In Vitro Models of Intestine Innate Immunity

Roh, Terrence T.; Chen, Ying; Rudolph, Sara; Gee, Michelle; Kaplan, David L.

doi:10.1016/j.tibtech.2020.07.009

Cited by 24 publications

(13 citation statements)

References 79 publications

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“…Additionally, while organoids provide heterogeneity closer to that seen in vivo, this heterogeneity can make it difficult to compare results among experiments and labs. Factors such as Matrigel batch, media composition, and patient variability affect organoid growth and development and lead to confounding results. , Immortalized cell-line models can be grown rapidly, inexpensively, and reduce variability such that other factors like proliferation, differentiation, and polarization, can be examined for optimization of culture conditions. , As a preliminary study, we aimed to have low variability and low cost and therefore opted to use a combination of Caco2 and HT29-MTX to represent the epithelial layer of cells.…”

Section: Discussionmentioning

confidence: 99%

“…56,57 Immortalized cell-line models can be grown rapidly, inexpensively, and reduce variability such that other factors like proliferation, differentiation, and polarization, can be examined for optimization of culture conditions. 58,59 As a preliminary study, we aimed to have low variability and low cost and therefore opted to use a combination of Caco2 and HT29-MTX to represent the epithelial layer of cells.…”

Section: Cell Source Of Intestinal Epithelial Cells For Intestine Tis...mentioning

confidence: 99%

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Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium

Rudolph

Longo

Tse

et al. 2022

ACS Biomater. Sci. Eng.

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Crypt-villus architecture in the small intestine is crucial for the structural integrity of the intestinal epithelium and maintenance of gut homeostasis. We utilized three-dimensional (3D) printing and inverse molding techniques to form threedimensional (3D) spongy scaffold systems that resemble the intestinal crypt-villus microarchitecture. The scaffolds consist of silk fibroin protein with curved lumens with rows of protruding villi with invaginating crypts to generate the architecture. Intestinal cell (Caco-2, HT29-MTX) attachment and growth, as well as long-term culture support were demonstrated with cell polarization and tissue barrier properties compared to two-dimensional (2D) Transwell culture controls. Further, physiologically relevant oxygen gradients were generated in the 3D system. The various advantages of this system may be ascribed to the more physiologically relevant 3D environment, offering a system for the exploration of disease pathogenesis, host−microbiome interactions, and therapeutic discovery.

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Section: Discussionmentioning

confidence: 99%

Section: Cell Source Of Intestinal Epithelial Cells For Intestine Tis...mentioning

confidence: 99%

Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium

Rudolph

Longo

Tse

et al. 2022

ACS Biomater. Sci. Eng.

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“…A significant role in maintenance of a healthy gut barrier is played by the GM. Cell models of host-microbe interactions and innate immune functions is a step closer towards physiological relevance (Roh et al, 2021). Combining iPSC technology with GM-models will likely add significant knowledge on top of existing research from primary cells co-cultured with GM (Zhang et al, 2021).…”

Section: Gut Microbiota and Intestinal Cell Modelsmentioning

confidence: 99%

“…Increasingly advanced scientific and technical developments make us able to mimic complex physiological functions by combining different cell types and formats. Such models include organoids (Dutta et al, 2017;Lehmann et al, 2019;Kim et al, 2020), multi-well systems (Baudoin et al, 2012;Dutta et al, 2017;Roh et al, 2021), as well as microphysiological organ-on-a-chip (OoC) models (Vernetti et al, 2017;Ronaldson-Bouchard and Vunjak-Novakovic, 2018;Zhang et al, 2018). Organoids are multicellular 3D spheres that differentiate and self-organize to reconstruct the features of cell-cell interactions of specific tissues (Dutta et al, 2017;Liu et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Getting closer to modeling the gut-brain axis using induced pluripotent stem cells

Hall

Bendtsen

2023

Front. Cell Dev. Biol.

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The gut microbiome (GM), the gut barrier, and the blood-brain barrier (BBB) are key elements of the gut-brain axis (GBA). The advances in organ-on-a-chip and induced pluripotent stem cell (iPSCs) technology might enable more physiological gut-brain-axis-on-a-chip models. The ability to mimic complex physiological functions of the GBA is needed in basic mechanistic research as well as disease research of psychiatric, neurodevelopmental, functional, and neurodegenerative diseases, such as Alzheimer’s disease and Parkinson’s disease. These brain disorders have been associated with GM dysbiosis, which may affect the brain via the GBA. Although animal models have paved the way for the breakthroughs and progression in the understanding of the GBA, the fundamental questions of exactly when, how, and why still remain unanswered. The research of the complex GBA have relied on equally complex animal models, but today’s ethical knowledge and responsibilities demand interdisciplinary development of non-animal models to study such systems. In this review we briefly describe the gut barrier and BBB, provide an overview of current cell models, and discuss the use of iPSCs in these GBA elements. We highlight the perspectives of producing GBA chips using iPSCs and the challenges that remain in the field.

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“…The intestine plays an important role in barrier maintenance, digestion and absorption of nutrients, and immunity. 12,13 Heat stress induces changes in intestinal morphology and destroys the integrity of the intestinal barrier. 8,14 The possible mechanisms for this are the destruction of tight junctions (TJs) between epithelial cells and the impairment of goblet cell function.…”

Section: Introductionmentioning

confidence: 99%

Mogroside-rich extract from Siraitia grosvenorii fruits protects against heat stress-induced intestinal damage by ameliorating oxidative stress and inflammation in mice

Liu

et al. 2023

Food Funct.

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In Vitro Models of Intestine Innate Immunity

Cited by 24 publications

References 79 publications

Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium

Crypt-Villus Scaffold Architecture for Bioengineering Functional Human Intestinal Epithelium

Getting closer to modeling the gut-brain axis using induced pluripotent stem cells

Mogroside-rich extract from Siraitia grosvenorii fruits protects against heat stress-induced intestinal damage by ameliorating oxidative stress and inflammation in mice

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