”

Section: Analysis Of S-epsmentioning

confidence: 99%

Preparation of a sulfated exopolysaccharide (S-EPS) from Ophiocordyceps sinensis fungus and its antioxidant effects

Tran¹,

Nguyen²,

Nguyen³

et al. 2019

Sci. Tech. Dev. J. - Nat. Sci.

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Sulfated exopolysaccharides have been well-known to enhance biological activities. Exopolysaccharide (EPS) produced by Ophiocordyceps sinensis fungus is a source of natural compounds. The aim of our study is to improve the EPS biological activities by its sulfated modification using the chlorosulfonic acid (CSA)-pyridine (Pyr) method. The appropriate conditions of the sulfation reaction were explored, including CSA/Pyr ratio (v/v) of 1:3 and 6h. The degree of substitution (DS) of S-EPS11 was the highest (DS = 1.59). The total contents of polysaccharides and SO42- of S-EPS11 were 52.25% and 47.15%, respectively. Besides, the FT-IR spectra analysis indicated the presence of CO-S (peak of 815 cm-1) and S=O (peak of 1129 cm-1) stretching vibrations, while the natural EPS did not appear. Importantly, OH• and ABTS• radical scavenging potential of S-EPS11 significantly increased compared with those of the natural EPS. Together, we successfully generated sulfated EPS extracted from O. sinensis fungus which enhanced antioxidant activities of natural EPS.

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“…Among these four herbs, RSM shows diverse biological activities, such as inhibition of angiotensin converting enzyme (ACE), lowering blood pressure, dilate arteries, and decreasing blood clotting [18–20], thus is widely prescribed in different TCM formulae; RAM also shows protective effects against ultraviolet A-induced photoaging in human fibroblasts [21] and on proliferation and Akt phosphorylation of breast cancer cell lines [22]; isoflavones from RPL and their metabolites can inhibit growth and induce apoptosis in breast cancer cells [23]; ROJ plays a role in enhancing immunity, anti-myocardial ischemia, lowering blood glucose, and antiviral activity [24]. Impressively, RSM has shown synergisms with each of the other three herbs in clinical trials for cardiovascular disease (CVD) [17], the leading cause of morbidity and mortality all over the world [25].…”

Section: Introductionmentioning

confidence: 99%

A Systems Biology Approach to Uncovering Pharmacological Synergy in Herbal Medicines with Applications to Cardiovascular Disease

Wang

Xue

Tao

et al. 2012

Evidence-Based Complementary and Alternative Medicine

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Background. Clinical trials reveal that multiherb prescriptions of herbal medicine often exhibit pharmacological and therapeutic superiority in comparison to isolated single constituents. However, the synergistic mechanisms underlying this remain elusive. To address this question, a novel systems biology model integrating oral bioavailability and drug-likeness screening, target identification, and network pharmacology method has been constructed and applied to four clinically widely used herbs Radix Astragali Mongolici, Radix Puerariae Lobatae, Radix Ophiopogonis Japonici, and Radix Salviae Miltiorrhiza which exert synergistic effects of combined treatment of cardiovascular disease (CVD). Results. The results show that the structural properties of molecules in four herbs have substantial differences, and each herb can interact with significant target proteins related to CVD. Moreover, the bioactive ingredients from different herbs potentially act on the same molecular target (multiple-drug-one-target) and/or the functionally diverse targets but with potentially clinically relevant associations (multiple-drug-multiple-target-one-disease). From a molecular/systematic level, this explains why the herbs within a concoction could mutually enhance pharmacological synergy on a disease. Conclusions. The present work provides a new strategy not only for the understanding of pharmacological synergy in herbal medicine, but also for the rational discovery of potent drug/herb combinations that are individually subtherapeutic.

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The optimization of sulfation modification conditions for ophiopogonpolysaccharide based on antiviral activity

Cited by 31 publications

References 29 publications

Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide

Immune-enhancing activity comparison of sulfated ophiopogonpolysaccharide and sulfated jujube polysaccharide

Preparation of a sulfated exopolysaccharide (S-EPS) from Ophiocordyceps sinensis fungus and its antioxidant effects

A Systems Biology Approach to Uncovering Pharmacological Synergy in Herbal Medicines with Applications to Cardiovascular Disease

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