In Vitro Inhibitory Effects of Viburnum opulus Bark and Flower Extracts on Digestion of Potato Starch and Carbohydrate Hydrolases Activity

Abstract

One of the effective treatments for diabetes is to reduce and delay the absorption of glucose by inhibition of α-amylase and α-glucosidase in the digestive tract. Currently, there is a great interest in natural inhibitors from various part of plants. In the present study, the phenolic compounds composition of V. opulus bark and flower, and their inhibitory effects on in vitro potato starch digestion as well as on α-amylase and α-glucosidase, have been studied. Bark and flower phenolic extracts reduced the amount of glucose released from potato starch during tree-stage simulated digestion, with IC50 value equal to 87.77 µg/mL and 148.87 µg/mL, respectively. Phenolic bark extract showed 34.9% and 38.4% more potent inhibitory activity against α-amylase and α-glucosidase, respectively, but the activity of plant extracts was lower than that of acarbose. Chlorogenic acid (27.26% of total phenolics) and (+)-catechin (30.48% of total phenolics) were the most prominent phenolics in the flower and bark extracts, respectively. Procyanidins may be responsible for the strongest V. opulus bark inhibitory activity against α-amylase, while (+)-catechin relative to α-glucosidase. This preliminary study provides the basis of further examination of the suitability of V. opulus bark compounds as components of nutraceuticals and functional foods with antidiabetic activity.

Histological and Physiological Study of the Effects of Biostimulants and Plant Growth Stimulants in Viburnum opulus ‘Roseum’

“…There are several ornamental plant species that have been very popular and widely used since their first commercialisation, but their tolerance needs to be supported at several levels as environmental conditions change. The traditional species V. opulus ‘Roseum’ is a conspicuous ornamental shrub, both in its habit and inflorescence, as described by Kajszczak et al [ 50 ]. The species can currently be grown safely in urban conditions, but in a few years its cultivation may become endangered and as it is less resistant to the changing urban environment.…”

“…Each flower is composed of an outer ring of large sterile flowers and an inner ring of small fertile flowers. The ornamental cultivar ‘Roseum’ has only sterile flower spikes, which give it a snowball-like appearance [ 50 ].…”

LED Light Quality Affected Bioactive Compounds, Antioxidant Potential, and Nutritional Value of Red and White Cabbage Microgreens

“…The qualitative and quantitative composition of phenolic compounds was estimated using an Acquity ultra-performance liquid chromatography (UPLC) system that was cou-pled with a quadruple-time of flight mass spectrometry (Q-TOF-MS) instrument (Waters Corp., Milford, MA, USA) equipped with an electrospray ionization (ESI) source. The conditions of UPLC-PDA and MS/MS were described in our previous study [27]. Chromatographic separation was performed using an Acquity HSS T3 C18 column (150 mm × 2.1 mm, 1.8 µm; Waters, Milford, MA, USA) at 30 • C with a flow rate of 0.45 mL min −1 , and an injection volume of 5 µL.…”

In vitro gastrointestinal digestion methods of carbohydrate‐rich foods

“…RSIE has been used in the study of α‐glucosidase inhibition, with the purpose of decreasing the hydrolysis and excessive absorption of carbohydrates in the small intestine (Ohta et al., 2002 ; Oki et al., 1999 ; Shinde et al., 2008 ; Zhang et al., 2011 ). Nevertheless, the current methodology shows that there are several drawbacks, mainly regarding enzymatic activity (Ferreira‐Lazarte, Montilla, et al., 2017 ; Ferreira‐Lazarte, Olano, et al., 2017 ; Gallego‐Lobillo et al., 2021 ; Kajszczak et al., 2022 ; Lee et al., 2016 ; Seo et al., 2020 ; Shin et al., 2019 ). The hydrolysis temperature (37°C) has been the only parameter in common between different reports.…”