In vitro lead cation-hyaluronic add interaction

Villegas-Navarro, A.; A, Rocio Dominguez; Reyes, José Luis; A, Teófilo A. Dieck

doi:10.1016/s0940-2993(11)80359-3

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…Purchased as frozen liquid and has been filtered through a 0.1 mm pore filter 5. Artificial synovial fluid 8 7.4 Hyaluronic acid from humam umbilical cord (3 g) and plasma proteins (1.5 g) in 1 liter Tris-NaCl 0.05 M 6. Artificial gastric juice (ASTM D5517)…”

Section: Methodsmentioning

confidence: 99%

Bioaccessibility testing of cobalt compounds

Stopford

Turner

Cappellini

et al. 2003

J. Environ. Monitor.

192

155

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Testing of metal compounds for solubility in artificial fluids has been used for many years to assist determining human health risk from exposure to specific compounds of concern. In lieu of obtaining bioavailability data from samples of urine, blood, or other tissues, these studies measured solubility of compounds in various artificial fluids as a surrogate for bioavailability. In this context, the measurement of metal "bioaccessibility" can be used as an in vitro substitute for measuring metal bioavailability. Bioaccessibility can be defined as a value representing the availability of metal for absorption when dissolved in in vitro surrogates of body fluids or juices. The aim of this study was to measure and compare the bioaccessibility of selected cobalt compounds in artificial human tissue fluids and human serum. A second aim was to initiate studies to experimentally validate an in vitro methodology that would provide a conservative estimate of cobalt bioavailability in the assessment of dose from human exposure to various species of cobalt compounds. This study evaluated the bioaccessibility of cobalt(II) from 11 selected cobalt compounds and an alloy in 2 physical forms in 5 surrogate human tissue fluids and human serum. Four (4) separate extraction times were used up to 72 hours. The effect of variables such as pH, dissolution time, and mass-ion effect on cobalt bioaccessibility were assessed as well. We found that the species of cobalt compound as well as the physico-chemical properties of the surrogate fluids, especially pH, had a major impact on cobalt solubility. Cobalt salts such as cobalt(II) sulfate heptahydrate were highly soluble, whereas cobalt alloys used in medical implants and cobalt aluminate spinels used as pigments, showed minimal dissolution over the period of the assay.

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Section: Methodsmentioning

confidence: 99%

Bioaccessibility testing of cobalt compounds

Stopford

Turner

Cappellini

et al. 2003

J. Environ. Monitor.

192

155

View full text Add to dashboard Cite

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“…33 Collectively, these studies raise the possibility that oral HA could indirectly affect osteopenia by binding to HA receptors such as TLR-4 on immune cells within the gut epithelium, resulting in altered systemic inflammatory cytokine production, which attenuates osteoclastogenesis to reduce susceptibility to osteopenia. One alternative mechanisms is that HA, which efficiently binds to cations 34 including calcium, 35,36 may promote gut uptake and increase serum levels of this cation, which has been related to bone density. Analysis of rat models of calcium insufficiency might be useful in assessing this last possibility.…”

Section: ■ Discussionmentioning

confidence: 99%

Oral Administration of Hyaluronan Reduces Bone Turnover in Ovariectomized Rats

Granton

Holdsworth

et al. 2013

J. Agric. Food Chem.

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The effect of oral hyaluronan (HA) on bone loss in ovariectomized (OVX) 3-month-old rats was measured using serum markers of bone turnover and bone mineral density. OVX rats were administered 1 mg/kg HA (OVX + HA) or phosphate-buffered saline (PBS) (OVX + PBS) by oral gavage (5 days/week for 54 days). Additional controls included sham ovariectomy with PBS gavage (Sham + PBS) and no treatment. Oral administration of HA resulted in approximately 50% (p < 0.05) increases in serum HA. Gel filtration analyses showed this was high molecular weight HA (300-500 kDa). Osteopenia was mild due to the young age of the animals. Thus, ovariectomy resulted in a 30% increase in serum collagen N-terminal telopeptides (p < 0.001), a 20% increase in serum nitrate/nitrite levels (p = 0.05), and a 5-6% decrease in femur bone mineral density/content (p < 0.05). HA gavage blunted the development of osteopenia in this model as determined by preventing the 30% increase in serum collagen N-terminal telopeptide levels (p < 0.001) and by reducing bone mineral content loss from 6 to 4%. These results show that oral supplements of HA (gavage solution, 0.12% solution) significantly reduce bone turnover associated with mild osteopenia in rats.

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