In vitro inhibitory effect of aflatoxin B1 on acetylcholinesterase activity in mouse brain

Cometa, Maria Francesca; Lorenzini, Paola; Fortuna, Stefano; Volpe, Maria Teresa; Meneguz, Annarita; Palmery, Maura

doi:10.1016/j.tox.2004.07.009

Cited by 38 publications

(23 citation statements)

References 39 publications

(53 reference statements)

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“…The first work that has reported the ability to inhibit AChE enzyme was published on Toxicology by Cometa et al [203]. In this work the inhibition of AChE extracted from mouse brain by AFB 1 , was studied obtaining IC 50 equal 10 ppm.…”

Section: Effect Of Enzyme Sources Incubation Time and Enzyme Loadingmentioning

confidence: 98%

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

et al. 2010

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The present review reports the research carried out during last 9 years on biosensors based on cholinesterase inhibition for nerve agents, organophosphorus and carbammic insecticides, and aflatoxin B 1 detection. Relative applications in environmental and food areas are also reported. Special attention is paid to the optimization of parameters such as enzyme immobilization, substrate concentration, and incubation time in the case of reversible inhibition by aflatoxin B 1 or irreversible inhibition by organophosphorus and carbamic insecticides, and nerve agents in order to optimize and improve the analytical performances of the biosensor. Evaluation of selectivity of the system is also discussed.

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Section: Effect Of Enzyme Sources Incubation Time and Enzyme Loadingmentioning

confidence: 98%

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

et al. 2010

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“…In the case of the aflatoxins, this approach pointed to either an enzyme that metabolizes AFB 1 into a detectable compound or an enzyme that can be inhibited by AFB 1 . The inhibition by AFB 1 of acetylcholinesterase (AChE), a key enzyme in the transmission of nerve impulses has been investigated (Cometa et al, 2005). The AFB 1 inhibition towards AChE extracted from mouse brain was studied and the implications in terms of kinetic mechanism and toxicity discussed.…”

Section: Introductionmentioning

confidence: 99%

Development of a bio-electrochemical assay for AFB1 detection in olive oil

Rejeb

Arduini

Arvinte

et al. 2009

Biosensors and Bioelectronics

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“…Animal studies demonstrated that the ventral mesencephalon, hippocampus, striatum, and cerebellum are the main OTA targets in the brain of adult rats exposed to OTA, excluding the rest of the brain (Belmadani et al 1998). Other proposed mechanisms are: AFB1 non-competitively inhibits mouse brain AChE by blocking access of the substrate to the active site or by inducing a defective conformational change in the enzyme through non-covalent binding interacting with the AChE peripheral binding site (Cometa et al 2005).…”

Section: Discussionmentioning

confidence: 99%

Toxicological Studies of Mycotoxins Using Enzymatic and Histochemical Methods

Badea

Taus

Potroviță³

et al. 2009

Water Air Soil Pollut: Focus

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Studies concerning mycotoxins involve activities of relevant potential for furthering knowledge in the fields of toxicology and environmental analysis. Using bioanalytical methods (biosensors, histochemistry), the conducted research aims at contributing to raising the awareness of local, national, and international media in relation to the safety of obtaining and processing vegetal and animal foods, by analyzing the possible effects of aflatoxins and ochratoxins, promoting animal health, food hygiene, in view of ensuring animal and human health. The study using laboratory animals (mice) while being part of one of the current national research directions, also holds international priority, by its contribution to a better understanding of several fundamental mechanisms of life at molecular level and to the characterization of certain biological processes that appear in mycotoxicosis.

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In vitro inhibitory effect of aflatoxin B1 on acetylcholinesterase activity in mouse brain

Cited by 38 publications

References 39 publications

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

Biosensors based on cholinesterase inhibition for insecticides, nerve agents and aflatoxin B1 detection (review)

Development of a bio-electrochemical assay for AFB1 detection in olive oil

Toxicological Studies of Mycotoxins Using Enzymatic and Histochemical Methods

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