In vitro interactions between farnesol and fluconazole, amphotericin B or micafungin against Candida albicans biofilms

Abstract

Farnesol exerts a synergistic or additive interaction with micafungin, fluconazole and amphotericin B against C. albicans biofilms, thus warranting further in vivo study.

Effect of caspofungin and micafungin in combination with farnesol against Candida parapsilosis biofilms

“…The working concentrations for drug were prepared in RPMI-1640. Each drug-free control well contained 1% (vol/vol) methanol [16].…”

“…with the antifungal free control [16]. Percent of change of turbidity was calculated on the basis of absorbance (A) as 100% × (A well -A background )/(A drug-free well -A background ).…”

“…Percent of change of turbidity was calculated on the basis of absorbance (A) as 100% × (A well -A background )/(A drug-free well -A background ). The background was measured from the fungus-free well [16,17].…”

“…Metabolic activity was quantified by XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) assay, as previously described [16,17]. XTT solution (0.5 g/L)…”

“…Drug interactions were measured by a two-dimensional broth microdilution checkerboard assay both for planktonic and sessile cells [16,18,19]. Based on our preliminary results the examined concentration ranges were the same as mentioned above at planktonic and sessile To analyse drug-drug interactions further, we built Bliss independence models (BIs) using MacSynergy II software [20].…”