In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes

Olasehinde, Oluwaseun Ruth; Afolabi, Olakunle Bamikole; Omiyale, Benjamin Olusola; Olaoye, Oyindamola A.

doi:10.34172/jhp.2021.48

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…For solvent extractions, 3 gms of the test powder was mixed with 20 ml of 70% methanol 18 and 70% ethanol 19 separately. In RT, this mixture was kept for a period of 3 days with occasional shaking to facilitate the extraction process.…”

Section: Plant Extracts Preparationmentioning

confidence: 99%

Role of flower extract in preventing hyperlipidemia and liver lipid peroxidation in male mice

Kar,

J Jha,

Mahar

2024

IJPP

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: Hyperlipidemia leads to coronary artery disease. Although statins are used conventionally, they are often associated with side effects. It has now been attempted to study the role of flower (MOF) extract in the regulation of tyloxapol-induced hyperlipidemia and tissue lipid peroxidation (LPO) in male mice.Six groups of animals were taken. Group I acted as control and group II, receiving tyloxapol (300 mg/kg, single dose on 14 day) served as hyperlipidemic control. Group III received simvastatin (200 mg/kg), while group IV, V and VI received MOF extract at 400, 200 and 100 mg/kg every day respectively for 15 days. These four groups (III- VI) also received same amount of tyloxapol on 14 day. On day 16 changes in the serum total cholesterol, triglycerides, high-density lipoprotein, low-density lipoprotein and very low-density lipoprotein; hepatic LPO, super oxide dismutase, catalase, glutathione peroxidase and histological changes in liver were analyzed. Total phenolic and flavonoid contents were also estimated. The in-vitro antioxidative property was checked through DPPH and HO assays.: Results showed significant reduction in all the serum lipids except HDL, which was increased in MOF treated hyperlipidemic mice, with the parallel decrease in hepatic LPO and increase in antioxidants. Histological studies also showed reduction in hepatic damage with the pre-treatment of MOF. However, the most effective dose was found to be 400 mg/kg of MOF. : We suggest that flower extract may ameliorate hyperlipidemia with antioxidative benefits.

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Section: Plant Extracts Preparationmentioning

confidence: 99%

Role of flower extract in preventing hyperlipidemia and liver lipid peroxidation in male mice

Kar,

J Jha,

Mahar

2024

IJPP

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“…Numerous experimental findings have provided evidence for the significance of reactive oxygen species (ROS) participation in the pathophysiology of DM, particularly in the development of complications associated with the condition (3). The production of ROS has been observed in β-cell dysfunction and death in both types of DM.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant capacities, antidiabetic potentials, and mineral compositions of pap aqua and aqueous extracts from Ocimum gratissimum L.

Agunbiade,

Afolabi,

Jaiyesimi

et al. 2024

J Herbmed Pharmacol

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Introduction: This study appraised the antioxidant potentials, mineral compositions, and antidiabetic activities of pap water (aqua) extract (PWE) and aqueous extract (AE) from Ocimum gratissimum. Methods: The total phenolic contents (TPC), total flavonoid contents (TFC), ferric reducing antioxidant powers (FRAP), Fe2+-chelating abilities, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging abilities, and enzymes inhibition potentials of the PWE and AE from O. gratissimum were evaluated. Additionally, mineral contents were determined using absorptive absorbance spectroscopy (AAS). Results: The PWE of O. gratissimum exhibited higher TPC of 42.61± 0.04 mg gallic acid equivalent per gram of dried sample (GAE/g) and TFC of 85.7 ± 0.02 µg quercetin equivalent per gram of dried sample (QE/g). In contrast, AE had lower TPC (21.52 ± 0.01 mg GAE/g) and TFC (55.0 ± 0.01 µg QE/g). PWE also displayed a lower FRAP of 2.86 ± 0.01 mg AAE/g, while AE had a higher FRAP of 2.94 ± 0.03 mg AAE/g. PWE of O. gratissimum had IC50 for DPPH: 100.00 µg/mL, Fe2+-chelating ability: 4.41 µg/mL, while AE had IC50 for DPPH: 140.00 µg/mL and Fe2+-chelating ability: 4.90 µg/mL. Similarly, the PWE of O. gratissimum showed a higher α-amylase inhibition (IC50: 0.47 mg/mL) than AE (IC50: 0.78 mg/mL); however, AE (IC50 =3.09 µg/mL) demonstrated a higher α-glucosidase inhibition than PWE (IC50: 9.09 µg/mL). AAS analyses indicated the presence of Ca, Fe, Mg, Cu, Zn, and Mn in different proportions in both extracts. Conclusion: Therefore, PWE could be a better alternative in the management of diabetes melitus if properly annexed.

