Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration

Abstract

Reproductive efficiency of adult and prepubertal goats subjected to repeated follicular aspiration [ ABSTRACTThe objective of this study was to assess the reproductive response of adult and prepubertal goats subjected to repeated laparoscopic ovum pick-up (LOPU). The study animals were divided into two groups, specifically, adult nanny goats (GA, n=10) and prepubertal nanny goats (GP, n=10), which were subjected to estrous synchronization and ovarian stimulation for LOPU. Both groups underwent six LOPU procedures at seven-day intervals and were subsequently subjected to controlled mating and pregnancy diagnosis to evaluate their future fertility. The study showed a reduction in the number of follicles visualized and in the amount and quality of the oocytes that were recovered and exposed to in vitro maturation. As indicated by the fertility test, however, no complications were found during the laparoscopic procedures that would impair the reproductive future of the animals. Therefore, a viable number of oocytes were obtained even with the decreased reproductive efficiency, proving that repeated LOPUs do not interfere with the reproductive of adult and prepubertal nanny goats. These results indicate a positive aspect of this procedure, allowing for increasing reproductive performance of this kind, when used for the production in vitro.

Laparoscopic ovum pick-up in spotted paca ( Cuniculus pacas )

“…Several authors reported oocyte recovery rates ranging from 16 to 98% (Alberio et al, 2002;Gibbons et al, 2007;Teixeira et al, 2011;Cordeiro et al, 2014) in small ruminants. Due to the shortage of LapOPU data in rodents, it can be assumed that the recovery rate achieved in our study (32.56±27.32%) is in accordance with what Rodríguez et al (2006) considered normal (from 32 to 90%).…”

“…Laparoscopic ovum pick-up (LapOPU) used for in vitro production (IVP) is one of the leading reproductive biotechniques in assisted reproduction, highlighted for being less invasive, provide a quicker recovery and the possibility of being held several times in the same female as already described in some domestic species (Teixeira et al, 2011;Cordeiro et al, 2014;Padilha et al, 2014;Teixeira et al, 2015).…”

Intraperitoneal lidocaine hydrochloride for prevention of intraperitoneal adhesions following laparoscopic genitourinary tract surgery in ewes

“…Another study reported the occurrence of intraperitoneal adhesions on the reproductive tract of 15% of goats following ovum pick-up, even though the organs were rinsed with heparin solution following surgery (Cordeiro et al 2014). Unilateral and bilateral ovarian adhesions were reported in ewes following 10 sessions of laparoscopic OPU, which were performed at seven-day intervals (Stangl et al 1999).…”

“…Although adhesions formed over the ovaries, those were considered easy to dissect and irrelevant for repeated OPU. The use of other anti-adhesion therapies, such as a heparin-based one (Baldassarre and Karatzas 2004;Cordeiro et al 2014) or carboxymethylcellulose solutions (Ewoldt et al 2004) have been recommended; however, even these solutions were not fully efficient in preventing adhesions.…”

“…Endosurgery is a minimally invasive procedure that greatly improves the success of reproduction biotechnologies, such as artificial insemination, embryo transfer and ovum pick-up in small ruminants (Teixeira et al 2013;Cordeiro et al 2014).…”