In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Uzun, Sati; Parmaksız, İskender; Uranbey, Serkan; Mirici, Semra; Sarihan, Ercument Osman; İpek, Ahmet; Kaya, Muammer; Gürbüz, Bilal; Arslan, Neşet; Sancak, Cengiz; Khawar, Khalid Mahmood; Özcan, Sebahattin

doi:10.3906/biy-1307-52

Cited by 13 publications

(19 citation statements)

References 20 publications

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“…The results of the study suggest that the indvidual regeneration on TDZ-NAA-containing MS medium was strongly influenced by the concentrations and combinations of plant growth regulators. The results are in agreement with Uranbey (2010 a, b), Uranbey et al (2011), Ozel et al (2007, 2009, 2015, Vaziri et al (2014) and Uzun et al (2014), who reported the propagation of other Turkish Muscari species using different cytokinins and auxin concentrations.…”

Section: Bulblet Regeneration Behavioursupporting

confidence: 90%

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Özel

Ünal

2016

Turkish JAF Sci.Tech.

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Muscari neglectum Guss. Ex Ten, is an ornamental, herbaceous perennial plant species that grows in the Mediterranean countries with attractive and scented blue-colored flowers. The plant has low seed output, seed dormancy, low germination and propagation rates. This study aimed to develop a reliable microclonal propagation protocol for M. neglectum using TDZ (Thidiazuron)-NAA (α Naphthalene acetic acid) to induce bulblets, roots, and acclimatization of the regenerated bulblets. Maximum number of bulblets per explant (8.25±0.05) was noted on MS medium containing 0.0454 µM TDZ-5.37 µM NAA. The bulblets regenerated in each type of culture medium were very vigorous, and acclimatized easily following rooting on a subculture. Here we show that this protocol is a useful clonal micropropagation system for this important ornamental plant.

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Section: Bulblet Regeneration Behavioursupporting

confidence: 90%

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Özel

Ünal

2016

Turkish JAF Sci.Tech.

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“…Contrarily, Petric et al (2011) revealed the importance of a low TDZ concentration of 0.05-0.50 mg/L for optimum bulblet formation for F. melegris. However, negative effects of a higher TDZ concentration (2.0 mg/L) on bulblet formation and bulblet numbers of M. muscarimi Medic (Uzun et al, 2014b) have also been reported. In vitro regenerated bulblets from germination or regeneration experiments failed to acclimatize in pots containing peat moss, perlite, or vermiculite alone or in different combinations (data not shown).…”

Section: Discussionmentioning

confidence: 99%

Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica

Çakmak¹,

Karaoğlu²,

Aasım³

et al. 2016

Turk J Biol

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IntroductionThe genus Fritillaria (Liliaceae) is an important geophytic taxon with more than 100 species and wide distribution in different climatic zones ranging from southern Europe to the Middle East (Le Nard and De Hertogh, 1993), including Turkey (Bryan, 2002). In Turkey, there are 35 species representing 48 taxa of Fritillaria, and it is ranked 4th after Allium, Iris, and Crocus. Most of the Fritillaria species in Turkey are endemic. The plants flower during spring and are widely accepted as ornamental plants due to their attractive flowers (Le Nard and De Hertogh, 1993). Besides that, the Fritillaria species are used as medicinal plants (Rønsted et al., 2005;Wang et al., 2005) in traditional Chinese medicine (Li et al., 2001) due to a wide array of alkaloids with interesting phytochemical properties.Persian lily (Fritillaria persica) is found mainly in Hatay, Mersin, and Adıyaman provinces of Turkey, as well as in Lebanon, Jordan, and Iran at 700 m to 2800 m above sea level. The bulbs are 3-5 cm in diameter with 2-3 succulent leaves. The plant has 10-25 leaves, which are approximately 15 cm long and 3 cm wide. It bears 7-20 purple bell-shaped flowers, growing in racemose position. The plant can grow up to 20-60 cm in height (Ulug et al., 2010).Conventional plant production methods currently used for the Fritillaria species are seed or bulb propagation. These propagation techniques have certain disadvantages such as very low or unpredictable germination of seeds due to physiological dormancy (Baskin and Baskin, 2004), with weak seedlings, low survival rate, and slow growth, which may take 3-4 years for bulblet maturation (Le Nard and De Hertogh, 1993). Apart from this, a limited availability of bulblets from nature (Subotić et al., 2010) also makes it difficult to get sufficient material to propagate these ornamental species. These limitations suggest the need to develop alternative propagation methods (Ulug et al., 2010) for commercial production of these valuable species.A number of regeneration protocols have been reported for different Fritillaria species by

