In vitro monitoring of nanogram levels of naproxen in human urine using flow injection chemiluminescence

Abstract

Based on the synergic effect of the hydrophobicity of sodium dodecylbenzenesulfonate (SDBS) micelles and the incorporation of naproxen molecules, a simple, rapid and sensitive chemiluminescence (CL) method was described for the determination of naproxen in pharmaceuticals and urine. Under the optimum experimental conditions, the CL intensity was linear over the concentration of naproxen ranging from 1.0 to 700.0 ng mL −1 (r = 0.9917) with a detection limit as low as 0.9 ng mL −1 (S/N = 3). The mechanism of CL was discussed in detail based on the spectrometry. The experimental results demonstrated that the excretive amounts of naproxen reached its maximum in 4.0 h after taking 200 mg naproxen tablet; and the naproxen excretive ratio during 8.0 h was 5.46% in the body of volunteers.

Silver Nanoparticle-Enhanced Chemiluminescence Method for Determining Naproxen Based on Europium(III)-Sensitized Ce(IV)-Na2S2O4 Reaction

“…The above explanation further supports the CL reaction mechanism. Chemiluminescence (Ce(IV) in acidic medium) 100-1,000 ng mL −1 15 ng mL −1 [17] Chemiluminescence (sulfite-SDBS system in HCl)…”

“…Several analytical methods have been reported for determining NAP including spectrophotometry [2,3], fluorometry [4,5], synchronous spectrometry [6], phosphorimetry [7], voltammetry [8,9], liquid chromatography [10,11], capillary electrophoresis [12], high performance liquid chromatography (HPLC) [13][14][15], and chemiluminescence (CL) [16][17][18][19][20]. Among the above methods, CL coupled with flow injection offers the advantages of equipment simplicity, high detection sensitivity, low background noise and good reproducibility.…”

Challenges in the simultaneous quantitation of sumatriptan and naproxen in human plasma: Application to a bioequivalence study

“…A critical comparison of different analytical methods for identification and determination of several triptans including SUM has been reviewed by Saka [23]. Like SUM, there are numerous methods for the determination of NAP in biological fluids by a variety of analytical techniques like capillary electrophoresis [24], flow injection chemiluminescence [25], room temperature liquid phosphorimetry [26], electrokinetic capillary chromatography [27], fluorescence spectrometry [28][29][30][31][32], GC-MS [33], HPLC with chemiluminescence [34], fluorescence [33,[35][36][37][38] and UV detection [33,36,[39][40][41][42][43][44][45][46] and LC-MS/MS [46][47][48]. NAP has been determined in human plasma and dried blood spots by LC-MS/MS for pharmacokinetic comparison in the concentration range of 0.5-100 g/mL [47].…”

Chemiluminescence determination of naproxen based on europium(III)‐sensitized KIO4–H2O2 reaction

“…Various analytical methods have been reported for the determination of naproxen in pharmaceutical preparations and in biological fluids, including spectrophotometry, [3] fluorometry, [4,5] phosphorimetry, [6] voltammetry, [7,8] liquid chromatography, [9] capillary electrophoresis [10] and CL methods, [11][12][13][14] which were based on the CL reactions of KMnO 4 -Na 2 SO 3 , [11] Na 2 SO 3 -SDBS, [12] Ru(bpy) 3 2+ -PbO 2 [13] or direct oxidation with Ce(IV). [14] The trivalent lanthanide ions, especially europium(III) ion and terbium(III) ion, have recently been shown to be the useful CL sensitizers for chemical and biological applications.…”