In vitro mineralization of a three-dimensional collagen matrix by human dental pulp cells in the presence of chondroitin sulphate

Bouvier, Martine; Joffre, A.; Magloire, H.

doi:10.1016/0003-9969(90)90047-e

Cited by 40 publications

(20 citation statements)

References 26 publications

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“…In the current study, collagen sponges experienced a very high degradation process, with the highest ratios of weight loss in both lysozyme and collagenase solutions (Figs. [2][3][4][5]. This finding indicates that collagen sponges have low biological stability 20,21,24,25 .…”

Section: Discussionmentioning

confidence: 78%

Properties of chitosan–collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells

Arpornmaeklong

Pripatnanont

Suwatwirote

2008

International Journal of Oral and Maxillofacial Surgery

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Section: Discussionmentioning

confidence: 78%

Properties of chitosan–collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells

Arpornmaeklong

Pripatnanont

Suwatwirote

2008

International Journal of Oral and Maxillofacial Surgery

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“…Corresponding to mineralization of nodules, the calcium content in the cell layers began to significantly increase from day 15. Although several experiments have demonstrated that dental pulp cells formed mineralized nodules in long-term cultures [3,4,24,25], actually only limited investigation has been made concerning the dentin formation. Chung et al [26] reported that a cell-free in vitro system formed mineral particles in the presence of high amounts of ALP and ␤-glycerophosphate and that this mineralization was merely the deposition of calcium.…”

Section: Discussionmentioning

confidence: 98%

Establishment and Characterization of a Culture System for Enzymatically Released Rat Dental Pulp Cells

Yokose

Kadokura

Tajima

et al. 2000

Calcified Tissue International

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To establish a cell culture system that reflects the dentin formation in dental pulp tissue, we used dental pulp cells enzymatically isolated from rat incisor teeth. During the 20-day culture period, the cells exhibited various phenotypes of the odontoblast differentiation process, from the immature stage to the terminal mineralization stage. The cells began to form the mineralized nodules from day 10, and the nodules became larger by day 20. Alkaline phosphatase (ALP)-positive cells surrounded the mineralized nodules. The ALP activity in the cell layers was maximal on day 5, and gradually decreased to day 20. The calcium content in the cell layers was very low by day 10, and significantly increased from day 15. Sulfated glycosamino-glycans (GAGs) contained in the cell layers increased by day 15, but the content then decreased by day 20. The dental pulp cells produced a small amount of osteocalcin that was released into the culture medium by day 10, and the amount secreted increased markedly from day 15. The expression of osteocalcin and parathyroid hormone/parathyroid hormone-related peptide (PTH/PTHrP) receptor mRNA was evident as early as day 5, and the expression of each gradually increased with the number of days in culture. Dentin matrix protein (Dmp1) mRNA gene transcripts were identified by use of the reverse transcription polymerase chain reaction (RT-PCR) in the cells throughout the culture period. The present results demonstrate that this culture system is useful for studying the differentiation process of the odontoblast-like cells.

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“…Investigators have carried out experimental attempts in vitro to induce differentiation of pulp cells by culturing mouse dental papillae in agar microinjected with active molecules (Begue-Kirn, 1992, or more easily by obtaining long-term cultured cells from adult pulps (Tziafas et al, 1988;Bouvier et al, 1990;Veron et al, 1990;Nakashima, 1991Nakashima, , 1992Seux et al, 1991;Liang et al, 1992;Alliot-Licht et al, 1994;Nakashima et al, 1994;Shirakawa et al, 1994) grown on various substrates (collagen, fibronectin, glycosaminoglycans, calcium hydroxide etc.) or in the presence of growth factors (BMPs, TGFfs, EGF).…”

Section: Introductionmentioning

confidence: 99%

An in vitro Model of Human Dental Pulp Repair

Magloire¹,

Joffre²,

Bleicher³

1996

J Dent Res

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Abstract. Pulp tissue responds to dentin injury by laying down reactionary dentin secreted by existing odontoblasts or reparative dentin elaborated by odontoblast-like cells that differentiated from precursor cells in the absence of inner dental epithelium and basement membrane. Furthermore, growth factors or active dentin matrix components are fundamental signals involved in odontoblast differentiation. In vitro, dental pulp cells cultured under various conditions are able to express typical markers of differentiation, but no culture system can re-create pulp response to dentin drilling. This paper reports the behavior of thick slices from human teeth drilled immediately after extraction and cultured from 3 days to 1 month. Results show that the damaged pulp beneath the cavity is able to develop, in vitro, some typical aspects correlated to tissue healing, evidenced by cell proliferation (BrdU-positive cells), neovascularization (positive with antitype-IV collagen antibodies), and the presence of functional (3H proline-positive) cuboidal cells close to the injured area.After 30 days of culture, elongated spindle-shaped cells can be seen aligned along the edges of the relevant dentin walls, whereas sound functional odontoblasts are well-preserved beneath healthy areas. This tissue recovery leads us to believe that such a culture model will be a useful system for testing factors regulating pulp repair.

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In vitro mineralization of a three-dimensional collagen matrix by human dental pulp cells in the presence of chondroitin sulphate

Cited by 40 publications

References 26 publications

Properties of chitosan–collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells

Properties of chitosan–collagen sponges and osteogenic differentiation of rat-bone-marrow stromal cells

Establishment and Characterization of a Culture System for Enzymatically Released Rat Dental Pulp Cells

An in vitro Model of Human Dental Pulp Repair

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