In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

Huq, Md. Amdadul; Akter, Shahina; Islam, Saidul; Khan, Salim

doi:10.3329/bjsir.v47i2.11457

Cited by 5 publications

(2 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar results were found by Canli and Tian [31] in Prunus salicina Lind1. In contrast, Thomas and Sreejesh [32], and Huq et al [33] working respectively on ash gourd (Benincasa hispida L.) and pointed gourd (Trichosanthes dioica Roxb.) noticed the variation in shoot induction and plant micropropagation according to BAP concentrations.…”

Section: Effects Of 6-benzylaminopurine (Bap) Concentrationmentioning

confidence: 98%

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

Gnamien¹,

Bi²,

Kouadio³

et al. 2013

View full text Add to dashboard Cite

To regenerate adventitious shoots from the cotyledon proximal parts of Citrullus lanatus (Thunb.) Matsum. and Nakai ssp. mucosospermus (Fursa) oleaginous type, different concentrations of MS mineral elements, sucrose, 6-benzylaminopurine (BAP) and agar were tested. Shoot induction proved to depend on the interaction between levels of sucrose, BAP and MS mineral elements in the medium. The medium containing 3/2 strength of MS mineral elements, 35 g/l sucrose and 1 mg/l BAP solidified with 6 g/l agar allowed the production of numerous shoots without a callus phase. After 3 weeks of culture, 76.7% of the cotyledon proximal parts induced shoots with an average of 12.26 shoots per explant and a mean shoot length of 17.13 mm. The induced shoots were directly rooted and thus complete plants ready for acclimatization were obtained using a two steps procedure. Depending on the genotype, the shoot induction from cotyledon proximal parts ranged from 54% to 96%. Rooted plantlets were acclimatized and transferred to field, where they grew well, developed flowers and fruits like seeded plants. The assessment of the genetic stability of the in-vitro-regenerated plantlets by means of an Amplified Fragment Length Polymorphism (AFLP) analysis with the combination of 5 primers revealed no differences between regenerated plantlets and mother plants.

show abstract

Section: Effects Of 6-benzylaminopurine (Bap) Concentrationmentioning

confidence: 98%

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

Gnamien¹,

Bi²,

Kouadio³

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Among these, stem segments are frequently employed to induce cluster buds. Huq et al (2012) observed that when treating the stem tips and stem segments of Trichosanthes dioica Roxb. under the same medium conditions, the regeneration, proliferation, and elongation of stem segments were superior to those of stem tips.…”

Section: Bud Induction In Vitro Culturementioning

confidence: 99%

In vitro cluster buds regeneration and control of Shoot Tip Necrosis in tissue cultures of Trichosanthes cucumerina Linn.

LIU,

DENG,

YAN

et al. 2024

Preprint

View full text Add to dashboard Cite

Trichosanthes cucumerina Linn. is a medicinal vine with great potential that can be used as a vegetable. In this study, an efficient in vitro regeneration system of T. cucumerina was established by inducing adventitious buds and controlling Shoot Tip Necrosis (STN) in vitro culture. The results of hormone single factor and orthogonal experiments showed that 6-BA had a significant effect on inducing adventitious buds. The best medium for bud proliferation was MS + 6-BA 3.0 mg·L-1 + KT 2.0 mg·L-1 + NAA 0.5 mg·L-1, and the proliferation coefficient was 14.68. However, after three consecutive subcultures, a higher incidence of STN (78.86%) was observed. The results of the STN control experiment showed that reducing the concentration of inorganic salt, shortening the subculture interval, and increasing the Ca2+ in the medium could effectively control the occurrence of STN. When the subculture interval was 40 d, the stem segments obtained the lowest STN incidence (8.3%) and higher shoot proliferation coefficient (10.02) on the medium of MS + 3.0 mg·L-1 6-BA + 2.0 mg·L-1 KT + 0.5 mg·L-1 NAA + 360.36 mg·L-1 Ca2+. The best rooting medium for stem segments was MS + 0.50 mg·L-1 NAA; the rooting rate was 100%, and no STN occurred. After acclimation, the survival rate of the rooted plantlet reached 95% after 40 days in the soil matrix of coconut husk: humus soil: perlite = 2: 2: 1 (v/v). The results of this study not only reveal the mechanism of STN in T. cucumerina, but also provide technical support and a theoretical basis for its further artificial breeding and cultivation.

show abstract

In vitro cluster buds regeneration and control of shoot tip necrosis in tissue cultures of Trichosanthes cucumerina L.