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“…Oxidative stress, defined as an imbalance between reactive oxygen species (ROS)/electrophiles (free radicals) and the antioxidant system, is known to disrupt cellular redox signaling and thus aggravate the severity of a variety of pathological conditions (14). However, evidence suggests that elevated systemic oxidative stress is closely linked to the etiology of diabetes (15).…”

Section: Introductionmentioning

confidence: 99%

Identification of bioactive constituents of chloroform fraction from Annona muricata leaf, its antioxidant activity and inhibitory potential against carbohydrate-hydrolyzing α-amylase and α-glucosidase activities linked to type II diabetes mellitus: In vitro study

Olasehinde

Afolabi

2022

J Herbmed Pharmacol

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Introduction: Secondary metabolites from plants have been found to play an important role in the treatment of diabetes mellitus (DM) and its complications. Therefore, the purpose of this study was to identify the chemical components of Annona muricata leaf chloroform fraction (CFAm) and its in vitro antioxidant properties, as well as inhibitory activity against α-amylase and α-glucosidase enzymatic activities. Methods: Gas chromatography–mass spectrometry (GC-MS) technique was engaged in the identification of phytochemical constituents. Antioxidant activities such as DPPH free radical scavenging ability, reducing power capacity, hydroxyl radical scavenging ability, singlet oxygen scavenging capacity, as well as α-amylase and α-glucosidase inhibition were carried out using standard in vitro methods. Results: GC-MS analysis of CFAm revealed the presence of 23 phytochemicals, out of which 5 compounds had the highest % compositions (i.e., octadecanoic acid (20.35%), 2-propanone, 1-(4-hydroxy-3-methoxyphenyl) (12.04%), isocomene (22.60%), 9, 12, 15 octadecatrienoic acid, methyl ester (Z,Z,Z) (28. 98%), and quercetin, 5TMS derivative (18.28%)). Also, CFAm demonstrated a significant (P<0.05) inhibition against DPPH (IC50= 26.33±1.39 mg/mL), with OH free radical scavenging capacity (65.46 ± 1.39 mg/100 g), singlet oxygen scavenging capacity (55.24 ± 1.22 mg/100 g), and showed ferric reducing power (84.52 ± 2.84 mg/100 g). Also, CFAm exhibited a significant (P<0.05) inhibition against α-glucosidase (IC50= 71.06 ±1.45 mg/ mL) and α-amylase (IC50= 73.88±1.58 mg/mL) in a concentration-dependent manners. Conclusion: The remarkable properties demonstrated by CFAm, which are essential for the management of DM, could probably be credited to the presence of the various identified phytonutrients.

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In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes

Cited by 4 publications

References 35 publications

Role of flower extract in preventing hyperlipidemia and liver lipid peroxidation in male mice

Role of flower extract in preventing hyperlipidemia and liver lipid peroxidation in male mice

Antioxidant capacities, antidiabetic potentials, and mineral compositions of pap aqua and aqueous extracts from Ocimum gratissimum L.

Identification of bioactive constituents of chloroform fraction from Annona muricata leaf, its antioxidant activity and inhibitory potential against carbohydrate-hydrolyzing α-amylase and α-glucosidase activities linked to type II diabetes mellitus: In vitro study

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