In vitro maturation of caprine oocytes in different concentrations of estrous goat serum

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

“…Sakaguchi et al [26] reported that cytoplasmic maturation of oocytes during maturation without serum supplementation might be incomplete, and some other factors (possibly some serum components) are necessary for completion of cytoplasmic maturation of ovine oocytes in the in vitro condition. Obviously, nuclear maturation of oocytes along with cytoplasmic maturation is important at the completion of meiotic division for success of fertilization [6]. Besides, being of nutritive value, serum nurtures the cells surrounding the oocyte rather than the oocyte itself and prevents the oocyte of the zone hardening when the oocyte is liberated from the follicular environments [20].…”

“…Consistently, successful and reliable oocyte maturation (both cytoplasmic and nuclear maturation) would dramatically improve the efficiency of preimplantation embryonic development as well as fetal development [2]. Numerous studies have examined the ability of mammalian oocytes and embryos to develop in vitro using a wide variety of culture media [3][4][5][6]. In most of the studies, the basic medium was supplemented with hormones and different concentration of sera.…”

“…The maturation medium and Capsule Media Supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. the selection of protein supplements and gonadotrophins for IVM play an important role in subsequent IVF and in vitro development [6].…”

Parthenogenesis and activation of mammalian oocytes for <i>in vitro</i> embryo production: A review

“…In-vitro maturation can be achieved by the supplementation of 20% estrous goat serum. Supplementation of 20% EGS or 10% FBS + 3 mg/ml BSA in TCM-199 medium could also be used to achieve maturation followed by fertilization, embryo culture, and subsequent embryo transfer resulted in successful birth of caprine kid [10,16,22]. In our previous study we found slightly better in-vitro maturation rate in the presence of antioxidant ß-mercaptoethanol as compared to base medium (TCM-199 with NCS and 3 mg/ml BSA) but lesser than base medium containing hormones (LH-5μg/ml, FSH-5 μg/ml, Estradiol 1 μg/ml) [21].…”

Efecto del malatión en la maduración in vitro de ovocitos porcinos y su asociación con el estrés oxidante

“…Antes de la maduración, el núcleo del ovocito se encuentra en estado de vesícula germinal (VG); en la etapa de rompimiento de la vesícula germinal (RVG) el nucléolo y membrana nuclear desaparecen y los cromosomas se condensan En la metafase I (MI) los pares de cromosomas son visibles y al final de la primera división meiótica, se libera el primer cuerpo polar y el ovocito queda detenido en la metafase II (MII) (Kharche et al, 2006). En la mayoría de los mamíferos la ovulación se lleva a cabo durante la metafase de la segunda división meiótica, sólo cuando el ovocito es fertilizado concluye la segunda dvisión meiótica y se expulsa el segundo cuerpo polar.…”