Liu,

Deng,

Yan

et al. 2024

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Trichosanthes cucumerina L. is a medicinal vine with great potential that can be used as a vegetable. In this study, an efficient in vitro regeneration system of T. cucumerina was established by inducing adventitious buds and controlling Shoot Tip Necrosis (STN) in the in vitro culture. The results of hormone single factor and orthogonal experiments showed that 6-BA had a significant effect on inducing adventitious buds. The best medium for bud proliferation was MS + 6-BA 3.0 mg•L − 1 + KIN 2.0 mg•L − 1 + NAA 0.5 mg•L − 1 , and the proliferation capacity was 14.68. However, after three consecutive subcultures, a higher incidence of STN (78.86%) was observed. The results of the STN control experiment showed that reducing the concentration of inorganic salt, shortening the subculture interval, and increasing the Ca 2+ in the medium could effectively control the occurrence of STN. However, reducing the concentration of inorganic salt and shortening the subculture interval excessively reduces the proliferation capacity significantly. Considering both the incidence of STN and proliferation capacity, the measures proposed in this study to alleviate STN were to keep the concentration of inorganic salt unchanged, adjust the subculture interval from 45 days to 40 days, and increase the Ca 2+ concentration in the medium to 480 mg•L − 1 . When the subculture interval was 40 d, the stem segments obtained the lowest STN incidence (8.3%) and higher shoot proliferation capacity (10.02) on the medium of MS + 3.0 mg•L − 1 6-BA + 2.0 mg•L − 1 KIN + 0.5 mg•L − 1 NAA + 360.36 mg•L − 1 Ca 2+ (360 mg•L − 1 is the concentration of additional calcium to be added, and after this addition, the total calcium concentration in the MS medium was 480.48 mg•L − 1 ). The best rooting medium for stem segments was MS + 0.50 mg•L − 1 NAA; the rooting rate was 100%, and no STN occurred. After acclimation, the survival rate of the rooted plantlet reached 95% after 40 days in the soil matrix of coconut husk: humus soil: perlite = 2: 2: 1 (v/v). The results of this study not only reveal the mechanism of STN in T. cucumerina, but also provide technical support and a theoretical basis for its further breeding and cultivation. Key messageIn this study, an efficient and stable tissue culture rapid propagation system for Trichosanthes cucumerina L. was formed by controlling the shoot tip necrosis in vitro culture.

show abstract

In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

Cited by 5 publications

References 8 publications

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

In vitro cluster buds regeneration and control of Shoot Tip Necrosis in tissue cultures of Trichosanthes cucumerina Linn.

In vitro cluster buds regeneration and control of shoot tip necrosis in tissue cultures of Trichosanthes cucumerina L.

Contact Info

Product

Resources

About

In vitro micropropagation of pointed gourd (Trichosanthes dioica Roxb.) from shoot tip and nodal segment

Cited by 5 publications

References 8 publications

Medium effects on micropropagation and genetic stability of &lt;i&gt;Citrullus lanatus&lt;/i&gt; oleaginous type

Medium effects on micropropagation and genetic stability of &lt;i&gt;Citrullus lanatus&lt;/i&gt; oleaginous type

In vitro cluster buds regeneration and control of Shoot Tip Necrosis in tissue cultures of Trichosanthes cucumerina Linn.

In vitro cluster buds regeneration and control of shoot tip necrosis in tissue cultures of Trichosanthes cucumerina L.

Contact Info

Product

Resources

About

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type

Medium effects on micropropagation and genetic stability of <i>Citrullus lanatus</i> oleaginous type