In-Vitro Inhibition of Staphylococcal Pathogenesis by Witch-Hazel and Green Tea Extracts

Rasooly, Reuven; Molnar, Adel; Choi, Hwang-Yong; Paula, María; Racicot, Kenneth; Apostolidis, Emmanouil

doi:10.3390/antibiotics8040244

Cited by 19 publications

(21 citation statements)

References 37 publications

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“…Therefore, to enhance the antibacterial activity of iodine, we added witch hazel extract that contains high levels of hamamelitannin (HAMA), because of its known anti-biofilm properties [14]. The witch hazel extract used (whISOBAX, StaphOff Biotech Inc) (WH) has 50 mg/mL total dry weight (35% of that is due to HAMA), and a total phenolic content of 12.66 mg/mL GAE (76% of that is due to HAMA) [31]. DIP and WH were tested for their antibacterial activity.…”

Section: Resultsmentioning

confidence: 99%

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Witch Hazel Significantly Improves the Efficacy of Commercially Available Teat Dips

et al. 2020

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Bovine intramammary infections (IMIs) are the main cause of economic loss in milk production. Antibiotics are often ineffective in treating infections due to antimicrobial resistance and the formation of bacterial biofilms that enhance bacterial survival and persistence. Teat dips containing germicides are recommended to prevent new IMIs and improve udder health and milk quality. IMIs are often caused by staphylococci, which are Gram-positive bacteria that become pathogenic by forming biofilms and producing toxins. As a model for a teat dip (DIP), the BacStop iodine-based teat dip (DIP) was used. Witch hazel extract (whISOBAX (WH)) was tested because it contains a high concentration of the anti-biofilm/anti-toxin phenolic compound hamamelitannin. We found that the minimal inhibitory or bactericidal concentrations of DIP against planktonic S. epidermidis cells increased up to 160-fold in the presence of WH, and that DIP was 10-fold less effective against biofilm cells. While both DIP and WH are effective in inhibiting the growth of S. aureus, only WH inhibits toxin production (tested for enterotoxin-A). Importantly, WH also significantly enhances the antibacterial effect of DIP against Gram-negative bacteria that can cause IMIs, like Escherichia coli and Pseudomonas aeruginosa. Put together, these results suggest that the antibacterial activity of DIP combined with WH is significantly higher, and thus have potential in eradicating bacterial infections, both in acute (planktonic-associated) and in chronic (biofilm-associated) conditions.

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Section: Resultsmentioning

confidence: 99%

“…Witch hazel extract (whISOBAX, 50 mg/mL StaphOff Biotech Inc, MA, USA) containing 50 mg/mL dry weight. Its phenolic content is 12.66 mg/mL gallic-acid equivalent (GAE), where 76% of that is due to hamamelitannin [31]. Dilution factors used in the experiments described are shown in Tables 4 and 5.…”

Section: Test Formulationsmentioning

confidence: 99%

Witch Hazel Significantly Improves the Efficacy of Commercially Available Teat Dips

et al. 2020

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“…Among them, the enterotoxin A is responsible for most staphylococcal food poisoning outbreaks [141]. Phenolic compounds can affect the enterotoxin production through several mode of action, including translation and/or transcription inhibition, disruption of secretory mechanisms, inhibition of quorum sensing regulatory systems, and toxin inactivation [142,143]. Various plant derived phenolic substances have been described as effective inhibitors of the staphylococcal enterotoxin production and activity (Table 5).…”

Section: Anti-enterotoxin Effect Of Plant Phenolicsmentioning

confidence: 99%

Plant Phenolics and Phenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms

et al. 2020

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Phenolic compounds and extracts with bioactive properties can be obtained from many kinds of plant materials. These natural substances have gained attention in the food research as possible growth inhibitors of foodborne pathogenic and spoilage bacteria. Many phenolic-enriched plant extracts and individual phenolics have promising anti-quorum sensing potential as well and can suppress the biofilm formation and toxin production of food-related pathogens. Various studies have shown that plant phenolics can substitute or support the activity of synthetic food preservatives and disinfectants, which, by the way, can provoke serious concerns in consumers. In this review, we will provide a brief insight into the bioactive properties, i.e., the antimicrobial, anti-quorum sensing, anti-biofilm and anti-enterotoxin activities, of plant phenolic extracts and compounds, with special attention to pathogen microorganisms that have food relation. Carbohydrase aided applications to improve the antimicrobial properties of phenolic extracts are also discussed.

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“…Based on these findings, we conclude that there is insufficient evidence to support the efficacy and the recommendation of CAM for acne, AD, and psoriasis. Conversely, our systematic Coconut oil [47][48][49][50][51] Aloe vera [52][53][54][55] Honey 7,56,57 Turmeric 23,[48][49][50][51][52][53][54][55][56][57][58][59][60][61][62] Colloidal oatmeal 63,64 Sunflower seed 51,[65][66][67][68][69][70][71] Shea butter [72][73][74][75] Witch hazel [76][77][78] Tea tree oil [79][80][81][82]…”

Section: Resultsmentioning

confidence: 99%

Complementary and alternative medicine treatments for common skin diseases: A systematic review and meta-analysis

et al. 2021

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Background Complementary and alternative medicine (CAM) treatments are growing in popularity as alternative treatments for common skin conditions. Objectives To perform a systematic review and meta-analysis to determine the tolerability and treatment response to CAM treatments in acne, atopic dermatitis (AD), and psoriasis. Methods PubMed/Medline and Embase databases were searched to identify eligible studies measuring the effects of CAM in acne, AD, and psoriasis. Effect size with 95% confidence interval (CI) was estimated using the random-effect model. Results The search yielded 417 articles; 40 studies met the inclusion criteria. The quantitative results of CAM treatment showed a standard mean difference (SMD) of 3.78 (95% CI [−0.01, 7.57]) and 0.58 (95% CI [−6.99, 8.15]) in the acne total lesion count, a SMD of −0.70 (95% CI [−1.19, −0.21]) in the eczema area and severity index score and a SMD of 0.94 (95% CI [−0.83, 2.71]) in the scoring of atopic dermatitis score for AD, and a SMD of 3.04 (95% CI [−0.35, 6.43]) and 5.16 (95% CI [−0.52, 10.85]) in the Psoriasis Area Severity Index score for psoriasis. Limitations Differences between the study designs, sample sizes, outcome measures, and treatment durations limit the generalizability of data. Conclusions Based on our quantitative findings we conclude that there is insufficient evidence to support the efficacy and the recommendation of CAM for acne, AD, and psoriasis.

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In-Vitro Inhibition of Staphylococcal Pathogenesis by Witch-Hazel and Green Tea Extracts

Cited by 19 publications

References 37 publications

Witch Hazel Significantly Improves the Efficacy of Commercially Available Teat Dips

Witch Hazel Significantly Improves the Efficacy of Commercially Available Teat Dips

Plant Phenolics and Phenolic-Enriched Extracts as Antimicrobial Agents against Food-Contaminating Microorganisms

Complementary and alternative medicine treatments for common skin diseases: A systematic review and meta-analysis

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