In Vitro Inhibition of Oral Candida albicans by Chicken Egg Yolk Antibody (IgY)

Abstract

This study was conducted to measure Candida albicans-specific chicken egg yolk antibody (IgY) inhibition of fluconazole-sensitive and resistant strains of C. albicans in order to assess potential use in the prevention and treatment of oral candidiasis. In this study, laying hens were immunized, and IgY was extracted by water dilution. The Minimal Inhibitory Concentrations (MICs) of IgY for inhibiting C. albicans growth were determined using the broth microdilution method from the CLSI M27-A2 protocol. Fluconazole (FLC) was used as the control. The results were analyzed with the chi(2) test. The anti-Candida titer of anti-C. albicans IgY was 1:12,000. The concentration of the IgY extract that effectively inhibited the growth of C. albicans was between 1.25 g/l and 5.0 g/l, and the efficacy rate was 82.98% during the observed 24-48 h time period. No correlation was recorded between the drug resistance of FLC and growth inhibition by IgY. It was concluded that anti-C. albicans IgY inhibited the growth of C. albicans in vitro and there was no correlation between the drug resistance of FLC and the growth inhibition by IgY (P > 0.99).

Dengue virus specific IgY provides protection following lethal dengue virus challenge and is neutralizing in the absence of inducing antibody dependent enhancement

“…The therapeutic potential of polyclonal avian-derived IgY has been previously established for the treatment of Pseudomonas aeruginosa , which is in an ongoing phase III clinical trial under the auspices of the European Medicines Agencies [58–60], in addition to treatment of Candida albicans , [61, 62], and many toxins and venoms [63–65]. Although the animal models used in our study and others demonstrate the therapeutic efficacy of IgY, we recognize that there are potential obstacles that may arise in translating IgY therapeutics for human clinical application.…”

Use of Candida‐specific chicken egg yolk antibodies to inhibit the adhering of Candida to denture base materials: prevention of denture stomatitis

“…If an effective preventive measure using IgYs against Candida is established, it will be possible to reduce the dosage of concomitant antifungal drugs and thus to reduce the emergence of resistant strains. Regarding this, Wang et al . reported that anti‐C.a.IgY inhibited the growth of C. albicans in vitro, and there was no correlation between drug resistance to fluconazole and the growth inhibition by IgY.…”

Production of anti-Trichophyton rubrum egg yolk immunoglobulin and its therapeutic potential for treating dermatophytosis

“…There were also other studies about antifungal IgYs such as anti-C. albicans IgYs [14,26,27]. One of the studies evaluated in vitro and in vivo effectiveness of IgY against C. albicans by using proteins from sonicated cells of C. albicans as antigen to immunized hens.…”

“…Effective protection against Salmonella enteritidis, Salmonella e. Typhimurium, Campylobacter jejuni, Escherichia coli ETEC, murine and bovine rotavirus, and bovine coronavirus infections in mice, pig, and calves has been obtained with the use of passively-administered egg yolk-derived antibodies [12]. Ample data have demonstrated the protection of IgY against different kinds of bacteria, virus, and parasites There are also reports about anti-fungi IgYs [13,14], reminding us that it may also be effective against T. rubrum.…”