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“…Similarly, there are some reports concerning somatic embryogenesis in bulb explants of M. neglectum inoculated with various concentrations of BA alone or in combination with NAA (Karamian et al, 2010) and encapsulation of somatic embryos obtained from protoplast cultures (Karamian et al, 2011). Recently, Uzun et al (2014) reported the best bulblet regeneration in M. muscarimi using MS medium containing 4.0 mg/L BA and 0.50 mg/L NAA after 1 year of culture initiation. On the other hand, there have been several reports on tissue culture studies via leaf and callus cultures derived from flower buds (Suzuki and Nakano, 2001;Mori and Nakano, 2004;Azad and Amin, 2012), protoplast cultures of the Muscari cultivar Blue Pearls (Nakano et al, 2005), and Agrobacterium-mediated production of transgenic M. armeniacum (Suzuki and Nakano, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…Tissue culture applications might be appropriate for the bulblet production in vitro and might also be assessed as a prevailing instrument for ex situ conservation of geophytes against their intensive collection from natural habitats. However, micropropagation of several Muscari species, such as M. racemosum (Kromer, 1989), M. macrocarpum Sweet (Ozel et al, 2007), M. aucheri (Uranbey, 2010a), M. azureum (Uranbey, 2010b), M. mirium (Nasırcılar et al, 2011), and M. muscarimi (Uzun et al 2014), has been established. There are also some reports concerning plantlet regeneration of M. armeniacum via callus cultures (Suzuki and Nakano, 2001;Mori and Nakano, 2004;Azad and Amin, 2012) and protoplast cultures (Nakano et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…M. armeniacum, belonging to the family Asparagaceae, is cultivated in pots and gardens in the temperate regions. Traditional methods of propagation of Muscari species are rather slow, since the bulblet production from the mother bulbs is extremely low (Uzun et al, 2014). Tissue culture applications might be appropriate for the bulblet production in vitro and might also be assessed as a prevailing instrument for ex situ conservation of geophytes against their intensive collection from natural habitats.…”

Section: Introductionmentioning

confidence: 99%

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Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

Yücesan¹,

Çiçek²,

Gürel³

2014

Turk J Agric For

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An efficient in vitro regeneration system was developed for the ornamental Muscari armeniacum on Linsmaier and Skoog basal medium (LS) supplemented with benzyladenine (BA) at varying concentrations (0.5, 1.0, or 2.0 mg/L) alone or in combination with 0.5 mg/L α-naphthalene acetic acid (NAA). The highest mean number of direct somatic embryo formations was observed on LS medium containing 2.0 mg/L BA and 0.5 mg/L NAA, with a mean of 7.9 somatic embryos per explant after 10 weeks of culture. Green nodular calli induced on LS medium containing 5.0 mg/L BA alone or in combination with 0.5 mg/L NAA were transferred to LS medium supplemented with or without 0.5 mg/L gibberellic acid (GA3) for 8 weeks, producing 23.3 immature bulblets. Immature bulblets produced in vitro were either embedded in a sodium alginate matrix for the encapsulation process or were transferred directly to LS medium supplemented with or without GA 3 at 0.5 or 1.0 mg/L for growth and development for 6 weeks. Encapsulated bulblets were then stored at 4 °C in darkness for 10 weeks and almost all encapsulated bulblets retained their viability and resumed their growth under nonaxenic greenhouse conditions.

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In vitro micropropagation from immature embryos of the endemic and endangered Muscari muscarimi Medik.

Cited by 13 publications

References 20 publications

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid

Advancement in protocol for in vitro seed germination, regeneration, bulblet maturation, and acclimatization of Fritillaria persica

Somatic embryogenesis and encapsulation of immature bulblets of an ornamental species, grape hyacinths (Muscari armeniacum Leichtlin ex Baker)

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