In vitro Mass Propagation of Cymbidium aloifolium (L.) Sw.

Abstract

Cymbidium aloifolium (L.) Sw. is an epiphytic orchid distributed locally in Bangladesh. Natural populations of this orchid are under threat due to its high commercial value in the floriculture industry by over exploitation. Mass propagation provides an alternative means of satisfying the demand. In this study, half strength of MS supplemented with BAP or different combinations with NAA was evaluated for seed germination and early protocorm development of C. aloifolium (L.) Sw. The maximum percentage of seed germination (98) was obtained on modified MS (half strength of micro and macro nutrients and full strength of vitamin) supplemented with 0.5 mg/l BAP and 0.5 mg/l NAA after three weeks of culture. Maximum number of shoots (9.6 shoots per single shoot) were developed from shoot tip explants on half strength of MS supplemented with 1.0 mg/l BAP and 0.5 mg/l NAA. The most effective auxin source promoting root production (3.2 roots per shoot) was 1.0 mg/l IBA in combination with 0.5 mg/l BAP. The seedlings were successfully acclimatized (survival percentage 99) in a nethouse using a rooting medium of crushed sterile brick, cocopit and charcoal (1 : 1 : 1 v/v).
 Plant Tissue Cult. & Biotech. 29(1): 73-79, 2019 (June)

Effects of Different Natural Extracts and Plant Growth Regulators on Plant Regeneration and Callus Induction from Pseudobulbs Explants through in vitro Seed Germination of Endangered Orchid Bulbophyllum auricomum Lindl.

“…and can produce many micropropagules in a short period of time that have almost the same properties as the parent and can save these endangered species from extinction in their natural habitat. Furthermore, epiphytic orchids have been developed and are being successfully cultured in MS (Murashige and Skoog) media (Paul et al, 2019). Activated charcoal (AC), plant growth regulators (PGRs), and organic growth supplements have also been shown to influence in vitro shoot regeneration, multiplication, and growth of orchid seedlings (Kim et al, 2019).…”

Desarrollo in vitro de la orquídea epífita Erycina hyalinobulbon (Orchidaceae), endémica de México, para promover su conservación

“…These plants can be offered on the market to reduce the extraction pressure of individuals from wild populations (Ávila-Díaz et al, 2009;Menchaca et al, 2012). Diverse in vitro propagation protocols of epiphytic orchids have been developed, being successful the uses of Murashige & Skoog culture media (Murashige and Skoog, 1962) (MS) (Ávila-Díaz et al, 2009;Paul et al, 2019), Phytamax™ (Ruíz et al, 2008;Pérez-Decelis et al, 2016), and Knudson C (Acemi and Özen, 2019;Pyati, 2019), among others, whether they are supplemented or not, and with plant growth promoters. The use of organic components can be a possible substitute for synthetic phytoregulators, lowering the costs to propagate orchids in vitro, as was the case of the MS medium supplemented with coconut water, which presented greater germination efficiency and the formation of seedlings in a Phalaenopsis hybrid (Salazar et al, 2013).…”

Induction of Fingerroot (Boesenbergia Pandurata Roxb.) Buds in Various MS Media and Sucrose

“…Tissue culture techniques have been widely exploited for large-scale propagation. Plant micropropagation through tissue culture has become a significant and informative technique for producing and maintaining plant availability [6]. In vitro cultivars from many plants can accumulate various groups of plant metabolites with antioxidant activity including flavonoids [7] so that the content of panduratin A in fingerroot can be increased.…”

“…In vitro cultivars from many plants can accumulate various groups of plant metabolites with antioxidant activity including flavonoids [7] so that the content of panduratin A in fingerroot can be increased. Plants produced through micropropagation provide many export potentials and have the potential to be developed into new cultivars [6].…”