2016
DOI: 10.1016/j.jarmap.2015.12.001
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In vitro mass propagation of Cannabis sativa L.: A protocol refinement using novel aromatic cytokinin meta-topolin and the assessment of eco-physiological, biochemical and genetic fidelity of micropropagated plants

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Cited by 82 publications
(99 citation statements)
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References 42 publications
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“…The present investigation suggests that the IAA showed the least response in root formation. In comparison with other auxins, a similar observation was reported 28 . Tissue culture techniques can play an important role in clonal propagation of elite genotypes of has diverse medicinal applications and eventually due to overexploitation and irregular concern this plant is facing local extinction.…”
Section: Methodssupporting
confidence: 82%
“…The present investigation suggests that the IAA showed the least response in root formation. In comparison with other auxins, a similar observation was reported 28 . Tissue culture techniques can play an important role in clonal propagation of elite genotypes of has diverse medicinal applications and eventually due to overexploitation and irregular concern this plant is facing local extinction.…”
Section: Methodssupporting
confidence: 82%
“…Given the obligate outcrossing nature of Cannabis [34] as well as heterozygosity within the gene pool [3, 35], seed-grown Cannabis botanical raw material can be highly variable in content and composition of target and non-target active ingredients [31, 36]. Thus, clonal propagation using callus- [37] or nodal- (axillary buds) [38] mediated regeneration is favoured over seed propagation [22], with explants typically grown under environmentally controlled conditions in an effort to minimise genetic by environment interactions and to maximise product uniformity [31]. …”
Section: Cannabis Drug Formsmentioning
confidence: 99%
“…However, the efficiency of completely regenerated plants was very low [14]. With the use of the cytokinin meta-topolin, the group of ElSohly [17] was successful. They were able to establish a one-step protocol for plant regeneration out of axillary bud explants with direct organogenesis.…”
Section: Transgenic Cannabis Plantsmentioning
confidence: 99%