The Antiproliferative Effect of Bovine Lactoferrin on Canine Mammary Gland Tumor Cells

Abstract

ABSTRACT. Lactoferrin has several biological activities, including antitumor activities in some human and animal tumor cells. Clinical trials have been carried out in human medicine based on these effects. However, the antitumor effects of lactoferrin in veterinary medicine remain unknown. In this in vitro study, we demonstrated that co-incubation of canine mammary gland tumor cells (CIPp and CHMp) and bovine lactoferrin induced growth arrest of tumor cells. This growth arrest was associated with induction of G1 arrest. Furthermore, this effect was stronger in tumor cells than in normal cells. These findings demonstrate that bovine lactoferrin has anti-tumor activity in canine mammary tumors and has the potential for use in tumor-bearing dogs. KEY WORDS: bovine lactoferrin, canine mammary gland tumor cell, G1 arrest.

An Investigation of Growth Factors and Lactoferrin in Naturally Occurring Ovine Pulmonary Adenomatosis

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In vitro evaluation of bovine lactoferrin potential as an anticancer agent

“…Due to safety concerns on the use of pharmaceuticals, as well as their regulatory limitations, dietary agents are becoming attractive alternatives as cancer chemo-preventive agents (Korhonen & Pihlanto, 2006;Wakabayashi, Yamauchi, & Takase, 2006). Some dietary agents, such as selenium (Yamada et al, 2008), vitamin D and green tea polyphenols have been described as interesting chemo-preventive agents for prostate cancer (Syed, Khan, Afaq, & Mukhtar, 2007).…”

Bovine lactoferrin inhibits lung cancer growth through suppression of both inflammation and expression of vascular endothelial growth factor

“…Bovine lactoferrin (bLF), a siderophilic protein with 2 iron-binding sites, has a wide range of biological activities, including anticancer effects, antimicrobial effects, and improvement of immunomodulatory functions (de Mejia and Dia, 2010). Chemopreventive and cell growth inhibitory activities of bLF have been demonstrated in esophageal (Ushida et al, 1999), lung (Li et al, 2011), colon (Tsuda et al, 1998), bladder (Masuda et al, 2000), mammary (Yamada et al, 2008;Duarte et al, 2011), stomach (Xu et al, 2010), and tongue (Tanaka et al, 2000) cancers. The anticancer functions of bLF are thought to be exerted through its innate…”