In vitro inhibition of mammary cell growth by lactoferrin: A comparative study

Hurley, W.L.; Hegarty, H.M.; Metzler, Jeremy

doi:10.1016/0024-3205(94)00528-1

Cited by 25 publications

(20 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, we found that lactoferrin inhibits the proliferation of these cells by an arrest in the cell cycle progression at the G1 to S transition. Our results are in agreement with previous data on the antiproliferative effect of lactoferrin on mammary cell lines [Rejman et al, 1992;Hurley et al, 1994]. This effect seems to be cell type specific as observed for other biomolecules such as growth factors and chemokines.…”

Section: Discussionsupporting

confidence: 93%

“…This effect remains however highly controversial. In fact, it has been reported that, in vitro, lactoferrin promotes the proliferation of the BALB/c 3T3 cell line [Azuma et al, 1989], has no effect on lymphocyte proliferation [Djeha and Brock, 1992], or inhibits mammary cell growth [Hurley et al, 1994;Damiens et al, 1998]. Furthermore, Campbell et al [1992] showed that lactoferrin may be downregulated in some forms of cancers such as human breast carcinoma suggesting that lactoferrin might regulate cell proliferation.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Lactoferrin inhibits G1 cyclin-dependent kinases during growth arrest of human breast carcinoma cells

et al. 1999

View full text Add to dashboard Cite

Lactoferrin inhibits cell proliferation and suppresses tumor growth in vivo. However, the molecular mechanisms underlying these effects remain unknown. In this in vitro study, we demonstrate that treatment of breast carcinoma cells MDA-MB-231 with human lactoferrin induces growth arrest at the G1 to S transition of the cell cycle. This G1 arrest is associated with a dramatic decrease in the protein levels of Cdk2 and cyclin E correlated with an inhibition of the Cdk2 kinase activity. Cdk4 activity is also significantly decreased in the treated cells and is accompanied by an increased expression of the Cdk inhibitor p21(CIP1). Furthermore, we show that lactoferrin maintains the cell cycle progression regulator retinoblastoma protein pRb in a hypophosphorylated form. Additional experiments with synchronized cells by serum depletion confirm the anti-proliferative activity of human lactoferrin. These effects of lactoferrin occur through a p53-independent mechanism both in MDA-MB-231 cells and other epithelial cell lines such as HBL-100, MCF-7, and HT-29. These findings demonstrate that lactoferrin induces growth arrest by modulating the expression and the activity of key G1 regulatory proteins.

show abstract

Section: Discussionsupporting

confidence: 93%

mentioning

confidence: 99%

Lactoferrin inhibits G1 cyclin-dependent kinases during growth arrest of human breast carcinoma cells

et al. 1999

View full text Add to dashboard Cite

show abstract

“…These results suggest that bLF has a possibility to present the other antitumor mechanisms like the induction of apoptosis except the leading of G1 arrest. The origin of LF causes different cell responses according to Hurley et al [5]. In our study, bLF had an antiproliferative effect on canine mammary tumor cells.…”

Section: Discussionsupporting

confidence: 63%

“…Hurley et al [5] reported that bLF and hLF had no effect on the growth of MCF-7 human breast tumor cells. On the other hand, Damien et al [2] reported that hLF inhibited the growth of MDA-MB 231 human breast cancer epithelial cells.…”

Section: Discussionmentioning

confidence: 99%

The Antiproliferative Effect of Bovine Lactoferrin on Canine Mammary Gland Tumor Cells

Yamada

Sato

Kobayashi

et al. 2008

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. Lactoferrin has several biological activities, including antitumor activities in some human and animal tumor cells. Clinical trials have been carried out in human medicine based on these effects. However, the antitumor effects of lactoferrin in veterinary medicine remain unknown. In this in vitro study, we demonstrated that co-incubation of canine mammary gland tumor cells (CIPp and CHMp) and bovine lactoferrin induced growth arrest of tumor cells. This growth arrest was associated with induction of G1 arrest. Furthermore, this effect was stronger in tumor cells than in normal cells. These findings demonstrate that bovine lactoferrin has anti-tumor activity in canine mammary tumors and has the potential for use in tumor-bearing dogs. KEY WORDS: bovine lactoferrin, canine mammary gland tumor cell, G1 arrest.

show abstract

“…Many other functions have been proposed for hLf including promotion of lymphocyte growth (13,14), immunostimulatory activity (15), production of cytokines (16), inhibition of mammary cell growth (17), activation Human lactoferrin (hLf) 2 is a member of the transof NK cells (18), and inhibition of platelet aggregation ferrin family and was originally found in milk (1). It (19).…”

Section: From Milk ᭧ 1998 Academic Pressmentioning

confidence: 99%