In vitro maturation and in vitro fertilization of sheep oocytes

Wani, Nisar Ahmad

doi:10.1016/s0921-4488(02)00020-2

Cited by 40 publications

(60 citation statements)

References 48 publications

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“…When this method is combined with in vitro production (IVP), LOPU can produce a large number of viable offspring in a short time Wani, 2002;Cognié et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Cordeiro

Teixeira

Oliveira

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration [ ABSTRACTThe objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no complications were found during the laparoscopic procedures that would impair the reproductive future of the animals. Therefore, a viable number of oocytes were obtained even with the decreased reproductive efficiency, proving that repeated LOPUs do not interfere with the reproductive of adult and prepubertal nanny goats. These results indicate a positive aspect of this procedure, allowing for increasing reproductive performance of this kind, when used for the production in vitro.

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“…When this method is combined with in vitro production (IVP), LOPU can produce a large number of viable offspring in a short time Wani, 2002;Cognié et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Cordeiro

Teixeira

Oliveira

et al. 2014

Arq. Bras. Med. Vet. Zootec.

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“…Obviously, nuclear maturation of oocytes along with cytoplasmic maturation is important at the completion of meiotic division for success of fertilization [6]. Besides, being of nutritive value, serum nurtures the cells surrounding the oocyte rather than the oocyte itself and prevents the oocyte of the zone hardening when the oocyte is liberated from the follicular environments [20]. Several studies have demonstrated that culture medium supplemented with serum can cause morphological [27,28] and physiological [29,30] differences in embryos compared with those produced in vivo or in serum free media.…”

Section: Discussionmentioning

confidence: 99%

“…The COCs were isolated under a stero zoom microscope (Nikon Corporation. Japan) and graded as good, fair or poor [20]. Only good or fair oocytes were considered acceptable and used in IVM experiments.…”

Section: In Vitro Maturation (Ivm)mentioning

confidence: 99%

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

Shabankareh

Sarsaifi

Mehrannia

2010

J Assist Reprod Genet

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Objective To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes Method (s) Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development. Results Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.Conclusion Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.

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“…Prior to in vitro fertilization, COCs are collected either by minimally invasive laparoscopic ovum pick up (LOPC) or postmortem from slaughtered females [32,34]. Cumulus-oocyte complexes are collected from live sheep by a laparotomy followed by aspiration of oocytes from ovaries using an aspiration pump.…”

Section: Oocyte Collection and In Vitro Maturationmentioning

confidence: 99%